Advantages of the rapid double-staining method for intraoperative detection of micrometastasis in sentinel lymph nodes

Ojima,Toshihiko; Kinami,Shinichi; Nakamura,Keishi; Oyama,Katsunobu; Inokuchi,Masashi; Fujita,Hideto; Ninomiya,Itasu; Fushida,Sachio; Fujimura,Takashi; Kitamura,Seiko; Harada,Shinichi; Ohta,Tetsuo

doi:10.3892/or.2013.2591

September 2013 Volume 30 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

September 2013 Volume 30 Issue 3

Full Size Image

Article

Advantages of the rapid double-staining method for intraoperative detection of micrometastasis in sentinel lymph nodes

Authors:
- Toshihiko Ojima
- Shinichi Kinami
- Keishi Nakamura
- Katsunobu Oyama
- Masashi Inokuchi
- Hideto Fujita
- Itasu Ninomiya
- Sachio Fushida
- Takashi Fujimura
- Seiko Kitamura
- Shinichi Harada
- Tetsuo Ohta
View Affiliations / Copyright

Affiliations: Gastroenterologic Surgery, Department of Oncology, Division of Cancer Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8641, Japan, Department of Pathology, Division of Cancer Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8641, Japan, Center for Biomedical Research and Education, Division of Cancer Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8641, Japan
Pages: 1067-1072
|
Published online on: July 4, 2013

https://doi.org/10.3892/or.2013.2591
Expand metrics +

Abstract

For rapid intraoperative diagnosis of lymph node micrometastasis, we refined the rapid immunohistochemistry method by combining anti-cytokeratin antibody-labeled nanocrystal beads with rapid hematoxylin and eosin (H&E) staining on the same section, referred to as the rapid double staining (RDS) technique. Two frozen-section slices each were obtained from 372 lymph nodes of 100 breast cancer patients. We performed RDS for 1 slide and rapid H&E staining for the other. The results were compared with the corresponding final pathological data obtained from the permanent specimens. For specimens from patients with pN1(mi) as determined by final pathological examination, the false‑negative rate was 33.3% for rapid H&E staining and 16.7% for RDS. For specimens from patients with pN0(i+) as determined by final pathological examination, the false‑negative rate was 80% for rapid H&E staining and 0% for RDS. These results indicate that RDS is superior to conventional rapid H&E staining for intraoperative diagnosis of nodal micrometastasis and isolated tumor cells.

