microRNA-155 regulates cell proliferation and invasion by targeting FOXO3a in glioma

  • Authors:
    • Nan Ling
    • Junyi Gu
    • Zhe Lei
    • Mengmeng Li
    • Jun Zhao
    • Hong-Tao Zhang
    • Xiangdong Li
  • View Affiliations

  • Published online on: August 22, 2013     https://doi.org/10.3892/or.2013.2685
  • Pages: 2111-2118
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Abstract

microRNAs (miRNAs) are short noncoding RNAs, which modulate the expression of numerous genes by targeting mRNAs. Numerous abnormal miRNA expression patterns are found in various human malignancies, and certain miRNAs act as oncogenes or tumor suppressors. microRNA-155 (miR‑155) may not only function as an oncogene but also as a tumor suppressor in various types of cancer cells, such as melanoma. Although miR-155 has been found to be upregulated in glioma, its role has not yet been eludicated in glioma tumorigenesis. Based on the prediction of the target genes of miR-155, we hypothesized that there is a significant association between miR-155 and FOXO3a, a negative regulator of Akt signaling. In the present study, we found that FOXO3a expression was significantly downregulated and miR-155 was upregulated in a panel of glioma cells and tissue specimens. Furthermore, we demonstrated that miR-155 induced cell proliferation by inhibiting apoptosis and promoted the migration and invasiveness of glioma cells, while miR-155 had no effect on the cell cycle as determined by gain-of-function and loss-of-function experiments. Moreover, we confirmed that miR-155 downregulated the expression of FOXO3a by directly targeting its 3'-UTR. These findings indicate that miR-155 may function as an oncogene by targeting FOXO3a in the development and progression of glioma.
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November 2013
Volume 30 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ling N, Gu J, Lei Z, Li M, Zhao J, Zhang H and Li X: microRNA-155 regulates cell proliferation and invasion by targeting FOXO3a in glioma. Oncol Rep 30: 2111-2118, 2013
APA
Ling, N., Gu, J., Lei, Z., Li, M., Zhao, J., Zhang, H., & Li, X. (2013). microRNA-155 regulates cell proliferation and invasion by targeting FOXO3a in glioma. Oncology Reports, 30, 2111-2118. https://doi.org/10.3892/or.2013.2685
MLA
Ling, N., Gu, J., Lei, Z., Li, M., Zhao, J., Zhang, H., Li, X."microRNA-155 regulates cell proliferation and invasion by targeting FOXO3a in glioma". Oncology Reports 30.5 (2013): 2111-2118.
Chicago
Ling, N., Gu, J., Lei, Z., Li, M., Zhao, J., Zhang, H., Li, X."microRNA-155 regulates cell proliferation and invasion by targeting FOXO3a in glioma". Oncology Reports 30, no. 5 (2013): 2111-2118. https://doi.org/10.3892/or.2013.2685