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Article

MDA-7/IL-24 suppresses tumor adhesion and invasive potential in hepatocellular carcinoma cell lines

  • Authors:
    • Wei Huo
    • Zhi-Min Li
    • Xiao-Min Zhu
    • Yong-Ming Bao
    • Li-Jia An
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, Dalian Municipal Central Hospital, Dalian, Liaoning 116033, P.R. China, School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, P.R. China
  • Pages: 986-992
    |
    Published online on: May 29, 2013
       https://doi.org/10.3892/or.2013.2507
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Abstract

Melanoma differentiation associated gene-7 (MDA-7)/interleukin‑24 (IL-24) has been considered as a tumor-suppressor gene, which suppresses the growth and induces the apoptosis of cancer cells. In the present study, we investigated the effect and mechanisms of MDA-7/IL-24 regarding the inhibition of metastasis of HepG2 and BEL-7402 human hepatocellular carcinoma (HCC) cells in vitro. We established MDA-7/IL-24-overexpressing HepG2 and BEL-7402 cell lines and found that MDA-7/IL-24 overexpression inhibited tumor cell adhesion and invasion, and induced G2/M arrest in tumor cells. To explore its mechanism of action, western blotting and real-time-PCR assay were used to investigate the expression of E-cadherin, CD44, ICAM-1, matrix metalloproteinase (MMP)-2 and -9, CyclinB, Twist, survivin, p-ERK and p-Akt. ELISA assay was used to measure the secretion of TGF-β, and a reporter gene assay was used to detected the transcriptional activity of NF-κB and AP-1 in HepG2 and BEL-7402 cells. The results showed that MDA-7/IL-24 overexpression decreased the expression of CD44, ICAM-1, MMP-2/-9, CyclinB, Twist, survivin, TGF-β and p-Akt, transcriptional activity of NF-κB, and increased the expression of E-cadherin and p-ERK and transcriptional activity of AP-1 in HepG2 and BEL-7402 cells. Our results revealed that MDA-7/IL-24 mediated the inhibition of adhesion and invasion in HepG2 and BEL-7402 cells by suppressing metastasis-related gene expression. Thus, MDA-7/IL-24 may be used as a novel cancer-suppressor gene for the therapy of human HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Huo W, Li Z, Zhu X, Bao Y and An L: MDA-7/IL-24 suppresses tumor adhesion and invasive potential in hepatocellular carcinoma cell lines. Oncol Rep 30: 986-992, 2013.
APA
Huo, W., Li, Z., Zhu, X., Bao, Y., & An, L. (2013). MDA-7/IL-24 suppresses tumor adhesion and invasive potential in hepatocellular carcinoma cell lines. Oncology Reports, 30, 986-992. https://doi.org/10.3892/or.2013.2507
MLA
Huo, W., Li, Z., Zhu, X., Bao, Y., An, L."MDA-7/IL-24 suppresses tumor adhesion and invasive potential in hepatocellular carcinoma cell lines". Oncology Reports 30.2 (2013): 986-992.
Chicago
Huo, W., Li, Z., Zhu, X., Bao, Y., An, L."MDA-7/IL-24 suppresses tumor adhesion and invasive potential in hepatocellular carcinoma cell lines". Oncology Reports 30, no. 2 (2013): 986-992. https://doi.org/10.3892/or.2013.2507
Copy and paste a formatted citation
x
Spandidos Publications style
Huo W, Li Z, Zhu X, Bao Y and An L: MDA-7/IL-24 suppresses tumor adhesion and invasive potential in hepatocellular carcinoma cell lines. Oncol Rep 30: 986-992, 2013.
APA
Huo, W., Li, Z., Zhu, X., Bao, Y., & An, L. (2013). MDA-7/IL-24 suppresses tumor adhesion and invasive potential in hepatocellular carcinoma cell lines. Oncology Reports, 30, 986-992. https://doi.org/10.3892/or.2013.2507
MLA
Huo, W., Li, Z., Zhu, X., Bao, Y., An, L."MDA-7/IL-24 suppresses tumor adhesion and invasive potential in hepatocellular carcinoma cell lines". Oncology Reports 30.2 (2013): 986-992.
Chicago
Huo, W., Li, Z., Zhu, X., Bao, Y., An, L."MDA-7/IL-24 suppresses tumor adhesion and invasive potential in hepatocellular carcinoma cell lines". Oncology Reports 30, no. 2 (2013): 986-992. https://doi.org/10.3892/or.2013.2507
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