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Article

Downregulation of aryl hydrocarbon receptor expression decreases gastric cancer cell growth and invasion

  • Authors:
    • Xiao-Fei Yin
    • Jie Chen
    • Wei Mao
    • Yu-Hong Wang
    • Min-Hu Chen
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China
  • Pages: 364-370
    |
    Published online on: April 22, 2013
       https://doi.org/10.3892/or.2013.2410
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Abstract

Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor associated with tumor initiation and progression. AhR expression is significantly increased in gastric cancer tissues and gastric cancer cell lines; however, the relationship between AhR and gastric cancer is still unclear. In the present study, we explored the effects of the inhibition of AhR expression by RNA interference on the biological behavior of gastric cancer cells (MKN45 and SGC7901), and elucidated the specific mechanisms of AhR action in the development of gastric cancer. Results showed that small interfering RNA (siRNA) against AhR effectively inhibited the expression of AhR, and decreased the expression of cytochrome P450 (CYP)1A1 and CYP1B1, which are classic target genes of the AhR pathway. Compared to the negative control group, AhR-siRNA-transfected cells showed decreased cellular growth, delayed G1-S cell cycle progression and increased apoptosis rate. Furthermore, inhibition of AhR expression by siRNA in SGC7901 cells led to decreased cell migratory and invasive ability, accompanied by downregulation of expression and activity of matrix metalloproteinase (MMP)-2 and MMP-9. Our results, therefore, suggest that AhR promotes the growth and invasiveness of gastric cancer cells and AhR may serve as a promising therapeutic target for gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Yin X, Chen J, Mao W, Wang Y and Chen M: Downregulation of aryl hydrocarbon receptor expression decreases gastric cancer cell growth and invasion. Oncol Rep 30: 364-370, 2013.
APA
Yin, X., Chen, J., Mao, W., Wang, Y., & Chen, M. (2013). Downregulation of aryl hydrocarbon receptor expression decreases gastric cancer cell growth and invasion. Oncology Reports, 30, 364-370. https://doi.org/10.3892/or.2013.2410
MLA
Yin, X., Chen, J., Mao, W., Wang, Y., Chen, M."Downregulation of aryl hydrocarbon receptor expression decreases gastric cancer cell growth and invasion". Oncology Reports 30.1 (2013): 364-370.
Chicago
Yin, X., Chen, J., Mao, W., Wang, Y., Chen, M."Downregulation of aryl hydrocarbon receptor expression decreases gastric cancer cell growth and invasion". Oncology Reports 30, no. 1 (2013): 364-370. https://doi.org/10.3892/or.2013.2410
Copy and paste a formatted citation
x
Spandidos Publications style
Yin X, Chen J, Mao W, Wang Y and Chen M: Downregulation of aryl hydrocarbon receptor expression decreases gastric cancer cell growth and invasion. Oncol Rep 30: 364-370, 2013.
APA
Yin, X., Chen, J., Mao, W., Wang, Y., & Chen, M. (2013). Downregulation of aryl hydrocarbon receptor expression decreases gastric cancer cell growth and invasion. Oncology Reports, 30, 364-370. https://doi.org/10.3892/or.2013.2410
MLA
Yin, X., Chen, J., Mao, W., Wang, Y., Chen, M."Downregulation of aryl hydrocarbon receptor expression decreases gastric cancer cell growth and invasion". Oncology Reports 30.1 (2013): 364-370.
Chicago
Yin, X., Chen, J., Mao, W., Wang, Y., Chen, M."Downregulation of aryl hydrocarbon receptor expression decreases gastric cancer cell growth and invasion". Oncology Reports 30, no. 1 (2013): 364-370. https://doi.org/10.3892/or.2013.2410
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