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Article

14-3-3β regulates the proliferation of glioma cells through the GSK3β/β-catenin signaling pathway

  • Authors:
    • Fanghe Gong
    • Guoliang Wang
    • Jing Ye
    • Tiandong Li
    • Hongmin Bai
    • Weimin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, P.R. China, Department of Huiqiao, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P.R. China
  • Pages: 2976-2982
    |
    Published online on: September 19, 2013
       https://doi.org/10.3892/or.2013.2740
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Abstract

We previously demonstrated that 14-3-3β is overexpressed in astrocytomas; however, the underlying mechanisms are poorly understood. Based on the reported multiple functions of 14-3-3β, we hypothesized that it interacts with glycogen synthase kinase 3 β (GSK3β), which regulates β-catenin-mediated oncogene expression and contributes to tumorigenesis and astrocytoma progression. To test these hypotheses, we used 14-3-3β overexpression vectors and small interfering RNA (siRNA) transfection in the human normal astrocyte cell line SVGp12 and the glioma cell line U87, respectively. The results showed that overexpression of 14-3-3β promoted the proliferation of SVGp12 cells, while knockdown of 14-3-3β inhibited the proliferation of U87 cells as analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and bromodeoxyuridine (BrdU) assays. In Flag-tagged 14-3-3β-overexpressing cells, GSK3β was co-immunoprecipitated with 14-3-3β using a Flag antibody. Knockdown of β-catenin by siRNA blocked cell proliferation induced by overexpression of 14-3-3β. Furthermore, overexpression of 14-3-3β suppressed the phosphorylation of β-catenin leading to its accumulation and nuclear translocation as revealed by western blot analysis. In addition, β-catenin nuclear translocation induced by overexpression of 14-3-3β activated the transcription of oncogenes including c-myc and cyclin D1. Collectively, these results revealed that 14-3-3β regulates the proliferation of astrocytes and glioma cells through the GSK3β/β-catenin signaling pathway. The delineated mechanism of 14-3-3β may be responsible for the tumorigenesis and progression of human astrocytomas. Thus, new therapeutic strategies or drugs aimed at 14-3-3β may have potential for the treatment of human astrocytomas.
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Copy and paste a formatted citation
Spandidos Publications style
Gong F, Wang G, Ye J, Li T, Bai H and Wang W: 14-3-3β regulates the proliferation of glioma cells through the GSK3β/β-catenin signaling pathway. Oncol Rep 30: 2976-2982, 2013.
APA
Gong, F., Wang, G., Ye, J., Li, T., Bai, H., & Wang, W. (2013). 14-3-3β regulates the proliferation of glioma cells through the GSK3β/β-catenin signaling pathway. Oncology Reports, 30, 2976-2982. https://doi.org/10.3892/or.2013.2740
MLA
Gong, F., Wang, G., Ye, J., Li, T., Bai, H., Wang, W."14-3-3β regulates the proliferation of glioma cells through the GSK3β/β-catenin signaling pathway". Oncology Reports 30.6 (2013): 2976-2982.
Chicago
Gong, F., Wang, G., Ye, J., Li, T., Bai, H., Wang, W."14-3-3β regulates the proliferation of glioma cells through the GSK3β/β-catenin signaling pathway". Oncology Reports 30, no. 6 (2013): 2976-2982. https://doi.org/10.3892/or.2013.2740
Copy and paste a formatted citation
x
Spandidos Publications style
Gong F, Wang G, Ye J, Li T, Bai H and Wang W: 14-3-3β regulates the proliferation of glioma cells through the GSK3β/β-catenin signaling pathway. Oncol Rep 30: 2976-2982, 2013.
APA
Gong, F., Wang, G., Ye, J., Li, T., Bai, H., & Wang, W. (2013). 14-3-3β regulates the proliferation of glioma cells through the GSK3β/β-catenin signaling pathway. Oncology Reports, 30, 2976-2982. https://doi.org/10.3892/or.2013.2740
MLA
Gong, F., Wang, G., Ye, J., Li, T., Bai, H., Wang, W."14-3-3β regulates the proliferation of glioma cells through the GSK3β/β-catenin signaling pathway". Oncology Reports 30.6 (2013): 2976-2982.
Chicago
Gong, F., Wang, G., Ye, J., Li, T., Bai, H., Wang, W."14-3-3β regulates the proliferation of glioma cells through the GSK3β/β-catenin signaling pathway". Oncology Reports 30, no. 6 (2013): 2976-2982. https://doi.org/10.3892/or.2013.2740
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