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July 2013 Volume 30 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

Silencing of pancreatic adenocarcinoma upregulated factor by RNA interference inhibits the malignant phenotypes of human colorectal cancer cells

  • Authors:
    • Peng-Fei Liu
    • Yun-Yun Wu
    • You Hu
    • Lei Wang
    • Song-Bing He
    • Yi-Bei Zhu
    • Xin-Guo Zhu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Immunology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
  • Pages: 213-220
    |
    Published online on: May 15, 2013
       https://doi.org/10.3892/or.2013.2478
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Abstract

Aberrant expression of pancreatic adenocarcinoma upregulated factor (PAUF), a novel secretory protein, has been reported in several types of cancer. However, in colorectal cancer (CRC), whether PAUF also plays its oncogenic role through the Wnt/β-catenin pathway and its effect in regulating malignant phenotypes of CRC is unknown. In this study, we detected PAUF and β-catenin expression levels by immunohistochemical analysis and real-time PCR in CRC tissues, adjacent non-tumor tissues (NATs) and 5 CRC cell lines. The results demonstrated that the expression of PAUF and β-catenin in tumor tissues was higher than in NATs. Moreover, the expression of PAUF was correlated with the expression of β-catenin in both tumor tissues and NATs. The HCT116 cell line, which has the highest PAUF expression of the 5 cell lines, was transfected with small interfering RNA (siRNA) targeting on PAUF, which significantly downregulated the expression of PAUF in cancer cells. Successful transfection was confirmed by using RT-PCR and western blot analysis. Further studies demonstrated that PAUF-siRNA inhibited the proliferation of CRC cells, promoted their apoptosis and induced G0/G1 cell cycle arrest. At the same time, PAUF-siRNA inhibited the invasion, adhesion and migration of the tumor cells. In conclusion, this study suggested that PAUF was expressed in CRC at a high frequency. Interference of PAUF may be an effective strategy for regulating malignant phenotypes of CRC through the Wnt/β-catenin pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Liu P, Wu Y, Hu Y, Wang L, He S, Zhu Y and Zhu X: Silencing of pancreatic adenocarcinoma upregulated factor by RNA interference inhibits the malignant phenotypes of human colorectal cancer cells. Oncol Rep 30: 213-220, 2013.
APA
Liu, P., Wu, Y., Hu, Y., Wang, L., He, S., Zhu, Y., & Zhu, X. (2013). Silencing of pancreatic adenocarcinoma upregulated factor by RNA interference inhibits the malignant phenotypes of human colorectal cancer cells. Oncology Reports, 30, 213-220. https://doi.org/10.3892/or.2013.2478
MLA
Liu, P., Wu, Y., Hu, Y., Wang, L., He, S., Zhu, Y., Zhu, X."Silencing of pancreatic adenocarcinoma upregulated factor by RNA interference inhibits the malignant phenotypes of human colorectal cancer cells". Oncology Reports 30.1 (2013): 213-220.
Chicago
Liu, P., Wu, Y., Hu, Y., Wang, L., He, S., Zhu, Y., Zhu, X."Silencing of pancreatic adenocarcinoma upregulated factor by RNA interference inhibits the malignant phenotypes of human colorectal cancer cells". Oncology Reports 30, no. 1 (2013): 213-220. https://doi.org/10.3892/or.2013.2478
Copy and paste a formatted citation
x
Spandidos Publications style
Liu P, Wu Y, Hu Y, Wang L, He S, Zhu Y and Zhu X: Silencing of pancreatic adenocarcinoma upregulated factor by RNA interference inhibits the malignant phenotypes of human colorectal cancer cells. Oncol Rep 30: 213-220, 2013.
APA
Liu, P., Wu, Y., Hu, Y., Wang, L., He, S., Zhu, Y., & Zhu, X. (2013). Silencing of pancreatic adenocarcinoma upregulated factor by RNA interference inhibits the malignant phenotypes of human colorectal cancer cells. Oncology Reports, 30, 213-220. https://doi.org/10.3892/or.2013.2478
MLA
Liu, P., Wu, Y., Hu, Y., Wang, L., He, S., Zhu, Y., Zhu, X."Silencing of pancreatic adenocarcinoma upregulated factor by RNA interference inhibits the malignant phenotypes of human colorectal cancer cells". Oncology Reports 30.1 (2013): 213-220.
Chicago
Liu, P., Wu, Y., Hu, Y., Wang, L., He, S., Zhu, Y., Zhu, X."Silencing of pancreatic adenocarcinoma upregulated factor by RNA interference inhibits the malignant phenotypes of human colorectal cancer cells". Oncology Reports 30, no. 1 (2013): 213-220. https://doi.org/10.3892/or.2013.2478
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