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Article

The insulin-like growth factor-1 and expression of its binding protein‑3 in chronic hepatitis C and hepatocellular carcinoma

  • Authors:
    • Agnieszka Adamek
    • Aldona Kasprzak
    • Hanna Mikoś
    • Wiesława  Przybyszewska
    • Agnieszka  Seraszek-Jaros
    • Arkadiusz  Czajka
    • Karolina  Sterzyńska
    • Iwona Mozer-Lisewska
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Diseases, Poznan University of Medical Sciences, Poznan, Poland, Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland, Department of Pediatric Endocrinology and Diabetes, Poznan University of Medical Sciences, Poznan, Poland, Department of Clinical Pathomorphology, Poznan University of Medical Sciences, Poznan, Poland
  • Pages: 1337-1345
    |
    Published online on: June 19, 2013
       https://doi.org/10.3892/or.2013.2546
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Abstract

The role of growth factors produced by the liver, including insulin-like growth factor-1 (IGF-1) and its main binding protein, IGF binding protein-3 (IGFBP-3), in hepatitis C virus (HCV)-associated carcinogenesis has only partially been recognized and there is not much data available on the local expression of IGF-1 and IGFBP-3 in chronic hepatitis C (CH‑C). Therefore, the aim of the present study was to evaluate the IGF‑1 and IGFBP‑3 serum levels and tissue expression in liver biopsies of CH‑C patients (n=37) and hepatocellular carcinoma (HCC) samples (n=61) as related to age- and gender-matched control serum samples (n=15) and healthy liver samples (n=10). Serum concentrations of IGF-1 (S-IGF-1) and IGFBP‑3 (S-IGFBP‑3) were measured by the ELISA method. Tissue expression of proteins was detected using ABC immunocytochemistry and evaluated applying a spatial visualization technique. Concentrations of S-IGF-1 and hepatic expression of IGF-1 (H-IGF-1) proved to be lower in CH-C compared to the controls. No significant differences were detected in the concentration of S-IGFBP-3 between the studied groups but the S-IGF-1/IGFBP-3 ratio in the CH-C group was significantly lower compared to the control. H-IGFBP-3 was higher in CH-C compared to those in the control and HCC. In HCC, lower expression of H-IGF-1 was detected compared to the control and a higher H-IGF-1/IGFBP-3 ratio compared to CH-C. A negative correlation was detected between S-IGF-1 and S-IGF-1/IGFBP-3 ratio, on the one hand, and age, grading and concentration of α-fetoprotein (AFP) on the other, while H-IGFBP-3 was negatively correlated with BMI in the CH‑C group. In patients with CH‑C, the H‑IGF‑1/IGFBP‑3 ratio was higher compared to that of the S‑IGF‑1/IGFBP‑3 ratio. The studies documented a disturbed H‑IGF‑1 and H‑IGFBP‑3 in CH‑C, which may be of significance in carcinogenesis. Examination of serum concentration and tissue expression of the two proteins and, first of all, estimation of the IGF‑1/IGFBP‑3 ratio may provide additional (to the estimation of IGF‑1 and AFP) non-invasive markers in HCV‑related liver injury.
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Copy and paste a formatted citation
Spandidos Publications style
Adamek A, Kasprzak A, Mikoś H, Przybyszewska W, Seraszek-Jaros A, Czajka A, Sterzyńska K and Mozer-Lisewska I: The insulin-like growth factor-1 and expression of its binding protein‑3 in chronic hepatitis C and hepatocellular carcinoma. Oncol Rep 30: 1337-1345, 2013.
APA
Adamek, A., Kasprzak, A., Mikoś, H., Przybyszewska, W., Seraszek-Jaros, A., Czajka, A. ... Mozer-Lisewska, I. (2013). The insulin-like growth factor-1 and expression of its binding protein‑3 in chronic hepatitis C and hepatocellular carcinoma. Oncology Reports, 30, 1337-1345. https://doi.org/10.3892/or.2013.2546
MLA
Adamek, A., Kasprzak, A., Mikoś, H., Przybyszewska, W., Seraszek-Jaros, A., Czajka, A., Sterzyńska, K., Mozer-Lisewska, I."The insulin-like growth factor-1 and expression of its binding protein‑3 in chronic hepatitis C and hepatocellular carcinoma". Oncology Reports 30.3 (2013): 1337-1345.
Chicago
Adamek, A., Kasprzak, A., Mikoś, H., Przybyszewska, W., Seraszek-Jaros, A., Czajka, A., Sterzyńska, K., Mozer-Lisewska, I."The insulin-like growth factor-1 and expression of its binding protein‑3 in chronic hepatitis C and hepatocellular carcinoma". Oncology Reports 30, no. 3 (2013): 1337-1345. https://doi.org/10.3892/or.2013.2546
Copy and paste a formatted citation
x
Spandidos Publications style
Adamek A, Kasprzak A, Mikoś H, Przybyszewska W, Seraszek-Jaros A, Czajka A, Sterzyńska K and Mozer-Lisewska I: The insulin-like growth factor-1 and expression of its binding protein‑3 in chronic hepatitis C and hepatocellular carcinoma. Oncol Rep 30: 1337-1345, 2013.
APA
Adamek, A., Kasprzak, A., Mikoś, H., Przybyszewska, W., Seraszek-Jaros, A., Czajka, A. ... Mozer-Lisewska, I. (2013). The insulin-like growth factor-1 and expression of its binding protein‑3 in chronic hepatitis C and hepatocellular carcinoma. Oncology Reports, 30, 1337-1345. https://doi.org/10.3892/or.2013.2546
MLA
Adamek, A., Kasprzak, A., Mikoś, H., Przybyszewska, W., Seraszek-Jaros, A., Czajka, A., Sterzyńska, K., Mozer-Lisewska, I."The insulin-like growth factor-1 and expression of its binding protein‑3 in chronic hepatitis C and hepatocellular carcinoma". Oncology Reports 30.3 (2013): 1337-1345.
Chicago
Adamek, A., Kasprzak, A., Mikoś, H., Przybyszewska, W., Seraszek-Jaros, A., Czajka, A., Sterzyńska, K., Mozer-Lisewska, I."The insulin-like growth factor-1 and expression of its binding protein‑3 in chronic hepatitis C and hepatocellular carcinoma". Oncology Reports 30, no. 3 (2013): 1337-1345. https://doi.org/10.3892/or.2013.2546
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