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Article

miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis

  • Authors:
    • De-Tao Wang
    • Zhong-Liang Ma
    • Yan-Li Li
    • Yue-Qing Wang
    • Bo-Tao Zhao
    • Jia-Li Wei
    • Xiang Qi
    • Xin-Tai Zhao
    • You-Xin Jin
  • View Affiliations / Copyright

    Affiliations: School of Life Sciences, Shanghai University, Shanghai 200444, P.R. China, Shanghai Shines Pharmaceuticals Co., Ltd., Shanghai 200032, P.R. China
  • Pages: 492-498
    |
    Published online on: May 13, 2013
       https://doi.org/10.3892/or.2013.2453
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Abstract

microRNAs (miRNAs) are a class of non-coding small RNAs that act as negative regulators of gene expression by binding to the 3'-untranslated region (3'-UTR) of target mRNAs. Tumor protein p53, a transcriptional factor, plays an important role in the progression of tumorigenesis. miR-150 was the only miRNA predicted to target 3'-UTR of p53 by Targetscan. In order to investigate the function of miR-150, p53 and relevant miRNAs in non-small cell lung cancer (NSCLC), we constructed two expression vectors of p53 (pcDNA3.1-p53 and pcDNA3.1-p53-3'-UTR) and two report vectors (pGL3-p53-3'-UTR and pGL3-p53-3'-mUTR). The activity of luciferase transfected with miR-150 mimics was lower by 30% when compared to that of the miRNA-negative control (miRNA-NC). Moreover, the p53 protein was downregulated by at least 50% when miR-150 mimics were cotransfected with pcDNA3.1-p53-3'-UTR when compared to miRNA-NC. We also determined the expression of miR-150 and p53 in NSCLC patient tissue samples. The expression of miR-150 in T2 stage tissue samples was higher than that in T1 stage tissue samples. The corresponding target gene p53 was correlated with miR-150 expression. In the present study, we further analyzed the cell cycle distribution. The cells transfected with pcDNA3.1-p53 were significantly arrested in the G1 phase when compared to the control cells. When miR-150 mimics were cotransfected with pcDNA3.1-p53-3'-UTR, the percentage of cells in the G1 phase was significantly lower by 4% when compared to miRNA-NC. To identify miRNAs that are regulated by the p53 protein, qRT-PCR was performed after pcDNA3.1-p53 transfection. miR-34a, miR-184, miR-181a and miR-148 were upregulated significantly. However, there was no distinct difference in the expression of miR-10a, miR-182 and miR-34c. Our results showed that miR-150 targets the 3'-UTR of p53, and p53 protein promotes the expression of miRNAs which affect cell cycle progression. These findings suggest that miR-150, p53 protein and relevant miRNAs are members of a regulatory network in NSCLC tumorigenesis.
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1 

Zabaleta J: MicroRNA: a bridge from H. pylori infection to gastritis and gastric cancer development. Front Genet. 3:2942012.PubMed/NCBI

2 

Harquail J, Benzina S and Robichaud GA: MicroRNAs and breast cancer malignancy: an overview of miRNA-regulated cancer processes leading to metastasis. Cancer Biomark. 11:269–280. 2012.PubMed/NCBI

3 

Wang F, Sun GP, Zou YF, Hao JQ, Zhong F and Ren WJ: MicroRNAs as promising biomarkers for gastric cancer. Cancer Biomark. 11:259–267. 2012.PubMed/NCBI

4 

Odjele A, Charest D and Morin PJ: miRNAs as important drivers of glioblastomas: a no-brainer? Cancer Biomark. 11:245–252. 2012.PubMed/NCBI

5 

Eldem V, Celikkol Akcay U, Ozhuner E, Bakir Y, Uranbey S and Unver T: Genome-wide identification of miRNAs responsive to drought in peach (Prunus persica) by high-throughput deep sequencing. PloS One. 7:e502982012. View Article : Google Scholar : PubMed/NCBI

6 

Endo H, Muramatsu T, Furuta M, et al: Potential of tumor-suppressive miR-596 targeting LGALS3BP as a therapeutic agent in oral cancer. Carcinogenesis. 34:560–569. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Li KK, Pang JC, Lau KM, et al: miR-383 is downregulated in medulloblastoma and targets peroxiredoxin 3 (PRDX3). Brain Pathol. Dec 11–2012.(Epub ahead of print). View Article : Google Scholar

8 

Masuda M, Miki Y, Hata S, et al: An induction of microRNA, miR-7 through estrogen treatment in breast carcinoma. J Transl Med. 10(Suppl 1): S22012. View Article : Google Scholar : PubMed/NCBI

