REG Iα gene expression is linked with the poor prognosis of lung adenocarcinoma and squamous cell carcinoma patients via discrete mechanisms

Kimura,Michitaka; Naito,Hiroshi; Tojo,Takashi; Itaya-Hironaka,Asako; Dohi,Yoshiko; Yoshimura,Mamiko; Nakagawara,Kan-Ichi; Takasawa,Shin; Taniguchi,Shigeki

doi:10.3892/or.2013.2739

REG Iα gene expression is linked with the poor prognosis of lung adenocarcinoma and squamous cell carcinoma patients via discrete mechanisms

Authors:
- Michitaka Kimura
- Hiroshi Naito
- Takashi Tojo
- Asako Itaya-Hironaka
- Yoshiko Dohi
- Mamiko Yoshimura
- Kan-Ichi Nakagawara
- Shin Takasawa
- Shigeki Taniguchi
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Affiliations: Department of Thoracic and Cardiovascular Surgery, Nara Medical University, Kashihara, Nara 634-8522, Japan, Department of Biochemistry, Nara Medical University, Kashihara, Nara 634-8522, Japan, Nihon Gene Research Laboratories Inc., Sendai, Miyagi 983-0005, Japan
Published online on: September 19, 2013 https://doi.org/10.3892/or.2013.2739
Pages: 2625-2631
Copyright: © Kimura et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The aim of the present study was to evaluate the effects of the REG Iα and REG Iβ genes on lung cancer cell lines, and thereafter, the expression of REG family genes (REG Iα, REG Iβ, REG III, HIP/PAP and REG IV) in lung cancer in relation to patient prognosis was evaluated. Lung adenocarcinoma (AD) and squamous cell carcinoma (SCC) cell lines expressing REG Iα or REG Iβ (HLC-1 REG Iα/Iβ and EBC-1 REG Iα/Iβ) were established, and cell number, cell invasive activity, and anchorage-independent cell growth were compared with these variables in the control cells. The expression levels of REG family genes were evaluated by real-time RT-PCR in surgically resected lung cancers, and disease-specific survival (DSS) curves were generated. The HLC-1 REG Iα/Iβ cell line showed significant increases in cell number and anchorage-independent cell growth compared with the control cells. EBC-1 REG Iα/Iβ cells showed significant increases in cell invasive activity and anchorage-independent cell growth as compared with the control cells. Except for the REG Iβ gene, expression of other REG family genes was observed in the surgically resected samples; however, DSS was significantly worse only in stage I patients who were positive for REG Iα expression than in patients who were negative for REG Iα expression. The effects of REG Iα on AD and SCC cells were different in the in vitro study, and a correlation between REG Iα expression and patient prognosis was noted in the in vivo study. Therefore, overexpression of REG Iα is a risk factor for poor prognosis caused by discrete mechanisms in AD and SCC patients.

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