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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

CtBP1 is involved in epithelial-mesenchymal transition and is a potential therapeutic target for hepatocellular carcinoma

  • Authors:
    • Xiao-Ling Zhang
    • Cheng-Xin Huang
    • Jie Zhang
    • Akira Inoue
    • Si-En Zeng
    • Sheng-Jun Xiao
  • View Affiliations / Copyright

    Affiliations: GuangXi Key Laboratory of Molecular Medicine in Liver Injury and Repair, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, P.R. China, Department of Pathology, Afilliated Hospital of Guilin Medical University, Guilin, Guangxi, P.R. China, Department of Otolaryngology, Osaka City University Graduate School of Medicine, Osaka, Japan, Department of Pathology, Guilin Medical University, Guilin, Guangxi, P.R. China
  • Pages: 809-814
    |
    Published online on: June 11, 2013
       https://doi.org/10.3892/or.2013.2537
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Abstract

Hepatocellular carcinoma (HCC) is a highly invasive type of cancer. Metastasis is the leading cause of mortality of advanced HCC patients. In the metastasis cascade, cancer cells undergo epithelial-mesenchymal transition resulting in the loss of cell‑to‑cell adhesion, migration and invasion into the stroma. Loss of E-cadherin expression is a key molecular event in epithelial-mesenchymal transition through several regulatory mechanisms including epigenetic modification, regulation by inhibitory transcriptional factors and deletion of chromosome 16q24 locus. C-terminal binding protein 1 (CtBP1) functions as a corepressor binding to several transcriptional factors and suppresses E-cadherin expression. We found that CtBP1 was upregulated in HCC when compared with paired normal liver tissues and was inversely correlated with E-cadherin expression in HCC by immunohistochemical assay using tissue array. Western blot analysis confirmed the results of the immunohistochemical assays. When CtBP1 was knocked down by siRNA in HepG2 cells (a human HCC cell line), E-cadherin was upregulated and the invasive ability of HepG2 cells was inhibited. In addition, following CtBP1 knockdown, the cell viability was decreased along with increased apoptosis rather than cell cycle arrest. These data suggest a pivotal role of CtBP1 in EMT of HCC, and its potential as a therapeutic target in human disease.
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1 

Clavien PA, Lesurtel M, Bossuyt PM, et al: Recommendations for liver transplantation for hepatocellular carcinoma: an international consensus conference report. Lancet Oncol. 13:e11–e22. 2012. View Article : Google Scholar : PubMed/NCBI

2 

El-Serag HB: Hepatocellular carcinoma. N Engl J Med. 365:1118–1127. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Schrader J, Gordon-Walker TT, Aucott RL, et al: Matrix stiffness modulates proliferation, chemotherapeutic response, and dormancy in hepatocellular carcinoma cells. Hepatology. 53:1192–1205. 2011. View Article : Google Scholar

4 

Osada T, Sakamoto M, Ino Y, et al: E-cadherin is involved in the intrahepatic metastasis of hepatocellular carcinoma. Hepatology. 24:1460–1467. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Wei Y, Van Nhieu JT, Prigent S, Srivatanakul P, Tiollais P and Buendia MA: Altered expression of E-cadherin in hepatocellular carcinoma: correlations with genetic alterations, β-catenin expression, and clinical features. Hepatology. 36:692–701. 2002.PubMed/NCBI

6 

Marsit CJ, Posner MR, McClean MD and Kelsey KT: Hypermethylation of E-cadherin is an independent predictor of improved survival in head and neck squamous cell carcinoma. Cancer. 113:1566–1571. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Maeda G, Chiba T, Aoba T and Imai K: Epigenetic inactivation of E-cadherin by promoter hypermethylation in oral carcinoma cells. Odontology. 95:24–29. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Jiao W, Miyazaki K and Kitajima Y: Inverse correlation between E-cadherin and Snail expression in hepatocellular carcinoma cell lines in vitro and in vivo. Br J Cancer. 86:98–101. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Evans AJ, Russell RC, Roche O, et al: VHL promotes E2 box-dependent E-cadherin transcription by HIF-mediated regulation of SIP1 and snail. Mol Cell Biol. 27:157–169. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Ohshima K, Haraoka S, Yoshioka S, et al: Chromosome 16q deletion and loss of E-cadherin expression in Hodgkin and Reed-Sternberg cells. Int J Cancer. 92:678–682. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Pan Y, Matsuyama H, Wang N, et al: Chromosome 16q24 deletion and decreased E-cadherin expression: possible association with metastatic potential in prostate cancer. Prostate. 36:31–38. 1998. View Article : Google Scholar : PubMed/NCBI

12 

Liu YC, Shen CY, Wu HS, et al: Mechanisms inactivating the gene for E-cadherin in sporadic gastric carcinomas. World J Gastroenterol. 12:2168–2173. 2006.PubMed/NCBI

