Mature BDNF promotes the growth of glioma cells in vitro

Xiong,Jing; Zhou,Li; Lim,Yoon; Yang,Miao; Zhu,Yu-Hong; Li,Zhi-Wei; Zhou,Fiona   H.; Xiao,Zhi-Cheng; Zhou,Xin-Fu

doi:10.3892/or.2013.2746

Mature BDNF promotes the growth of glioma cells in vitro

Authors:
- Jing Xiong
- Li Zhou
- Yoon Lim
- Miao Yang
- Yu-Hong Zhu
- Zhi-Wei Li
- Fiona H. Zhou
- Zhi-Cheng Xiao
- Xin-Fu Zhou
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Affiliations: Key Laboratory of Stem Cells and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan, P.R. China, School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia, The Second Hospital Affiliated to Kunming Medical University, Kunming, Yunnan, P.R. China, Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC 3800, Australia
Published online on: September 20, 2013 https://doi.org/10.3892/or.2013.2746
Pages: 2719-2724

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Abstract

High-grade glioma is incurable and is associated with a short survival time and a poor prognosis. There are two forms of brain-derived neurotrophic factor (BDNF), proBDNF and mature BDNF, which exert opposite effects. Their diverse actions are mediated through two different transmembrane receptor signalling systems: p75NTR and TrkB. The important roles of the BDNF/TrkB signalling system in tumour cell proliferation and survival have been demonstrated. However, few studies have been able to distinguish mature BDNF from proBDNF due to the limitation of specific antibodies. Using specific proBDNF antibodies, we demonstrated that the proBDNF/p75NTR pathway appears to inhibit malignant glioma cell growth and migration. In the present study using specific mature BDNF antibodies, we found that mature BDNF inhibited C6 glioma cell apoptosis and increased cell growth and migration in vitro. Our data suggest that the counterbalance between mature BDNF and proBDNF may regulate tumour growth.

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