9 

Novello C, Pazzaglia L, Cingolani C, et al: miRNA expression profile in human osteosarcoma: role of miR-1 and miR-133b in proliferation and cell cycle control. Int J Oncol. 42:667–675. 2013.PubMed/NCBI

10 

Ando H, Okamoto A, Yokota M, et al: Development of miR-92a delivery system for antiangiogenesis-based cancer therapy. J Gene Med. 15:20–27. 2012. View Article : Google Scholar

11 

Teixeira AL, Gomes M and Medeiros R: EGFR signaling pathway and related-miRNAs in age-related diseases: the example of miR-221 and miR-222. Front Genet. 3:2862012. View Article : Google Scholar : PubMed/NCBI

12 

Boldrup L, Coates PJ, Wahlgren M, Laurell G and Nylander K: Subsite-based alterations in miR-21, miR-125b, and miR-203 in squamous cell carcinoma of the oral cavity and correlation to important target proteins. J Carcinog. 11:182012. View Article : Google Scholar : PubMed/NCBI

13 

Hirata H, Ueno K, Shahryari V, et al: Oncogenic miRNA-182–5p targets Smad4 and RECK in human bladder cancer. PloS One. 7:e510562012.PubMed/NCBI

14 

Hassan F, Nuovo GJ, Crawford M, et al: MiR-101 and miR-144 regulate the expression of the CFTR chloride channel in the lung. PloS One. 7:e508372012. View Article : Google Scholar : PubMed/NCBI

15 

Sasaki A, Udaka Y, Tsunoda Y, et al: Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines. Anticancer Res. 32:4709–4713. 2012.PubMed/NCBI

16 

Suzuki HI and Miyazono K: p53 actions on microRNA expression and maturation pathway. Methods Mol Biol. 962:165–181. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Gurtler A, Kunz N, Gomolka M, et al: Stain-Free technology as a normalization tool in Western blot analysis. Anal Biochem. 433:105–111. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Hirano S: Western blot analysis. Methods Mol Biol. 926:87–97. 2012. View Article : Google Scholar

19 

Kao CH, Cheng CM, Chuang KH, et al: A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis. Anal Biochem. 431:1–3. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Nybo K: Molecular biology techniques Q&A. Western blot: protein migration. Biotechniques. 53:23–24. 2012.

21 

Li XJ, Ji MH, Zhong SL, et al: MicroRNA-34a modulates chemosensitivity of breast cancer cells to adriamycin by targeting Notch1. Arch Med Res. 43:514–521. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Siemens H, Neumann J, Jackstadt R, et al: Detection of miR-34a promoter methylation in combination with elevated expression of c-Met and β-catenin predicts distant metastasis of colon cancer. Clin Cancer Res. 9:710–720. 2012.PubMed/NCBI

23 

Zhang HS, Chen XY, Wu TC, Sang WW and Ruan Z: MiR-34a is involved in Tat-induced HIV-1 long terminal repeat (LTR) transactivation through the SIRT1/NFkappaB pathway. FEBS Lett. 586:4203–4207. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Candas D, Fan M, Nantajit D, et al: CyclinB1/Cdk1 phosphorylates mitochondrial antioxidant MnSOD in cell adaptive response to radiation stress. J Mol Cell Biol. Jan 31–2013.(Epub ahead of print).

25 

Haflidadottir BS, Bergsteinsdottir K, Praetorius C and Steingrimsson E: miR-148 regulates Mitf in melanoma cells. PloS One. 5:e115742010. View Article : Google Scholar : PubMed/NCBI

26 

Iovino N, Pane A and Gaul U: miR-184 has multiple roles in Drosophila female germline development. Dev Cell. 17:123–133. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Liu C, Teng ZQ, Santistevan NJ, et al: Epigenetic regulation of miR-184 by MBD1 governs neural stem cell proliferation and differentiation. Cell Stem Cell. 6:433–444. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Chen Y, Tsai YH and Tseng SH: Inhibition of cyclin-dependent kinase 1-induced cell death in neuroblastoma cells through the microRNA-34a-MYCN-survivin pathway. Surgery. 153:4–16. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Zhang C, Yao Z, Zhu M, et al: Inhibitory effects of microRNA-34a on cell migration and invasion of invasive urothelial bladder carcinoma by targeting Notch1. J Huazhong Univ Sci Technolog Med Sci. 32:375–382. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Venta R, Valk E, Koivomagi M and Loog M: Double-negative feedback between S-phase cyclin-CDK and CKI generates abruptness in the G1/S switch. Front Physiol. 3:4592012. View Article : Google Scholar : PubMed/NCBI

31 

Nagle AA, Gan FF, Jones G, So CL, Wells G and Chew EH: Induction of tumor cell death through targeting tubulin and evoking dysregulation of cell cycle regulatory proteins by multifunctional cinnamaldehydes. PloS One. 7:e501252012. View Article : Google Scholar : PubMed/NCBI

32 

Chow JP and Poon RY: The CDK1 inhibitory kinase MYT1 in DNA damage checkpoint recovery. Oncogene. Nov 12–2012.(Epub ahead of print).