13 

Deng Y, Deng H, Liu J, et al: Transcriptional down-regulation of Brca1 and E-cadherin by CtBP1 in breast cancer. Mol Carcinog. 51:500–507. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Moreno-Bueno G, Portillo F and Cano A: Transcriptional regulation of cell polarity in EMT and cancer. Oncogene. 27:6958–6969. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Pena C, Garcia JM, Garcia V, et al: The expression levels of the transcriptional regulators p300 and CtBP modulate the correlations between SNAIL, ZEB1, E-cadherin and vitamin D receptor in human colon carcinomas. Int J Cancer. 119:2098–2104. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Lu W, Dong J, Huang Z, Guo D, Liu Y and Shi S: Comparison of four current staging systems for Chinese patients with hepatocellular carcinoma undergoing curative resection: Okuda, CLIP, TNM and CUPI. J Gastroenterol Hepatol. 23:1874–1878. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Gotte M, Kersting C, Radke I, Kiesel L and Wulfing P: An expression signature of syndecan-1 (CD138), E-cadherin and c-met is associated with factors of angiogenesis and lymphangiogenesis in ductal breast carcinoma in situ. Breast Cancer Res. 9:R82007. View Article : Google Scholar : PubMed/NCBI

18 

Chinnadurai G: CtBP, an unconventional transcriptional corepressor in development and oncogenesis. Mol Cell. 9:213–224. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Schaeper U, Subramanian T, Lim L, Boyd JM and Chinnadurai G: Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular protein is disrupted by E1A through a conserved PLDLS motif. J Biol Chem. 273:8549–8552. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Zhao LJ, Subramanian T, Vijayalingam S and Chinnadurai G: PLDLS-dependent interaction of E1A with CtBP: regulation of CtBP nuclear localization and transcriptional functions. Oncogene. 26:7544–7551. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Frisch SM: Antioncogenic effect of adenovirus E1A in human tumor cells. Proc Natl Acad Sci USA. 88:9077–9081. 1991. View Article : Google Scholar : PubMed/NCBI

22 

Frisch SM: Tumor suppression activity of adenovirus E1a protein: anoikis and the epithelial phenotype. Adv Cancer Res. 80:39–49. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Grooteclaes ML and Frisch SM: Evidence for a function of CtBP in epithelial gene regulation and anoikis. Oncogene. 19:3823–3828. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Thiery JP: Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer. 2:442–454. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Kippenberger S, Loitsch S, Thaci D, et al: Restoration of E-cadherin sensitizes human melanoma cells for apoptosis. Melanoma Res. 16:393–403. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Lowy AM, Knight J and Groden J: Restoration of E-cadherin/β-catenin expression in pancreatic cancer cells inhibits growth by induction of apoptosis. Surgery. 132:141–148. 2002.

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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Huang C, Zhang J, Inoue A, Zeng S and Xiao S: CtBP1 is involved in epithelial-mesenchymal transition and is a potential therapeutic target for hepatocellular carcinoma. Oncol Rep 30: 809-814, 2013.
APA
Zhang, X., Huang, C., Zhang, J., Inoue, A., Zeng, S., & Xiao, S. (2013). CtBP1 is involved in epithelial-mesenchymal transition and is a potential therapeutic target for hepatocellular carcinoma. Oncology Reports, 30, 809-814. https://doi.org/10.3892/or.2013.2537
MLA
Zhang, X., Huang, C., Zhang, J., Inoue, A., Zeng, S., Xiao, S."CtBP1 is involved in epithelial-mesenchymal transition and is a potential therapeutic target for hepatocellular carcinoma". Oncology Reports 30.2 (2013): 809-814.
Chicago
Zhang, X., Huang, C., Zhang, J., Inoue, A., Zeng, S., Xiao, S."CtBP1 is involved in epithelial-mesenchymal transition and is a potential therapeutic target for hepatocellular carcinoma". Oncology Reports 30, no. 2 (2013): 809-814. https://doi.org/10.3892/or.2013.2537
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Huang C, Zhang J, Inoue A, Zeng S and Xiao S: CtBP1 is involved in epithelial-mesenchymal transition and is a potential therapeutic target for hepatocellular carcinoma. Oncol Rep 30: 809-814, 2013.
APA
Zhang, X., Huang, C., Zhang, J., Inoue, A., Zeng, S., & Xiao, S. (2013). CtBP1 is involved in epithelial-mesenchymal transition and is a potential therapeutic target for hepatocellular carcinoma. Oncology Reports, 30, 809-814. https://doi.org/10.3892/or.2013.2537
MLA
Zhang, X., Huang, C., Zhang, J., Inoue, A., Zeng, S., Xiao, S."CtBP1 is involved in epithelial-mesenchymal transition and is a potential therapeutic target for hepatocellular carcinoma". Oncology Reports 30.2 (2013): 809-814.
Chicago
Zhang, X., Huang, C., Zhang, J., Inoue, A., Zeng, S., Xiao, S."CtBP1 is involved in epithelial-mesenchymal transition and is a potential therapeutic target for hepatocellular carcinoma". Oncology Reports 30, no. 2 (2013): 809-814. https://doi.org/10.3892/or.2013.2537
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