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Article

microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells

  • Authors:
    • Yeqing Mao
    • Hong Chen
    • Yiwei Lin
    • Xin Xu
    • Zhenghui Hu
    • Yi Zhu
    • Jian  Wu
    • Xianglai Xu
    • Xiangyi Zheng
    • Liping Xie
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
  • Pages: 327-333
    |
    Published online on: May 13, 2013
       https://doi.org/10.3892/or.2013.2452
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Abstract

microRNAs (miRNAs), small non-coding RNAs, have emerged as key regulators of a large number of genes. The present study aimed to explore novel biological functions of miR-330 in the human prostate cancer cell lines DU145 and PC3. We confirmed that miR-330 was downregulated and inversely correlated with specificity protein 1 (Sp1) expression. Overexpression of miR-330 by transfection of a chemically synthesized miR-330 mimic induced a reduction in expression levels of the Sp1 protein, accompanied by significant suppression of cellular migration and invasion capability. In addition, the Sp1-knockdown experiments presented similar phenomena. Finally, the luciferase reporter assay validated Sp1 as the direct target of miR-330. These findings indicate that miR-330 acts as an anti-metastatic miRNA in prostate cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Mao Y, Chen H, Lin Y, Xu X, Hu Z, Zhu Y, Wu J, Xu X, Zheng X, Xie L, Xie L, et al: microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells. Oncol Rep 30: 327-333, 2013.
APA
Mao, Y., Chen, H., Lin, Y., Xu, X., Hu, Z., Zhu, Y. ... Xie, L. (2013). microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells. Oncology Reports, 30, 327-333. https://doi.org/10.3892/or.2013.2452
MLA
Mao, Y., Chen, H., Lin, Y., Xu, X., Hu, Z., Zhu, Y., Wu, J., Xu, X., Zheng, X., Xie, L."microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells". Oncology Reports 30.1 (2013): 327-333.
Chicago
Mao, Y., Chen, H., Lin, Y., Xu, X., Hu, Z., Zhu, Y., Wu, J., Xu, X., Zheng, X., Xie, L."microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells". Oncology Reports 30, no. 1 (2013): 327-333. https://doi.org/10.3892/or.2013.2452
Copy and paste a formatted citation
x
Spandidos Publications style
Mao Y, Chen H, Lin Y, Xu X, Hu Z, Zhu Y, Wu J, Xu X, Zheng X, Xie L, Xie L, et al: microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells. Oncol Rep 30: 327-333, 2013.
APA
Mao, Y., Chen, H., Lin, Y., Xu, X., Hu, Z., Zhu, Y. ... Xie, L. (2013). microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells. Oncology Reports, 30, 327-333. https://doi.org/10.3892/or.2013.2452
MLA
Mao, Y., Chen, H., Lin, Y., Xu, X., Hu, Z., Zhu, Y., Wu, J., Xu, X., Zheng, X., Xie, L."microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells". Oncology Reports 30.1 (2013): 327-333.
Chicago
Mao, Y., Chen, H., Lin, Y., Xu, X., Hu, Z., Zhu, Y., Wu, J., Xu, X., Zheng, X., Xie, L."microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells". Oncology Reports 30, no. 1 (2013): 327-333. https://doi.org/10.3892/or.2013.2452
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