miR-573 regulates melanoma progression by targeting the melanoma cell adhesion molecule

Wang,He-Fei; Chen,Hong; Ma,Min-Wang; Wang,Ji-An; Tang,Ting-Ting; Ni,Lai-Sheng; Yu,Jin-Ling; Li,Yu-Zhen; Bai,Bing-Xue

doi:10.3892/or.2013.2451

July 2013 Volume 30 Issue 1

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July 2013 Volume 30 Issue 1

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Article

miR-573 regulates melanoma progression by targeting the melanoma cell adhesion molecule

Authors:
- He-Fei Wang
- Hong Chen
- Min-Wang Ma
- Ji-An Wang
- Ting-Ting Tang
- Lai-Sheng Ni
- Jin-Ling Yu
- Yu-Zhen Li
- Bing-Xue Bai
View Affiliations / Copyright

Affiliations: Department of Dermatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Pediatrics, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China, Department of Ophthalmology, The Affiliated Hospital of Medical College of Chinese People's Armed Police Forces, Tianjin 300162, P.R. China, Obstetrics and Gynecology Hospital, Harbin, Heilongjiang 150300, P.R. China, Orthopedic Surgery of Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Normal Surgical Department of the People's Hospital of Acheng City, Harbin, Heilongjiang 150300, P.R. China, Pathology of General Hospital of Jixi Mining Group, Jixi, Heilongjiang 158100, P.R. China
Pages: 520-526
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Published online on: May 13, 2013

https://doi.org/10.3892/or.2013.2451
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Abstract

Melanoma is a malignant tumor of the melanocytes. microRNAs (miRNAs) are emerging as important regulators of cancer-related processes. A thorough understanding of miRNAs in melanoma progression is important for developing new therapeutic targets. miRNA expression was detected by quantitative PCR. In vitro, MTT assay, colony formation assay, invasion assay and flow cytometry analysis were performed to test the effect of miR-573 on melanoma cells. The effect of miR-573 in vivo was validated using a murine xenograft model. Using quantitative PCR, we found that the expression levels of miR-573 were lower in melanoma tissues and cell lines compared to normal skin tissues. miR-573 upregulation inhibited melanoma cell proliferation and invasion, and overexpression of melanoma cell adhesion molecule (MCAM) could alleviate the effect of miR-573 on melanoma cells. In vivo, miR-573 overexpression groups showed lower rates of tumor growth compared with the control group. In conclusion, our results demonstrate that the elevated MCAM expression due to miR-573 reduction is essential in melanoma initiation and progression.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

miR-573 regulates melanoma progression by targeting the melanoma cell adhesion molecule

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