Overexpression of adenylate cyclase-associated protein 1 is associated with metastasis of lung cancer

Tan,Min; Song,Xiaolian; Zhang,Guoliang; Peng,Aimei; Li,Xuan; Li,Ming; Liu,Yang; Wang,Changhui

doi:10.3892/or.2013.2607

October 2013 Volume 30 Issue 4

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October 2013 Volume 30 Issue 4

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Article

Overexpression of adenylate cyclase-associated protein 1 is associated with metastasis of lung cancer

Authors:
- Min Tan
- Xiaolian Song
- Guoliang Zhang
- Aimei Peng
- Xuan Li
- Ming Li
- Yang Liu
- Changhui Wang
View Affiliations / Copyright

Affiliations: Department of Respiratory Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, P.R. China
Pages: 1639-1644
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Published online on: July 9, 2013

https://doi.org/10.3892/or.2013.2607
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Abstract

Lung cancer ranks first in both prevalence and mortality rates among all types of cancer. Metastasis is the main cause of treatment failure. Biomarkers are critical to early diagnosis and prediction and monitoring of progressive lesions. Several biomarkers have been identified for lung cancer but none have been routinely used clinically. The present study assessed the diagnostic and prognostic value of cyclase-associated protein 1 (CAP1) for lung cancer. CAP1 mRNA abundance and protein content were determined by real-time PCR and western blot analysis and/or immunostaining in biopsy specimens (24 neoplastic and 6 non-neoplastic) freshly collected at surgical lung resection, in 82 pathologically banked lung cancer specimens and in cultured non-invasive (95-C) and invasive (95-D) lung cancer cells. Multivariate regression analysis was performed to correlate immunoreactive CAP1 signal with cancer type and stage. In vitro cell migration was performed to determine the effect of RNA interference-mediated CAP1 gene silencing on invasiveness of 95-D cells. These analyses collectively demonstrated that: i) both CAP1 mRNA abundance and protein content were significantly higher in neoplastic compared to non-neoplastic specimens and in metastatic compared to non-metastatic specimens but not different between adenocarcinoma and squamous cell carcinoma; ii) immunoreactive CAP1 signal was significantly stronger in metastatic specimens and 95-D cells compared to non-metastatic specimens and 95-C cells; and iii) RNA interference-mediated CAP1 gene silencing adequately attenuated the invasive capacity of 95-D cells in vitro. These findings suggest that overexpression of CAP1 in lung cancer cells, particularly at the metastatic stage, may have significant clinical implications as a diagnostic/prognostic factor for lung cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Overexpression of adenylate cyclase-associated protein 1 is associated with metastasis of lung cancer

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