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Article

CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer

  • Authors:
    • Genta Sawada
    • Hiroki Ueo
    • Tae Matsumura
    • Ryutaro Uchi
    • Masahisa Ishibashi
    • Kosuke Mima
    • Junji Kurashige
    • Yusuke Takahashi
    • Sayori Akiyoshi
    • Tomoya Sudo
    • Keishi Sugimachi
    • Yuichiro Doki
    • Masaki Mori
    • Koshi Mimori
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Beppu Hospital, Kyushu University, Beppu 874-0838, Japan, Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita 565-0871, Japan
  • Pages: 1137-1142
    |
    Published online on: July 8, 2013
       https://doi.org/10.3892/or.2013.2597
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Abstract

The chromodomain helicase DNA-binding (CHD) family comprises a class of chromatin remodeling enzymes. Previous studies suggest that CHD8 may negatively regulate various genes and signaling pathways, such as the Wnt/β‑catenin pathway. However, few studies have investigated the role of CHD8 in cancer cells. We analyzed the expression of CHD8 in cancer lesions and corresponding non-cancerous tissues to demonstrate the prognostic significance of CHD8 expression in 101 cases of gastric cancer. We also investigated the functional implications of aberrant CHD8 expression by conducting gene set enrichment analysis (GSEA). Expression of CHD8 mRNA was significantly lower in gastric cancer tissues compared to that in corresponding normal tissues (P=0.003). In multivariate analysis for overall survival, we found that CHD8 expression was an independent prognostic factor in gastric cancer. Moreover, GSEA revealed that CHD8 was significantly associated with genes involved in the Wnt/β‑catenin pathway and in the cell cycle. In addition, knockdown of CHD8 expression in the gastric cancer cell lines, MKN45 and NUGC4, promoted proliferation. In conclusion, the present study suggests that loss of CHD8 expression may be a novel indicator for biological aggressiveness in gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Sawada G, Ueo H, Matsumura T, Uchi R, Ishibashi M, Mima K, Kurashige J, Takahashi Y, Akiyoshi S, Sudo T, Sudo T, et al: CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer. Oncol Rep 30: 1137-1142, 2013.
APA
Sawada, G., Ueo, H., Matsumura, T., Uchi, R., Ishibashi, M., Mima, K. ... Mimori, K. (2013). CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer. Oncology Reports, 30, 1137-1142. https://doi.org/10.3892/or.2013.2597
MLA
Sawada, G., Ueo, H., Matsumura, T., Uchi, R., Ishibashi, M., Mima, K., Kurashige, J., Takahashi, Y., Akiyoshi, S., Sudo, T., Sugimachi, K., Doki, Y., Mori, M., Mimori, K."CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer". Oncology Reports 30.3 (2013): 1137-1142.
Chicago
Sawada, G., Ueo, H., Matsumura, T., Uchi, R., Ishibashi, M., Mima, K., Kurashige, J., Takahashi, Y., Akiyoshi, S., Sudo, T., Sugimachi, K., Doki, Y., Mori, M., Mimori, K."CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer". Oncology Reports 30, no. 3 (2013): 1137-1142. https://doi.org/10.3892/or.2013.2597
Copy and paste a formatted citation
x
Spandidos Publications style
Sawada G, Ueo H, Matsumura T, Uchi R, Ishibashi M, Mima K, Kurashige J, Takahashi Y, Akiyoshi S, Sudo T, Sudo T, et al: CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer. Oncol Rep 30: 1137-1142, 2013.
APA
Sawada, G., Ueo, H., Matsumura, T., Uchi, R., Ishibashi, M., Mima, K. ... Mimori, K. (2013). CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer. Oncology Reports, 30, 1137-1142. https://doi.org/10.3892/or.2013.2597
MLA
Sawada, G., Ueo, H., Matsumura, T., Uchi, R., Ishibashi, M., Mima, K., Kurashige, J., Takahashi, Y., Akiyoshi, S., Sudo, T., Sugimachi, K., Doki, Y., Mori, M., Mimori, K."CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer". Oncology Reports 30.3 (2013): 1137-1142.
Chicago
Sawada, G., Ueo, H., Matsumura, T., Uchi, R., Ishibashi, M., Mima, K., Kurashige, J., Takahashi, Y., Akiyoshi, S., Sudo, T., Sugimachi, K., Doki, Y., Mori, M., Mimori, K."CHD8 is an independent prognostic indicator that regulates Wnt/β-catenin signaling and the cell cycle in gastric cancer". Oncology Reports 30, no. 3 (2013): 1137-1142. https://doi.org/10.3892/or.2013.2597
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