BTG1 inhibits breast cancer cell growth through induction of cell cycle arrest and apoptosis

  • Authors:
    • Ran Zhu
    • Shi-Tao Zou
    • Jian-Mei Wan
    • Wei Li
    • Xin-Li Li
    • Wei Zhu
  • View Affiliations

  • Published online on: August 26, 2013     https://doi.org/10.3892/or.2013.2697
  • Pages: 2137-2144
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Abstract

BTG1, which belongs to the BTG/Tob family, regulates cell cycle progression in a variety of cell types and appears to play roles in inhibiting proliferation, promoting apoptosis and stimulating cellular differentiation in multiple cell types. However, it remains unclear whether BTG1 is a breast cancer suppressor gene, and the role of BTG1 in breast cancer cell growth has not yet been determined. In the present study, we observed that BTG1 was weakly expressed in human breast tumors and in breast cancer cells (MCF-7 and MDA-MB-231). In addition, we investigated the potential effects of BTG1 on breast cancer cell proliferation, cell cycle distribution and apoptosis after stable transfection with the BTG1 expression vector. We found that overexpression of BTG1 inhibited cell proliferation, induced G0/G1 cell cycle arrest and promoted apoptosis. Further investigation indicated that overexpression of BTG1 was involved in the inhibition of the expression of cell cycle-related proteins, cyclin B1 and cyclin D1, and pro-apoptotic factors, Bax and caspase-3, and was also involved in the promotion of anti-apoptotic factor Bcl-2. In vivo, animal experiments showed that tumors overexpressing BTG1 displayed a slower growth rate than the control xenografts. TUNEL end staining assay revealed that BTG1 induced tumor necrosis and apoptosis. Taken together, our data revealed that, in breast cancer cells, BTG1 inhibits cell growth through induction of cell cycle arrest and apoptosis. These results indicate that BTG1 may be used as a novel therapeutic target for human breast cancer treatment.
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November 2013
Volume 30 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhu R, Zou S, Wan J, Li W, Li X and Zhu W: BTG1 inhibits breast cancer cell growth through induction of cell cycle arrest and apoptosis. Oncol Rep 30: 2137-2144, 2013
APA
Zhu, R., Zou, S., Wan, J., Li, W., Li, X., & Zhu, W. (2013). BTG1 inhibits breast cancer cell growth through induction of cell cycle arrest and apoptosis. Oncology Reports, 30, 2137-2144. https://doi.org/10.3892/or.2013.2697
MLA
Zhu, R., Zou, S., Wan, J., Li, W., Li, X., Zhu, W."BTG1 inhibits breast cancer cell growth through induction of cell cycle arrest and apoptosis". Oncology Reports 30.5 (2013): 2137-2144.
Chicago
Zhu, R., Zou, S., Wan, J., Li, W., Li, X., Zhu, W."BTG1 inhibits breast cancer cell growth through induction of cell cycle arrest and apoptosis". Oncology Reports 30, no. 5 (2013): 2137-2144. https://doi.org/10.3892/or.2013.2697