Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
July 2013 Volume 30 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July 2013 Volume 30 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Protein-bound polysaccharide-K induces apoptosis via mitochondria and p38 mitogen‑activated protein kinase‑dependent pathways in HL‑60 promyelomonocytic leukemia cells

  • Authors:
    • Noriyuki Hirahara
    • Takeo Edamatsu
    • Ayako Fujieda
    • Masaki Fujioka
    • Tsutomu Wada
    • Yoshitsugu Tajima
  • View Affiliations / Copyright

    Affiliations: Department of Digestive and General Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693‑8501, Japan, Biomedical Research Laboratories, Kureha Corporation, Tokyo 169-8503, Japan
  • Pages: 99-104
    |
    Published online on: April 22, 2013
       https://doi.org/10.3892/or.2013.2412
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Protein-bound polysaccharide-K (PSK) is extracted from Coriolus versicolor (CM101). PSK is a biological response modifier (BRM), and its mechanism of action is partly mediated by modulating host immune systems; however, recent studies showed antiproliferative activity of PSK. Therefore, we examined the mechanism underlying the antiproliferative activity of PSK using seven different human malignant cell lines (WiDr, HT29, SW480, KATOⅢ, AGS, HL-60 and U937), and PSK was found to inhibit the proliferation of HL-60 cells most profoundly. Therefore, HL-60 cells were used to elucidate the mechanism of the antiproliferative activity. Western blotting was performed to detect phosphorylated p38 mitogen-activated protein kinase (MAPK). A p38 MAPK inhibitor, SB203580, was used to examine the roles in PSK-induced apoptosis and growth inhibition. Flow cytometry was performed for mitochondrial membrane potential detection. PSK activated caspase-3 and induced p38 MAPK phosphorylation. Co-treatment with SB203580 blocked PSK-induced apoptosis, caspase-3 activation and growth inhibition. PSK induced apoptosis via the mitochondrial pathway. The depolarization of mitochondria induced by PSK was reversed by co-treatment with SB203580. The present study revealed that PSK induced apoptosis in HL-60 cells via a mitochondrial and p38 MAPK-dependent pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Tsukagoshi S, Hashimoto Y, Fujii G, Kobayashi H, Nomoto K and Orita K: Krestin (PSK). Cancer Treat Rev. 11:131–155. 1984. View Article : Google Scholar

2 

Nakazato H, Koike A, Saji S, Ogawa N and Sakamoto J: Efficacy of immunochemotherapy as adjuvant treatment after curative resection of gastric cancer. Lancet. 343:1122–1126. 1994. View Article : Google Scholar : PubMed/NCBI

3 

Ohwada S, Ikeya T, Yokomori T, et al: Adjuvant immunochemotherapy with oral tegafur/uracil plus PSK in patients with stage II or III colorectal cancer: a randomized controlled study. Br J Cancer. 90:1003–1010. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Konno K, Motomiya M, Oizumi K, et al: Effects of protein-bound polysaccharide preparation (PSK) in small cell carcinoma of the lung. Haigan. 28:19–28. 1988. View Article : Google Scholar

5 

Iguchi C, Nio Y, Takeda H, et al: Plant polysaccharide PSK: cytostatic effects on growth and invasion; modulating effect on the expression of HLA and adhesion molecules on human gastric and colonic tumor cell surface. Anticancer Res. 21:1007–1013. 2001.

6 

Hirahara N, Fujioka M, Edamatsu T, et al: Protein-bound polysaccharide-K (PSK) induces apoptosis and inhibits proliferation of promyelocytic leukemia HL-60 cells. Anticancer Res. 31:2733–2738. 2011.PubMed/NCBI

7 

Evan GI and Vousden KH: Proliferation, cell cycle and apoptosis in cancer. Nature. 411:342–348. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Hu W and Kavanagh JJ: Anticancer therapy targeting the apoptosis pathway. Lancet Oncol. 4:721–729. 2003. View Article : Google Scholar

9 

Yefenof E, Gafanovitch I, Oron E, Bar M and Klein E: Prophylactic intervention in radiation-leukemia-virus-induced murine lymphoma by the biological response modifier polysaccharide K. Cancer Immunol Immunother. 41:389–396. 1995. View Article : Google Scholar

10 

Hattori ST, Komatsu N, Shichijo S and Itoh K: Protein-bound polysaccharide K induced apoptosis of the human Burkitt lymphoma cell line, Namalwa. Biomed Pharmacother. 58:226–230. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Jiménez-Medina E, Berruguilla E, Romero I, et al: The immunomodulator PSK induces in vitro cytotoxic activity in tumor cell lines via arrest of cell cycle and induction of apoptosis. BMC Cancer. 8:782008.PubMed/NCBI

