A new curcumin analogue exhibits enhanced antitumor activity in nasopharyngeal carcinoma

Pan,Yunbao; Xiao,Jian; Liang,Guang; Wang,Mengyao; Wang,Dujuan; Wang,Sumei; Yang,Huiling

doi:10.3892/or.2013.2457

A new curcumin analogue exhibits enhanced antitumor activity in nasopharyngeal carcinoma

Authors:
- Yunbao Pan
- Jian Xiao
- Guang Liang
- Mengyao Wang
- Dujuan Wang
- Sumei Wang
- Huiling Yang
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Affiliations: Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China, School of Pharmacy, Wenzhou Medical College, Wenzhou, Zhejiang 325025, P.R. China
Published online on: May 14, 2013 https://doi.org/10.3892/or.2013.2457
Pages: 239-245

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Abstract

The aim of the present study was to evaluate the antitumor effects of the curcumin analogue GL63 on radioresistant nasopharyngeal carcinoma (NPC) CNE2R cells and parental CNE2 cells. The cell viability and proliferation of NPC cells were detected by MTT assay and colony formation assay. The suppressive effect on tumor growth was examined using in vivo subcutaneously inoculated NPC tumor models using nude mice. The cell cycle distribution was detected using flow cytometry. Apoptosis was examined by Hoechst 33342 and Annexin V/PI staining assay. The protein expression of endoplasmic reticulum (ER) stress pathway markers, XBP-1, ATF-4 and CHOP, were examined by western blotting. A growth inhibitory effect was observed following treatment with GL63 in a dose-dependent manner and was more potent when compared to curcumin. GL63 at 5 µM induced significant G2/M arrest and apoptosis in NPC. The tumor-suppressive activity of GL63 in NPC xenograft models was more potent when compared to curcumin. Furthermore, GL63 induced an ER stress response, upregulation of CHOP, XBP-1 and ATF-4 expression, while the same concentration of curcumin had no effect on ER stress. These results suggest that GL63 has more potent antitumor activity than curcumin, which is associated with activation of ER stress, induction of G2/M arrest and apoptosis in NPC cells.

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