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Article

Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway

  • Authors:
    • Joon Wοο Lee
    • Cheol Park
    • Min Hο Han
    • Su Hyun Hong
    • Tae Kyung Lee
    • Shin Hwa Lee
    • Gi-Young Kim
    • Yung Hyun Choi
  • View Affiliations / Copyright

    Affiliations: Bunpo School, Nam-gu, Busan 608-832, Republic of Korea, Department of Molecular Biology, College of Natural Sciences, Dongeui University, Busan 614-714, Republic of Korea, Anti-Aging Research Center and Blue-Bio Industry RIC, Dongeui University, Busan 614-714, Republic of Korea, Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan 614‑052, Republic of Korea, Department of Biology, New York University, New York, NY 10012, USA, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea, Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea
  • Pages: 1231-1238
    |
    Published online on: June 19, 2013
       https://doi.org/10.3892/or.2013.2542
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Abstract

Dendropanax morbifera Leveille is found throughout southwestern Korea, and has been used in traditional medicine for various diseases, such as migraine headache, infectious diseases, skin diseases and dysmenorrhea. However, the molecular mechanisms of D. morbifera concerning its biochemical actions in cancer have not yet been clearly elucidated. In the present study, we investigated the pro-apoptotic effects of an ethanol extract of D. morbifera stem bark (EEDM) on human leukemia U937 cells. EEDM markedly inhibited the growth of U937 cells by decreasing cell proliferation and inducing apoptosis. EEDM-induced apoptosis in U937 cells was associated with the upregulation of death receptor-related protein levels and downregulation of anti-apoptotic IAP family proteins. The increase in apoptosis was also associated with proteolytic activation of caspase-8, -9 and -3, inhibition of anti‑apoptotic Bcl-2 and Bcl-xL expression, Bid cleavage, and loss of MMP suggesting that apoptosis of U937 cells induced by EEDM was through the extrinsic and intrinsic pathways. However, a pan-caspase inhibitor, z-VED-fmk, significantly inhibited EEDM-induced U937 cell apoptosis indicating that the caspases were key regulators of apoptosis in response to EEDM in U937 cells. Our data suggest that D. morbifera may be a potential anticancer agent for cancer treatment.
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1 

Jin Z and El-Deiry WS: Overview of cell death signaling pathways. Cancer Biol Ther. 4:139–163. 2005.PubMed/NCBI

2 

Fulda S and Debatin KM: Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene. 25:4798–4811. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Brenner D and Mak TW: Mitochondrial cell death effectors. Curr Opin Cell Biol. 21:871–877. 2009. View Article : Google Scholar

4 

Röder C, Trauzold A and Kalthoff H: Impact of death receptor signaling on the malignancy of pancreatic ductal adenocarcinoma. Eur J Cell Biol. 90:450–455. 2011.PubMed/NCBI

5 

Fulda S and Kroemer G: Mitochondria as therapeutic targets for the treatment of malignant disease. Antioxid Redox Signal. 15:2937–2949. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Kim IH, Kim SW, Kim SH, Lee SO, Lee ST, Kim DG, Lee MJ and Park WH: Parthenolide-induced apoptosis of hepatic stellate cells and anti-fibrotic effects in an in vivo rat model. Exp Mol Med. 44:448–456. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Ola MS, Nawaz M and Ahsan H: Role of Bcl-2 family proteins and caspases in the regulation of apoptosis. Mol Cell Biochem. 351:41–58. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Lazebnik YA, Kaufmann SH, Desnoyers S, Poirier GG and Earnshaw WC: Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature. 371:346–347. 1994. View Article : Google Scholar : PubMed/NCBI

9 

Kwon TK: Phorbol myristate acetate inhibits okadaic acid-induced apoptosis and downregulation of X-linked inhibitor of apoptosis in U937 cells. Biochem Biophys Res Commun. 287:135–141. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Fukuda K: Apoptosis-associated cleavage of β-catenin in human colon cancer and rat hepatoma cells. Int J Biochem Cell Biol. 31:519–529. 1999.

11 

Eskes R, Desagher S, Antonsson B and Martinou JC: Bid induces the oligomerization and insertion of Bax into the outer mitochondrial membrane. Mol Cell Biol. 20:929–935. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Shamas-Din A, Brahmbhatt H, Leber B and Andrews DW: BH3-only proteins: orchestrators of apoptosis. Biochim Biophys Acta. 1813:508–520. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Newman DJ and Cragg GM: Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod. 75:311–335. 2012.PubMed/NCBI

14 

Moon HI: Antidiabetic effects of dendropanoxide from leaves of Dendropanax morbifera Leveille in normal and streptozotocin-induced diabetic rats. Hum Exp Toxicol. 30:870–875. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Chung IM, Kim MY, Park WH and Moon HI: Antiatherogenic activity of Dendropanax morbifera essential oil in rats. Pharmazie. 64:547–549. 2009.

