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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

B7-H1 protein vaccine induces protective and therapeutic antitumor responses in SP2/0 myeloma-bearing mice

  • Authors:
    • Cun Zhang
    • Weihua Wang
    • Xin Qin
    • Yujin Xu
    • Tonglie Huang
    • Qiang Hao
    • Weina Li
    • Shouzhen Wu
    • Yingqi Zhang
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 2442-2448
    |
    Published online on: August 22, 2013
       https://doi.org/10.3892/or.2013.2686
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Abstract

B7-H1 is a co-inhibitory molecule belonging to the B7 family. The B7-H1 protein is only expressed on macrophage lineage of cells in normal tissues, but is overexpressed in most types of tumor. The aberrant expression of cell surface B7-H1 on cancer cells is generally associated with high-risk prognostic factors. The tumor-associated B7-H1 increases apoptosis of antigen-specific T cells through interaction with its receptor PD-1 on CD8+ T cells and contributes to tumor immune evasion. These features suggest that B7-H1 may be a therapeutic target for the B7-H1-expressing tumors. We developed a therapeutic vaccine by coupling a tetanus toxoid T-helper cell epitope with the N-terminal of B7-H1 IgV-like domain. This vaccine was able to induce high titers of antibodies against B7-H1 in mice which were able to bind to native cell surface B7-H1. We chose the B7-H1-expressing SP2/0 myeloma and its syngeneic host (the BALB/c mouse) as the model to study the antitumor activity of the rhB7-H1M vaccine. Vaccination with this modified B7-H1 protein resulted in almost complete protection from SP2/0 tumor challenge and efficiently eliminated pre-established tumors in mice. In addition, B7-H1 vaccination was able to decrease the percentage of CD4+ Foxp3+ regulatory T cells in tumor-bearing mice and which might improve antitumor immunity. These data demonstrate the potential of B7-H1-based vaccine as a therapeutic agent for the treatment of cancer overexpressing B7-H1.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang C, Wang W, Qin X, Xu Y, Huang T, Hao Q, Li W, Wu S and Zhang Y: B7-H1 protein vaccine induces protective and therapeutic antitumor responses in SP2/0 myeloma-bearing mice. Oncol Rep 30: 2442-2448, 2013.
APA
Zhang, C., Wang, W., Qin, X., Xu, Y., Huang, T., Hao, Q. ... Zhang, Y. (2013). B7-H1 protein vaccine induces protective and therapeutic antitumor responses in SP2/0 myeloma-bearing mice. Oncology Reports, 30, 2442-2448. https://doi.org/10.3892/or.2013.2686
MLA
Zhang, C., Wang, W., Qin, X., Xu, Y., Huang, T., Hao, Q., Li, W., Wu, S., Zhang, Y."B7-H1 protein vaccine induces protective and therapeutic antitumor responses in SP2/0 myeloma-bearing mice". Oncology Reports 30.5 (2013): 2442-2448.
Chicago
Zhang, C., Wang, W., Qin, X., Xu, Y., Huang, T., Hao, Q., Li, W., Wu, S., Zhang, Y."B7-H1 protein vaccine induces protective and therapeutic antitumor responses in SP2/0 myeloma-bearing mice". Oncology Reports 30, no. 5 (2013): 2442-2448. https://doi.org/10.3892/or.2013.2686
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang C, Wang W, Qin X, Xu Y, Huang T, Hao Q, Li W, Wu S and Zhang Y: B7-H1 protein vaccine induces protective and therapeutic antitumor responses in SP2/0 myeloma-bearing mice. Oncol Rep 30: 2442-2448, 2013.
APA
Zhang, C., Wang, W., Qin, X., Xu, Y., Huang, T., Hao, Q. ... Zhang, Y. (2013). B7-H1 protein vaccine induces protective and therapeutic antitumor responses in SP2/0 myeloma-bearing mice. Oncology Reports, 30, 2442-2448. https://doi.org/10.3892/or.2013.2686
MLA
Zhang, C., Wang, W., Qin, X., Xu, Y., Huang, T., Hao, Q., Li, W., Wu, S., Zhang, Y."B7-H1 protein vaccine induces protective and therapeutic antitumor responses in SP2/0 myeloma-bearing mice". Oncology Reports 30.5 (2013): 2442-2448.
Chicago
Zhang, C., Wang, W., Qin, X., Xu, Y., Huang, T., Hao, Q., Li, W., Wu, S., Zhang, Y."B7-H1 protein vaccine induces protective and therapeutic antitumor responses in SP2/0 myeloma-bearing mice". Oncology Reports 30, no. 5 (2013): 2442-2448. https://doi.org/10.3892/or.2013.2686
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