33 

Guarnieri V, Battista C, Muscarella LA, et al: CDC73 mutations and parafibromin immunohistochemistry in parathyroid tumors: clinical correlations in a single-centre patient cohort. Cell Oncol (Dordr). 35:411–422. 2012. View Article : Google Scholar

34 

Amrich CG, Davis CP, Rogal WP, et al: Cdc73 subunit of Paf1 complex contains C-terminal Ras-like domain that promotes association of Paf1 complex with chromatin. J Biol Chem. 287:10863–10875. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Zhang JH, Seigneur EM, Pandey M, et al: The EIF4EBP3 translational repressor is a marker of CDC73 tumor suppressor haploinsufficiency in a parathyroid cancer syndrome. Cell Death Dis. 3:2662012. View Article : Google Scholar : PubMed/NCBI

36 

Masi G, Barzon L, Iacobone M, et al: Clinical, genetic, and histopathologic investigation of CDC73-related familial hyperparathyroidism. Endocr Relat Cancer. 15:1115–1126. 2008. View Article : Google Scholar : PubMed/NCBI

37 

Jiang Y, Zhang XY, Sun L, et al: Methyl methanesulfonate induces apoptosis in p53-deficient H1299 and Hep3B cells through a caspase 2- and mitochondria-associated pathway. Environ Toxicol Pharmacol. 34:694–704. 2012. View Article : Google Scholar : PubMed/NCBI

38 

Debernardi S, Skoulakis S, Molloy G, Chaplin T, Dixon-McIver A and Young BD: MicroRNA miR-181a correlates with morphological sub-class of acute myeloid leukaemia and the expression of its target genes in global genome-wide analysis. Leukemia. 21:912–916. 2007.PubMed/NCBI

39 

Shin KH, Bae SD, Hong HS, Kim RH, Kang MK and Park NH: miR-181a shows tumor suppressive effect against oral squamous cell carcinoma cells by downregulating K-ras. Biochem Biophys Res Commun. 404:896–902. 2011. View Article : Google Scholar : PubMed/NCBI

40 

Xie W, Li Z, Li M, Xu N and Zhang Y: miR-181a and inflammation: miRNA homeostasis response to inflammatory stimuli in vivo. Biochem Biophys Res Commun. 430:647–652. 2012. View Article : Google Scholar : PubMed/NCBI

41 

Kashat M, Azzouz L, Sarkar SH, Kong D, Li Y and Sarkar FH: Inactivation of AR and Notch-1 signaling by miR-34a attenuates prostate cancer aggressiveness. Am J Transl Res. 4:432–442. 2012.PubMed/NCBI

42 

Xia J, Duan Q, Ahmad A, et al: Genistein inhibits cell growth and induces apoptosis through up-regulation of miR-34a in pancreatic cancer cells. Curr Drug Targets. 13:1750–1756. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang D, Ma Z, Li Y, Wang Y, Zhao B, Wei J, Qi X, Zhao X and Jin Y: miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis. Oncol Rep 30: 492-498, 2013.
APA
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J. ... Jin, Y. (2013). miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis. Oncology Reports, 30, 492-498. https://doi.org/10.3892/or.2013.2453
MLA
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J., Qi, X., Zhao, X., Jin, Y."miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis". Oncology Reports 30.1 (2013): 492-498.
Chicago
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J., Qi, X., Zhao, X., Jin, Y."miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis". Oncology Reports 30, no. 1 (2013): 492-498. https://doi.org/10.3892/or.2013.2453
Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Ma Z, Li Y, Wang Y, Zhao B, Wei J, Qi X, Zhao X and Jin Y: miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis. Oncol Rep 30: 492-498, 2013.
APA
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J. ... Jin, Y. (2013). miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis. Oncology Reports, 30, 492-498. https://doi.org/10.3892/or.2013.2453
MLA
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J., Qi, X., Zhao, X., Jin, Y."miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis". Oncology Reports 30.1 (2013): 492-498.
Chicago
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J., Qi, X., Zhao, X., Jin, Y."miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis". Oncology Reports 30, no. 1 (2013): 492-498. https://doi.org/10.3892/or.2013.2453
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