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Article

Ibuprofen enhances TRAIL-induced apoptosis through DR5 upregulation

  • Authors:
    • Momoko Todo
    • Mano Horinaka
    • Mitsuhiro Tomosugi
    • Ryoichi Tanaka
    • Haruna Ikawa
    • Yoshihiro Sowa
    • Hideki Ishikawa
    • Hitoshi Fujiwara
    • Eigo Otsuji
    • Toshiyuki Sakai
  • View Affiliations / Copyright

    Affiliations: Department of Molecular-Targeting Cancer Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan, Department of Digestive Surgery, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan
  • Pages: 2379-2384
    |
    Published online on: August 30, 2013
       https://doi.org/10.3892/or.2013.2713
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Abstract

Numerous human chemoprevention studies have demonstrated that non-steroidal anti-inflammatory drugs (NSAIDs) possess chemopreventive effects against a variety of malignant tumors. However, there have been many clinical studies on aspirin, but not ibuprofen, even though ibuprofen is one of the most clinically and safely used NSAIDs showing potent anti-inflammatory effects. Moreover, we reported that many chemopreventive agents enhance the apoptosis-inducing effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is known to be crucial for cancer prevention. We, therefore, investigated whether ibuprofen enhances the cytocidal effect of TRAIL and found that ibuprofen markedly stimulated the apoptosis-inducing efficacy of TRAIL against human colon cancer HCT116 cells. As detected by western blot analysis and real-time RT-PCR, ibuprofen upregulated the expression of death receptor 5 (DR5), a TRAIL receptor. TRAIL-induced apoptosis enhanced by ibuprofen was effectively decreased by a caspase inhibitor and dominant-negative DR5. Noteworthy, co-treatment of ibuprofen with TRAIL did not enhance apoptosis in normal peripheral blood mononuclear cells (PBMCs). These results demonstrated that ibuprofen and TRAIL synergistically induced apoptosis in human colon cancer HCT116 cells but not in normal PBMCs, raising the possibility that ibuprofen may be promising as a safe chemopreventive agent against colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Todo M, Horinaka M, Tomosugi M, Tanaka R, Ikawa H, Sowa Y, Ishikawa H, Fujiwara H, Otsuji E, Sakai T, Sakai T, et al: Ibuprofen enhances TRAIL-induced apoptosis through DR5 upregulation. Oncol Rep 30: 2379-2384, 2013.
APA
Todo, M., Horinaka, M., Tomosugi, M., Tanaka, R., Ikawa, H., Sowa, Y. ... Sakai, T. (2013). Ibuprofen enhances TRAIL-induced apoptosis through DR5 upregulation. Oncology Reports, 30, 2379-2384. https://doi.org/10.3892/or.2013.2713
MLA
Todo, M., Horinaka, M., Tomosugi, M., Tanaka, R., Ikawa, H., Sowa, Y., Ishikawa, H., Fujiwara, H., Otsuji, E., Sakai, T."Ibuprofen enhances TRAIL-induced apoptosis through DR5 upregulation". Oncology Reports 30.5 (2013): 2379-2384.
Chicago
Todo, M., Horinaka, M., Tomosugi, M., Tanaka, R., Ikawa, H., Sowa, Y., Ishikawa, H., Fujiwara, H., Otsuji, E., Sakai, T."Ibuprofen enhances TRAIL-induced apoptosis through DR5 upregulation". Oncology Reports 30, no. 5 (2013): 2379-2384. https://doi.org/10.3892/or.2013.2713
Copy and paste a formatted citation
x
Spandidos Publications style
Todo M, Horinaka M, Tomosugi M, Tanaka R, Ikawa H, Sowa Y, Ishikawa H, Fujiwara H, Otsuji E, Sakai T, Sakai T, et al: Ibuprofen enhances TRAIL-induced apoptosis through DR5 upregulation. Oncol Rep 30: 2379-2384, 2013.
APA
Todo, M., Horinaka, M., Tomosugi, M., Tanaka, R., Ikawa, H., Sowa, Y. ... Sakai, T. (2013). Ibuprofen enhances TRAIL-induced apoptosis through DR5 upregulation. Oncology Reports, 30, 2379-2384. https://doi.org/10.3892/or.2013.2713
MLA
Todo, M., Horinaka, M., Tomosugi, M., Tanaka, R., Ikawa, H., Sowa, Y., Ishikawa, H., Fujiwara, H., Otsuji, E., Sakai, T."Ibuprofen enhances TRAIL-induced apoptosis through DR5 upregulation". Oncology Reports 30.5 (2013): 2379-2384.
Chicago
Todo, M., Horinaka, M., Tomosugi, M., Tanaka, R., Ikawa, H., Sowa, Y., Ishikawa, H., Fujiwara, H., Otsuji, E., Sakai, T."Ibuprofen enhances TRAIL-induced apoptosis through DR5 upregulation". Oncology Reports 30, no. 5 (2013): 2379-2384. https://doi.org/10.3892/or.2013.2713
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