View Figures

View References

1	Ali R, Hanly AM, Naughton P, et al: Intraoperative frozen section assessment of sentinel lymph nodes in the operative management of women with symptomatic breast cancer. World J Surg Oncol. 6:692008. View Article : Google Scholar : PubMed/NCBI
2	Giobuin SM, Kavanagh DO, Myers E, et al: The significance of immunohistochemistry positivity in sentinel nodes which are negative on haematoxylin and eosin in breast cancer. Eur J Surg Oncol. 35:1257–1260. 2009. View Article : Google Scholar : PubMed/NCBI
3	Gobardhan PD, Elias SG, Madsen EV, et al: Prognostic value of micrometastases in sentinel lymph nodes of patients with breast carcinoma: a cohort study. Ann Oncol. 20:41–48. 2009. View Article : Google Scholar : PubMed/NCBI
4	de Boer M, van Deurzen CH, van Dijck JA, et al: Micrometastases or isolated tumor cells and the outcome of breast cancer. N Engl J Med. 361:653–663. 2009.
5	Mikhitarian K, Martin RH, Mitas M, et al: Molecular analysis improves sensitivity of breast sentinel lymph node biopsy: results of a multi-institutional prospective cohort study. Surgery. 138:474–481. 2005. View Article : Google Scholar
6	Fritzsche FR, Reineke T, Morawietz L, et al: Pathological processing techniques and final diagnosis of breast cancer sentinel lymph nodes. Ann Surg Oncol. 17:2892–2898. 2010. View Article : Google Scholar : PubMed/NCBI
7	Tsujimoto M, Nakabayashi K, Yoshidome K, et al: One-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients. Clin Cancer Res. 13:4807–4816. 2007. View Article : Google Scholar : PubMed/NCBI
8	Ishii K, Kinami S, Funaki K, et al: Detection of sentinel and non-sentinel lymph node micrometastases by complete serial sectioning and immunohistochemical analysis for gastric cancer. J Exp Clin Cancer Res. 27:72008. View Article : Google Scholar : PubMed/NCBI
9	Chattopadhyay PK, Price DA, Harper TF, et al: Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry. Nat Med. 12:972–977. 2006. View Article : Google Scholar : PubMed/NCBI
10	Chung I, Witkoskie JB, Cao J and Bawendi MG: Description of the fluorescence intensity time trace of collections of CdSe nanocrystal quantum dots based on single quantum dot fluorescence blinking statistics. Phys Rev E Stat Nonlin Soft Matter Phys. 73:0111062006. View Article : Google Scholar : PubMed/NCBI
11	Schmidt H, Brown EB, Schwaller B and Eilers J: Diffusional mobility of parvalbumin in spiny dendrites of cerebellar Purkinje neurons quantified by fluorescence recovery after photobleaching. Biophys J. 84:2599–2608. 2003. View Article : Google Scholar
12	Noguchi M, Inokuchi M and Zen Y: Complement of peritumoral and subareolar injection in breast cancer sentinel lymph node biopsy. J Surg Oncol. 100:100–105. 2009. View Article : Google Scholar : PubMed/NCBI
13	Turner RR, Ollila DW, Stern S and Giuliano AE: Optimal histopathologic examination of the sentinel lymph node for breast carcinoma staging. Am J Surg Pathol. 23:263–267. 1999. View Article : Google Scholar : PubMed/NCBI
14	Viale G, Dell'Orto P, Biasi MO, et al: Comparative evaluation of an extensive histopathologic examination and a real-time reverse-transcription-polymerase chain reaction assay for mammaglobin and cytokeratin 19 on axillary sentinel lymph nodes of breast carcinoma patients. Ann Surg. 247:136–142. 2008. View Article : Google Scholar
15	Tanis PJ, Boom RP, Koops HS, et al: Frozen section investigation of the sentinel node in malignant melanoma and breast cancer. Ann Surg Oncol. 8:222–226. 2001. View Article : Google Scholar : PubMed/NCBI
16	Hughes SJ, Xi L, Raja S, et al: A rapid, fully automated, molecular-based assay accurately analyzes sentinel lymph nodes for the presence of metastatic breast cancer. Ann Surg. 243:389–398. 2006. View Article : Google Scholar
17	Carmon M, Olsha O, Rivkin L, Spira RM and Golomb E: Intraoperative palpation for clinically suspicious axillary sentinel lymph nodes reduces the false-negative rate of sentinel lymph node biopsy in breast cancer. Breast J. 12:199–201. 2006. View Article : Google Scholar
18	Lifshitz E, Brumer M, Kigel A, et al: Air-stable PbSe/PbS and PbSe/PbSexS1-x core-shell nanocrystal quantum dots and their applications. J Phys Chem B. 110:25356–25365. 2006. View Article : Google Scholar : PubMed/NCBI
19	Charbonnière LJ, Hildebrandt N, Ziessel RF and Löhmannsröben HG: Lanthanides to quantum dots resonance energy transfer in time-resolved fluoro-immunoassays and luminescence microscopy. J Am Chem Soc. 128:12800–12809. 2006.PubMed/NCBI
20	Galimberti V: Axillary sentinel lymph node: how low can you go? Breast. 18(Suppl 1): S122009. View Article : Google Scholar
21	Nissan A, Jager D, Roystacher M, et al: Multimarker RT-PCR assay for the detection of minimal residual disease in sentinel lymph nodes of breast cancer patients. Br J Cancer. 94:681–685. 2006.PubMed/NCBI
22	Bostick PJ, Huynh KT, Sarantou T, et al: Detection of metastases in sentinel lymph nodes of breast cancer patients by multiple-marker RT-PCR. Int J Cancer. 79:645–651. 1998. View Article : Google Scholar : PubMed/NCBI
23	Schem C, Maass N, Bauerschlag DO, et al: One-step nucleic acid amplification - a molecular method for the detection of lymph node metastases in breast cancer patients; results of the German study group. Virchows Arch. 454:203–210. 2009. View Article : Google Scholar : PubMed/NCBI
24	Tamaki Y, Akiyama F, Iwase T, et al: Molecular detection of lymph node metastases in breast cancer patients: results of a multicenter trial using the one-step nucleic acid amplification assay. Clin Cancer Res. 15:2879–2884. 2009. View Article : Google Scholar : PubMed/NCBI
25	Parikh RR, Yang Q, Higgins SA and Haffty BG: Outcomes in young women with breast cancer of triple-negative phenotype: the prognostic significance of CK19 expression. Int J Radiat Oncol Biol Phys. 70:35–42. 2008. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Advantages of the rapid double-staining method for intraoperative detection of micrometastasis in sentinel lymph nodes

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Related Articles