12 

Thornberry NA: Caspases: key mediators of apoptosis. Chem Biol. 5:R97–R103. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Chen X, Lan X, Mo S, et al: p38 and ERK, but not JNK, are involved in copper-induced apoptosis in cultured cerebellar granule neurons. Biochem Res Commun. 379:944–948. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Reuter S, Eifes S, Dicato M, Aggarwal BB and Diederich M: Modulation of anti-apoptotic and survival pathways by curcumin as a strategy to induce apoptosis in cancer cells. Biochem Pharmacol. 76:1340–1351. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Cory S and Adams JM: The Bcl-2 family: regulators of the cellular life-or-death switch. Nat Res Cancer. 2:647–656. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Leibowitz B and Yu J: Mitochondrial signaling in cell death via the Bcl-2 family. Cancer Biol Ther. 9:417–422. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Xiao G, Fang H, Xing C and Xu W: Structure, function and inhibition of Bcl-2 family proteins: a new target for anti-tumor agents. Mini Rev Med Chem. 9:1596–1604. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Ahn YH, Jung JM and Hong SH: 8-chloro-cyclic AMP-induced growth inhibition and apoptosis is mediated by p38 mitogen-activated protein kinase activation in HL60 cells. Cancer Res. 65:4896–4901. 2005. View Article : Google Scholar

19 

Park SJ and Kim IS: The role of p38 MAPK activation in auranofin-induced apoptosis of human promyelocytic leukemia HL-60 cells. Br J Pharmacol. 146:506–513. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Torcia M, De Chiara G, Nencioni L, et al: Nerve growth factor inhibits apoptosis in memory B lymphocytes via inactivation of p38 MAPK, prevention of BCL-2 phosphorylation, and cytochrome release. J Biol Chem. 276:39027–39036. 2001. View Article : Google Scholar : PubMed/NCBI

21 

De Chiara G, Marcocci ME, Torcia M, et al: BCL-2 phosphorylation by p38 MAPK. J Biol Chem. 281:21353–21361. 2006.

22 

Riedl SJ and Salvesen GS: The apoptosome: signalling platform of cell death. Nat Rev Mol Cell Biol. 8:405–413. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Garcia A, Morales P, Arranz N, Delgado ME, Rafter J and Haza AI: Antiapoptotic effects of dietary antioxidants towards N-nitrosopiperidine and N-nitrosodibutylamine-induced apoptosis in HL-60 and HepG2 cells. J Appl Toxicol. 29:403–413. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Zhang R, Humphreys I, Sahu RP, Shi Y and Srivastava SK: In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway. Apoptosis. 13:1465–1478. 2008. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hirahara N, Edamatsu T, Fujieda A, Fujioka M, Wada T and Tajima Y: Protein-bound polysaccharide-K induces apoptosis via mitochondria and p38 mitogen‑activated protein kinase‑dependent pathways in HL‑60 promyelomonocytic leukemia cells. Oncol Rep 30: 99-104, 2013.
APA
Hirahara, N., Edamatsu, T., Fujieda, A., Fujioka, M., Wada, T., & Tajima, Y. (2013). Protein-bound polysaccharide-K induces apoptosis via mitochondria and p38 mitogen‑activated protein kinase‑dependent pathways in HL‑60 promyelomonocytic leukemia cells. Oncology Reports, 30, 99-104. https://doi.org/10.3892/or.2013.2412
MLA
Hirahara, N., Edamatsu, T., Fujieda, A., Fujioka, M., Wada, T., Tajima, Y."Protein-bound polysaccharide-K induces apoptosis via mitochondria and p38 mitogen‑activated protein kinase‑dependent pathways in HL‑60 promyelomonocytic leukemia cells". Oncology Reports 30.1 (2013): 99-104.
Chicago
Hirahara, N., Edamatsu, T., Fujieda, A., Fujioka, M., Wada, T., Tajima, Y."Protein-bound polysaccharide-K induces apoptosis via mitochondria and p38 mitogen‑activated protein kinase‑dependent pathways in HL‑60 promyelomonocytic leukemia cells". Oncology Reports 30, no. 1 (2013): 99-104. https://doi.org/10.3892/or.2013.2412
Copy and paste a formatted citation
x
Spandidos Publications style
Hirahara N, Edamatsu T, Fujieda A, Fujioka M, Wada T and Tajima Y: Protein-bound polysaccharide-K induces apoptosis via mitochondria and p38 mitogen‑activated protein kinase‑dependent pathways in HL‑60 promyelomonocytic leukemia cells. Oncol Rep 30: 99-104, 2013.
APA
Hirahara, N., Edamatsu, T., Fujieda, A., Fujioka, M., Wada, T., & Tajima, Y. (2013). Protein-bound polysaccharide-K induces apoptosis via mitochondria and p38 mitogen‑activated protein kinase‑dependent pathways in HL‑60 promyelomonocytic leukemia cells. Oncology Reports, 30, 99-104. https://doi.org/10.3892/or.2013.2412
MLA
Hirahara, N., Edamatsu, T., Fujieda, A., Fujioka, M., Wada, T., Tajima, Y."Protein-bound polysaccharide-K induces apoptosis via mitochondria and p38 mitogen‑activated protein kinase‑dependent pathways in HL‑60 promyelomonocytic leukemia cells". Oncology Reports 30.1 (2013): 99-104.
Chicago
Hirahara, N., Edamatsu, T., Fujieda, A., Fujioka, M., Wada, T., Tajima, Y."Protein-bound polysaccharide-K induces apoptosis via mitochondria and p38 mitogen‑activated protein kinase‑dependent pathways in HL‑60 promyelomonocytic leukemia cells". Oncology Reports 30, no. 1 (2013): 99-104. https://doi.org/10.3892/or.2013.2412
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team