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Inhibition of the transcription factor Sp1 suppresses colon cancer stem cell growth and induces apoptosis in vitro and in nude mouse xenografts

  • Authors:
    • Yingying Zhao
    • Wenjing Zhang
    • Zheng Guo
    • Feng Ma
    • Yao Wu
    • Yang Bai
    • Wei Gong
    • Ye Chen
    • Tianming Cheng
    • Fachao Zhi
    • Yali Zhang
    • Jide Wang
    • Bo Jiang

  • View Affiliations

  • Published online on: July 18, 2013     https://doi.org/10.3892/or.2013.2627
  • Pages: 1782-1792
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Abstract

The transcription factor specificity protein 1 (Sp1) plays a role in the development and progression of various types of human cancers, while cancer stem cells (CSCs) are important in cancer cell self-renewal, resistance to chemotherapy and metastatic potential. This study investigated the role of Sp1 in colon CSC growth and apoptosis. Colon CSCs were successfully enriched using special culture medium and identified by typical CSC gene expression. In a quiescent state, these CSCs formed spheres with slow proliferation; overexpressed Sp1, CD44, CD166 and CD133 proteins; upregulated mesenchymal markers; and a downregulated epithelial marker were noted. In ex vivo experiments, the Sp1 protein was expressed in 74.8% of colon cancer tissues, whereas it was expressed only in 42.2% of the distant normal colon mucosae. Furthermore, inhibition of SP1 expression using Sp1 siRNA or mithramycin A (MIT) led to marked suppression of CSC growth and induced apoptosis. In addition, the percentage of CD44+/CD166+ cells was significantly downregulated both in vivo and in vitro following Sp1 inhibition. In conclusion, Sp1 suppression attenuated the characteristics of colon CSCs. Thus, Sp1 inhibition may be potentially useful for the future development of a novel therapeutic strategy to control colon cancer.
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Journal Cover

October 2013
Volume 30 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Journal Metrics
  • Impact Factor: 4.2
  • Ranked Oncology
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  • CiteScore: 8.4
  • CiteScore Rank: Q1: #68/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.786
  • SCImago Journal Rank (SJR): 0.812
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Spandidos Publications style
Zhao Y, Zhang W, Guo Z, Ma F, Wu Y, Bai Y, Gong W, Chen Y, Cheng T, Zhi F, Zhi F, et al: Inhibition of the transcription factor Sp1 suppresses colon cancer stem cell growth and induces apoptosis in vitro and in nude mouse xenografts . Oncol Rep 30: 1782-1792, 2013
APA
Zhao, Y., Zhang, W., Guo, Z., Ma, F., Wu, Y., Bai, Y. ... Jiang, B. (2013). Inhibition of the transcription factor Sp1 suppresses colon cancer stem cell growth and induces apoptosis in vitro and in nude mouse xenografts . Oncology Reports, 30, 1782-1792. https://doi.org/10.3892/or.2013.2627
MLA
Zhao, Y., Zhang, W., Guo, Z., Ma, F., Wu, Y., Bai, Y., Gong, W., Chen, Y., Cheng, T., Zhi, F., Zhang, Y., Wang, J., Jiang, B."Inhibition of the transcription factor Sp1 suppresses colon cancer stem cell growth and induces apoptosis in vitro and in nude mouse xenografts ". Oncology Reports 30.4 (2013): 1782-1792.
Chicago
Zhao, Y., Zhang, W., Guo, Z., Ma, F., Wu, Y., Bai, Y., Gong, W., Chen, Y., Cheng, T., Zhi, F., Zhang, Y., Wang, J., Jiang, B."Inhibition of the transcription factor Sp1 suppresses colon cancer stem cell growth and induces apoptosis in vitro and in nude mouse xenografts ". Oncology Reports 30, no. 4 (2013): 1782-1792. https://doi.org/10.3892/or.2013.2627