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miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis

  • Authors:
    • De-Tao Wang
    • Zhong-Liang Ma
    • Yan-Li Li
    • Yue-Qing Wang
    • Bo-Tao Zhao
    • Jia-Li Wei
    • Xiang Qi
    • Xin-Tai Zhao
    • You-Xin Jin

  • View Affiliations

  • Published online on: May 13, 2013     https://doi.org/10.3892/or.2013.2453
  • Pages: 492-498
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Abstract

microRNAs (miRNAs) are a class of non-coding small RNAs that act as negative regulators of gene expression by binding to the 3'-untranslated region (3'-UTR) of target mRNAs. Tumor protein p53, a transcriptional factor, plays an important role in the progression of tumorigenesis. miR-150 was the only miRNA predicted to target 3'-UTR of p53 by Targetscan. In order to investigate the function of miR-150, p53 and relevant miRNAs in non-small cell lung cancer (NSCLC), we constructed two expression vectors of p53 (pcDNA3.1-p53 and pcDNA3.1-p53-3'-UTR) and two report vectors (pGL3-p53-3'-UTR and pGL3-p53-3'-mUTR). The activity of luciferase transfected with miR-150 mimics was lower by 30% when compared to that of the miRNA-negative control (miRNA-NC). Moreover, the p53 protein was downregulated by at least 50% when miR-150 mimics were cotransfected with pcDNA3.1-p53-3'-UTR when compared to miRNA-NC. We also determined the expression of miR-150 and p53 in NSCLC patient tissue samples. The expression of miR-150 in T2 stage tissue samples was higher than that in T1 stage tissue samples. The corresponding target gene p53 was correlated with miR-150 expression. In the present study, we further analyzed the cell cycle distribution. The cells transfected with pcDNA3.1-p53 were significantly arrested in the G1 phase when compared to the control cells. When miR-150 mimics were cotransfected with pcDNA3.1-p53-3'-UTR, the percentage of cells in the G1 phase was significantly lower by 4% when compared to miRNA-NC. To identify miRNAs that are regulated by the p53 protein, qRT-PCR was performed after pcDNA3.1-p53 transfection. miR-34a, miR-184, miR-181a and miR-148 were upregulated significantly. However, there was no distinct difference in the expression of miR-10a, miR-182 and miR-34c. Our results showed that miR-150 targets the 3'-UTR of p53, and p53 protein promotes the expression of miRNAs which affect cell cycle progression. These findings suggest that miR-150, p53 protein and relevant miRNAs are members of a regulatory network in NSCLC tumorigenesis.
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July 2013
Volume 30 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Journal Metrics
  • Impact Factor: 4.2
  • Ranked Oncology
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  • CiteScore: 8.4
  • CiteScore Rank: Q1: #68/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.786
  • SCImago Journal Rank (SJR): 0.812
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Spandidos Publications style
Wang D, Ma Z, Li Y, Wang Y, Zhao B, Wei J, Qi X, Zhao X and Jin Y: miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis. Oncol Rep 30: 492-498, 2013
APA
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J. ... Jin, Y. (2013). miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis. Oncology Reports, 30, 492-498. https://doi.org/10.3892/or.2013.2453
MLA
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J., Qi, X., Zhao, X., Jin, Y."miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis". Oncology Reports 30.1 (2013): 492-498.
Chicago
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J., Qi, X., Zhao, X., Jin, Y."miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis". Oncology Reports 30, no. 1 (2013): 492-498. https://doi.org/10.3892/or.2013.2453