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Article

Correlation and prognostic value of osteopontin and Bcl-2 in hepatocellular carcinoma patients after curative resection

  • Authors:
    • Biao Deng
    • Xiao-Fei Zhang
    • Xu-Chao Zhu
    • Hua Huang
    • Hu-Liang Jia
    • Qing-Hai Ye
    • Qiong-Zhu Dong
    • Lun-Xiu Qin
  • View Affiliations / Copyright

    Affiliations: Liver Cancer Institute and Zhongshan Hospital, Institutes of Biomedical Sciences, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai 200032, P.R. China
  • Pages: 2795-2803
    |
    Published online on: September 19, 2013
       https://doi.org/10.3892/or.2013.2737
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Abstract

Osteopontin (OPN) may facilitate tumorigenesis and metastasis through prevention of tumor cells from apoptosis. Although previous studies have suggested involvement of enhanced Bcl-2 protein family expression, the role of OPN together with Bcl-2 in hepatocellular carcinoma (HCC) remains unknown. In this study, we used western blotting to detect the OPN and Bcl-2 expression levels in cell lines with different OPN backgrounds and HCC tissues, and tumor tissue microarrays to examine OPN and Bcl-2 expression levels in 454 HCC cases. The Kaplan-Meier method and log-rank test were applied to investigate the predictive values of OPN and Bcl-2 in HCC patients. In vitro assays indicated that OPN expression increased concordantly with increasing metastatic potential in MHCC97-H, MHCC97-L, HepG2 and SMMC-7721 cell lines by western blotting, whereas Bcl-2 expression declined. In addition, Bcl-2 was highly upregulated in OPN knockdown MHCC97-H cell lines. Furthermore, in HCC tissues, it was confirmed that OPN levels were also significantly higher in recurrent tumor tissues compared to non-recurrent tissues by western blotting (p<0.001), whereas the contrary occurred in Bcl-2 (p=0.046). Using immunohistochemistry analysis, patients with higher OPN levels had significantly shorter median survival time and recurrence time compared to the lower ones, although the opposite occurred in Bcl-2 levels. Of note, when OPN and Bcl-2 were combined, we found that the co-index of OPN/Bcl-2 was an independent prognostic factor for both overall survival (p<0.001) and time to recurrence (p<0.001). Our findings demonstrate that OPN/Bcl-2 expression is a promising independent predictor of recurrence and survival in HCC. Additionally, Bcl-2 levels may be regulated by OPN in the HCC microenvironment.
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Copy and paste a formatted citation
Spandidos Publications style
Deng B, Zhang X, Zhu X, Huang H, Jia H, Ye Q, Dong Q and Qin L: Correlation and prognostic value of osteopontin and Bcl-2 in hepatocellular carcinoma patients after curative resection. Oncol Rep 30: 2795-2803, 2013.
APA
Deng, B., Zhang, X., Zhu, X., Huang, H., Jia, H., Ye, Q. ... Qin, L. (2013). Correlation and prognostic value of osteopontin and Bcl-2 in hepatocellular carcinoma patients after curative resection. Oncology Reports, 30, 2795-2803. https://doi.org/10.3892/or.2013.2737
MLA
Deng, B., Zhang, X., Zhu, X., Huang, H., Jia, H., Ye, Q., Dong, Q., Qin, L."Correlation and prognostic value of osteopontin and Bcl-2 in hepatocellular carcinoma patients after curative resection". Oncology Reports 30.6 (2013): 2795-2803.
Chicago
Deng, B., Zhang, X., Zhu, X., Huang, H., Jia, H., Ye, Q., Dong, Q., Qin, L."Correlation and prognostic value of osteopontin and Bcl-2 in hepatocellular carcinoma patients after curative resection". Oncology Reports 30, no. 6 (2013): 2795-2803. https://doi.org/10.3892/or.2013.2737
Copy and paste a formatted citation
x
Spandidos Publications style
Deng B, Zhang X, Zhu X, Huang H, Jia H, Ye Q, Dong Q and Qin L: Correlation and prognostic value of osteopontin and Bcl-2 in hepatocellular carcinoma patients after curative resection. Oncol Rep 30: 2795-2803, 2013.
APA
Deng, B., Zhang, X., Zhu, X., Huang, H., Jia, H., Ye, Q. ... Qin, L. (2013). Correlation and prognostic value of osteopontin and Bcl-2 in hepatocellular carcinoma patients after curative resection. Oncology Reports, 30, 2795-2803. https://doi.org/10.3892/or.2013.2737
MLA
Deng, B., Zhang, X., Zhu, X., Huang, H., Jia, H., Ye, Q., Dong, Q., Qin, L."Correlation and prognostic value of osteopontin and Bcl-2 in hepatocellular carcinoma patients after curative resection". Oncology Reports 30.6 (2013): 2795-2803.
Chicago
Deng, B., Zhang, X., Zhu, X., Huang, H., Jia, H., Ye, Q., Dong, Q., Qin, L."Correlation and prognostic value of osteopontin and Bcl-2 in hepatocellular carcinoma patients after curative resection". Oncology Reports 30, no. 6 (2013): 2795-2803. https://doi.org/10.3892/or.2013.2737
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