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Article

Low-dose 1,25-dihydroxyvitamin D3 combined with arsenic trioxide synergistically inhibits proliferation of acute myeloid leukemia cells by promoting apoptosis

  • Authors:
    • Ji-Yeon Bae
    • Ji-Won Kim
    • Inho Kim
  • View Affiliations / Copyright

    Affiliations: Cancer Research Institute, Seoul National University, Seoul, Republic of Korea, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Pages: 485-491
    |
    Published online on: May 9, 2013
       https://doi.org/10.3892/or.2013.2444
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Abstract

Arsenic trioxide (As2O3) has shown substantial efficacy in the treatment of patients with acute promyelocytic leukemia, a specific subtype of acute myeloid leukemia (AML). However, since not all patients can achieve remission after treatment, it is necessary to develop a novel method to overcome this problem. We investigated the anti-leukemic effect of low-dose 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) in combination with As2O3 on the human AML cell lines HL-60 and K562. The cell viability was in reverse proportion to As2O3 or 1,25(OH)2D3 concentration. In both HL-60 and K562 cells, after the combination treatment with As2O3 and 1,25(OH)2D3 at a 10:1 ratio, the combination index (CI) values were <1 in all treatment groups. In the RT-PCR and western blot analysis, the combination treatment decreased Bcl-2 expression and increased Bax and caspase-3 expression more prominently than the single treatment. In the flow cytometric analysis performed in HL-60 cells, the proportion of late apoptotic cells was 4.9% in the control, 30.0% in cells treated with 1.0 µM As2O3, 8.1% in cells treated with 100 nM 1,25(OH)2D3, and 64.3% in cells treated with 1.0 µM As2O3 plus 100 nM 1,25(OH)2D3. In conclusion, low-dose 1,25(OH)2D3 combined with As2O3 synergistically inhibited proliferation of HL-60 and K562 cells. In addition, this combination activated the apoptosis pathway more prominently than the single‑drug treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Bae J, Kim J and Kim I: Low-dose 1,25-dihydroxyvitamin D3 combined with arsenic trioxide synergistically inhibits proliferation of acute myeloid leukemia cells by promoting apoptosis. Oncol Rep 30: 485-491, 2013.
APA
Bae, J., Kim, J., & Kim, I. (2013). Low-dose 1,25-dihydroxyvitamin D3 combined with arsenic trioxide synergistically inhibits proliferation of acute myeloid leukemia cells by promoting apoptosis. Oncology Reports, 30, 485-491. https://doi.org/10.3892/or.2013.2444
MLA
Bae, J., Kim, J., Kim, I."Low-dose 1,25-dihydroxyvitamin D3 combined with arsenic trioxide synergistically inhibits proliferation of acute myeloid leukemia cells by promoting apoptosis". Oncology Reports 30.1 (2013): 485-491.
Chicago
Bae, J., Kim, J., Kim, I."Low-dose 1,25-dihydroxyvitamin D3 combined with arsenic trioxide synergistically inhibits proliferation of acute myeloid leukemia cells by promoting apoptosis". Oncology Reports 30, no. 1 (2013): 485-491. https://doi.org/10.3892/or.2013.2444
Copy and paste a formatted citation
x
Spandidos Publications style
Bae J, Kim J and Kim I: Low-dose 1,25-dihydroxyvitamin D3 combined with arsenic trioxide synergistically inhibits proliferation of acute myeloid leukemia cells by promoting apoptosis. Oncol Rep 30: 485-491, 2013.
APA
Bae, J., Kim, J., & Kim, I. (2013). Low-dose 1,25-dihydroxyvitamin D3 combined with arsenic trioxide synergistically inhibits proliferation of acute myeloid leukemia cells by promoting apoptosis. Oncology Reports, 30, 485-491. https://doi.org/10.3892/or.2013.2444
MLA
Bae, J., Kim, J., Kim, I."Low-dose 1,25-dihydroxyvitamin D3 combined with arsenic trioxide synergistically inhibits proliferation of acute myeloid leukemia cells by promoting apoptosis". Oncology Reports 30.1 (2013): 485-491.
Chicago
Bae, J., Kim, J., Kim, I."Low-dose 1,25-dihydroxyvitamin D3 combined with arsenic trioxide synergistically inhibits proliferation of acute myeloid leukemia cells by promoting apoptosis". Oncology Reports 30, no. 1 (2013): 485-491. https://doi.org/10.3892/or.2013.2444
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