Apoptosis induced by PGC-1β in breast cancer cells is mediated by the mTOR pathway

  • Authors:
    • Libin Wang
    • Qilun Liu
    • Fang Li
    • Jing Qiu
    • Heng Fan
    • Haibin Ma
    • Yongzhao Zhu
    • Ligang Wu
    • Xuebo Han
    • Zhihong Yang
    • Haifeng Jiang
    • Jun Wei
    • Haibin Xia
  • View Affiliations

  • Published online on: July 18, 2013     https://doi.org/10.3892/or.2013.2628
  • Pages: 1631-1638
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Abstract

The peroxisome proliferator-activated receptor-γ (PPAR-γ) coactivator-1β (PGC-1β) is a well-established regulator of mitochondrial biogenesis. However, the underlying mechanism of PGC-1β action remains elusive. This study reveals that knockdown of endogenous PGC-1β by short-hairpin RNA (shRNA) leads to a decrease in the expression of mammalian target of rapamycin (mTOR) pathway-related genes in MDA-MB-231 cells. After knockdown of PGC-1β, phosphorylation of AMP-activated protein kinase (AMPK), phosphorylation of Rictor on Thr1135, Raptor and S6 protein was inhibited. However, Akt phosphorylation on Ser473 was upregulated and cell apoptosis occurred. In particular, we demonstrate that the levels of PGC-1β and mTOR correlated with overall mitochondrial activity. These results provide new evidence that cell apoptosis is orchestrated by the balance between several signaling pathways, and that PGC-1β takes part in these events in breast cancer cells mediated by the mTOR signaling pathway.
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October 2013
Volume 30 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang L, Liu Q, Li F, Qiu J, Fan H, Ma H, Zhu Y, Wu L, Han X, Yang Z, Yang Z, et al: Apoptosis induced by PGC-1β in breast cancer cells is mediated by the mTOR pathway. Oncol Rep 30: 1631-1638, 2013.
APA
Wang, L., Liu, Q., Li, F., Qiu, J., Fan, H., Ma, H. ... Xia, H. (2013). Apoptosis induced by PGC-1β in breast cancer cells is mediated by the mTOR pathway. Oncology Reports, 30, 1631-1638. https://doi.org/10.3892/or.2013.2628
MLA
Wang, L., Liu, Q., Li, F., Qiu, J., Fan, H., Ma, H., Zhu, Y., Wu, L., Han, X., Yang, Z., Jiang, H., Wei, J., Xia, H."Apoptosis induced by PGC-1β in breast cancer cells is mediated by the mTOR pathway". Oncology Reports 30.4 (2013): 1631-1638.
Chicago
Wang, L., Liu, Q., Li, F., Qiu, J., Fan, H., Ma, H., Zhu, Y., Wu, L., Han, X., Yang, Z., Jiang, H., Wei, J., Xia, H."Apoptosis induced by PGC-1β in breast cancer cells is mediated by the mTOR pathway". Oncology Reports 30, no. 4 (2013): 1631-1638. https://doi.org/10.3892/or.2013.2628