16 

Chung IM, Kim MY, Park SD, Park WH and Moon HI: In vitro evaluation of the antiplasmodial activity of Dendropanax morbifera against chloroquine-sensitive strains of Plasmodium falciparum. Phytother Res. 23:1634–1637. 2009.PubMed/NCBI

17 

Chung IM, Song HK, Kim SJ and Moon HI: Anticomplement activity of polyacetylenes from leaves of Dendropanax morbifera Leveille. Phytother Res. 25:784–786. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Park BY, Min BS, Oh SR, Kim JH, Kim TJ, Kim DH, Bae KH and Lee HK: Isolation and anticomplement activity of compounds from Dendropanax morbifera. J Ethnopharmacol. 90:403–408. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Richmond JD, Agius BR, Wright BS, Haber WA, Moriarity DM and Setzer WN: Essential oil compositions and cytotoxic activities of Dendropanax capillaris, Oreopanax nubigenus, and Schefflera rodrigueziana from Monteverde, Costa Rica. Nat Prod Commun. 4:271–274. 2009.PubMed/NCBI

20 

Yu HY, Kim KS, Lee YC, Moon HI and Lee JH: Oleifolioside A, a new active compound, attenuates LPS-stimulated iNOS and COX-2 expression through the downregulation of NF-κB and MAPK activities in RAW 264.7 macrophages. Evid Based Complement Alternat Med. 2012:6375122012.PubMed/NCBI

21 

Yu HY, Jin CY, Kim KS, Lee YC, Park SH, Kim GY, Kim WJ, Moon HI, Choi YH and Lee JH: Oleifolioside A mediates caspase-independent human cervical carcinoma HeLa cell apoptosis involving nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG. J Agric Food Chem. 60:5400–5406. 2012. View Article : Google Scholar

22 

Tak JK, Lee JH and Park JW: Resveratrol and piperine enhance radiosensitivity of tumor cells. BMB Rep. 45:242–246. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Nechushtan A, Smith CL, Hsu YT and Youle RJ: Conformation of the Bax C-terminus regulates subcellular location and cell death. EMBO J. 18:2330–2341. 1999. View Article : Google Scholar : PubMed/NCBI

24 

Danson S, Dean E, Dive C and Ranson M: IAPs as a target for anticancer therapy. Curr Cancer Drug Targets. 7:785–794. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Altieri DC: Survivin and IAP proteins in cell-death mechanisms. Biochem J. 430:199–205. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Xia Z, Dickens M, Raingeaud J, Davis RJ and Greenberg ME: Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science. 270:1326–1331. 1995. View Article : Google Scholar : PubMed/NCBI

27 

Ashkenazi A: Targeting the extrinsic apoptosis pathway in cancer. Cytokine Growth Factor Rev. 19:325–331. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Aksamitiene E, Kiyatkin A and Kholodenko BN: Cross-talk between mitogenic Ras/MAPK and survival PI3K/Akt pathways: a fine balance. Biochem Soc Trans. 40:139–146. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Boutros T, Chevet E and Metrakos P: Mitogen-activated protein (MAP) kinase/MAP kinase phosphatase regulation: roles in cell growth, death, and cancer. Pharmacol Rev. 60:261–310. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lee JW, Park C, Han MH, Hong SH, Lee TK, Lee SH, Kim G and Choi YH: Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway. Oncol Rep 30: 1231-1238, 2013.
APA
Lee, J.W., Park, C., Han, M.H., Hong, S.H., Lee, T.K., Lee, S.H. ... Choi, Y.H. (2013). Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway. Oncology Reports, 30, 1231-1238. https://doi.org/10.3892/or.2013.2542
MLA
Lee, J. W., Park, C., Han, M. H., Hong, S. H., Lee, T. K., Lee, S. H., Kim, G., Choi, Y. H."Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway". Oncology Reports 30.3 (2013): 1231-1238.
Chicago
Lee, J. W., Park, C., Han, M. H., Hong, S. H., Lee, T. K., Lee, S. H., Kim, G., Choi, Y. H."Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway". Oncology Reports 30, no. 3 (2013): 1231-1238. https://doi.org/10.3892/or.2013.2542
Copy and paste a formatted citation
x
Spandidos Publications style
Lee JW, Park C, Han MH, Hong SH, Lee TK, Lee SH, Kim G and Choi YH: Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway. Oncol Rep 30: 1231-1238, 2013.
APA
Lee, J.W., Park, C., Han, M.H., Hong, S.H., Lee, T.K., Lee, S.H. ... Choi, Y.H. (2013). Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway. Oncology Reports, 30, 1231-1238. https://doi.org/10.3892/or.2013.2542
MLA
Lee, J. W., Park, C., Han, M. H., Hong, S. H., Lee, T. K., Lee, S. H., Kim, G., Choi, Y. H."Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway". Oncology Reports 30.3 (2013): 1231-1238.
Chicago
Lee, J. W., Park, C., Han, M. H., Hong, S. H., Lee, T. K., Lee, S. H., Kim, G., Choi, Y. H."Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway". Oncology Reports 30, no. 3 (2013): 1231-1238. https://doi.org/10.3892/or.2013.2542
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