CCL21 as an independent favorable prognostic factor for stage III/IV colorectal cancer

Zou,Yifeng; Chen,Yufeng; Wu,Xianrui; Yuan,Ruixue; Cai,Zerong; He,Xiaosheng; Fan,Xinjuan; Wang,Lei; Wu,Xiaojian; Lan,Ping

doi:10.3892/or.2013.2533

CCL21 as an independent favorable prognostic factor for stage III/IV colorectal cancer

Authors:
- Yifeng Zou
- Yufeng Chen
- Xianrui Wu
- Ruixue Yuan
- Zerong Cai
- Xiaosheng He
- Xinjuan Fan
- Lei Wang
- Xiaojian Wu
- Ping Lan
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Affiliations: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, P.R. China, Gastrointestinal Institute of Sun Yat-sen University, Guangzhou, Guangdong, P.R. China
Published online on: June 11, 2013 https://doi.org/10.3892/or.2013.2533
Pages: 659-666

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Abstract

The aim of the present study was to investigate the expression dynamics of CCL21 and its prognostic signiﬁcance in human stage III/IV colorectal cancer (CRC). CCL21 expression dynamics were detected with western blotting. The expression of CCL21 in CRC tissue microarrays was examined by immunohistochemistry. The optimal cut-point of CCL21 expression was assessed by the X-tile program. The prognostic significance was analyzed using both Kaplan-Meier curves and Cox regression analysis. Western blot analysis demonstrated that CCL21 expression was comparable in the CRC and normal colorectal tissues. According to the X-tile program, the cut-point for high expression of CCL21 in CRC was determined when the CCL21 expression index was >56.1. Overexpression of CCL21 was signiﬁcantly correlated with larger tumor diameter, more mucinous carcinoma or signet ring cell carcinoma and poor tumor differentiation. Patients with high expression of CCL21 had a higher overall survival rate in comparison to patients with low expression. In the multivariate Cox regression analysis, CCL21 expression was found to be an independent prognostic biomarker for CRC. ROC curves showed that CCL21 expression could improve the prognostic capability of TNM stage in stage III/IV CRC patients. High expression of CCL21 is an independent and useful biomarker for predicting longer survival of stage III/IV CRC patients.

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