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Article

Pretreatment of baicalin and wogonoside with glycoside hydrolase: A promising approach to enhance anticancer potential

  • Authors:
    • Chunhao Yu
    • Zhiyu Zhang
    • Haijiang Zhang
    • Zhong Zhen
    • Tyler Calway
    • Yunwei Wang
    • Chun-Su Yuan
    • Chong-Zhi Wang
  • View Affiliations / Copyright

    Affiliations: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Jiangsu 223003, P.R. China, Tang Center for Herbal Medicine Research, University of Chicago, Chicago, IL 60637, USA, Department of Medicine, University of Chicago, Chicago, IL 60637, USA
  • Pages: 2411-2418
    |
    Published online on: September 9, 2013
       https://doi.org/10.3892/or.2013.2726
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Abstract

Previous phytochemical studies showed that the major flavonoids in Scutellaria baicalensis are baicalin, baicalein, wogonoside and wogonin. The two glycosides (baicalin and wogonoside) can be transformed into their aglycons (baicalein and wogonin), which possess positive anticancer potential. In this study, we used glycosidase to catalyze flavonoids in S. baicalensis to enhance the herb's anticancer activities. Our HPLC data showed that, using the optimized conditions obtained in our experiments (20 U/g of cellulase, 50˚C, pH 4.8 and treatment for 8 h), there was a marked transformation from the two glycosides to their aglycons. The anticancer activity was subsequently evaluated using a series of S. baicalensis extracts in which variable lengths of glycosidase treatment time were used. Combining analytical and bioassay results, we observed that the higher the aglycon content, the stronger the antiproliferation effects. Compared to the untransformed control, 8 h of glycosidase catalyzing significantly increased antiproliferative activity on human colorectal and breast cancer cells, and its cancer cell growth inhibition is, in part, mediated by cell cycle arrest at the S-phase and induction of apoptosis. Data from this study suggest that using glycosidase to catalyze S. baicalensis offers a promising approach to increase its anticancer activity.
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Copy and paste a formatted citation
Spandidos Publications style
Yu C, Zhang Z, Zhang H, Zhen Z, Calway T, Wang Y, Yuan C and Wang C: Pretreatment of baicalin and wogonoside with glycoside hydrolase: A promising approach to enhance anticancer potential. Oncol Rep 30: 2411-2418, 2013.
APA
Yu, C., Zhang, Z., Zhang, H., Zhen, Z., Calway, T., Wang, Y. ... Wang, C. (2013). Pretreatment of baicalin and wogonoside with glycoside hydrolase: A promising approach to enhance anticancer potential. Oncology Reports, 30, 2411-2418. https://doi.org/10.3892/or.2013.2726
MLA
Yu, C., Zhang, Z., Zhang, H., Zhen, Z., Calway, T., Wang, Y., Yuan, C., Wang, C."Pretreatment of baicalin and wogonoside with glycoside hydrolase: A promising approach to enhance anticancer potential". Oncology Reports 30.5 (2013): 2411-2418.
Chicago
Yu, C., Zhang, Z., Zhang, H., Zhen, Z., Calway, T., Wang, Y., Yuan, C., Wang, C."Pretreatment of baicalin and wogonoside with glycoside hydrolase: A promising approach to enhance anticancer potential". Oncology Reports 30, no. 5 (2013): 2411-2418. https://doi.org/10.3892/or.2013.2726
Copy and paste a formatted citation
x
Spandidos Publications style
Yu C, Zhang Z, Zhang H, Zhen Z, Calway T, Wang Y, Yuan C and Wang C: Pretreatment of baicalin and wogonoside with glycoside hydrolase: A promising approach to enhance anticancer potential. Oncol Rep 30: 2411-2418, 2013.
APA
Yu, C., Zhang, Z., Zhang, H., Zhen, Z., Calway, T., Wang, Y. ... Wang, C. (2013). Pretreatment of baicalin and wogonoside with glycoside hydrolase: A promising approach to enhance anticancer potential. Oncology Reports, 30, 2411-2418. https://doi.org/10.3892/or.2013.2726
MLA
Yu, C., Zhang, Z., Zhang, H., Zhen, Z., Calway, T., Wang, Y., Yuan, C., Wang, C."Pretreatment of baicalin and wogonoside with glycoside hydrolase: A promising approach to enhance anticancer potential". Oncology Reports 30.5 (2013): 2411-2418.
Chicago
Yu, C., Zhang, Z., Zhang, H., Zhen, Z., Calway, T., Wang, Y., Yuan, C., Wang, C."Pretreatment of baicalin and wogonoside with glycoside hydrolase: A promising approach to enhance anticancer potential". Oncology Reports 30, no. 5 (2013): 2411-2418. https://doi.org/10.3892/or.2013.2726
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