miR-205 promotes the growth, metastasis and chemoresistance of NSCLC cells by targeting PTEN

Lei,Lin; Huang,Yaping; Gong,Wenrong

doi:10.3892/or.2013.2755

miR-205 promotes the growth, metastasis and chemoresistance of NSCLC cells by targeting PTEN

Authors:
- Lin Lei
- Yaping Huang
- Wenrong Gong
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Affiliations: Department of Oncology, Xiangyang Central Hospital, Xiangyang 441021, P.R. China, Department of Respiratory Medicine, Xiangyang Central Hospital, Xiangyang 441021, P.R. China, Institute of Oncology, Medical College of Hubei University of Arts and Science, Xiangyang, Hubei 441053, P.R. China
Published online on: September 30, 2013 https://doi.org/10.3892/or.2013.2755
Pages: 2897-2902

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Abstract

Non-small cell lung cancer (NSCLC) is one of the most common causes of cancer-related mortality worldwide. microRNAs (miRNAs) play critical roles in carcinogenesis. miR-205 has been shown to be upregulated in NSCLC. In the present study, we identified the promotive effects of miR-205 on various significant biological properties of NSCLC cells, and confirmed the regulation of PTEN by miR-205. The expression of miR-205 was examined by quantitative real-time PCR both in NSCLC cell lines and tissues. The effect of miR-205 on PTEN expression was assessed in NSCLC cell lines with miR-205 mimics/inhibitor to elevate/decrease miR-205 expression. Furthermore, the roles of miR-205 in regulating the biological properties of NSCLC cells, including growth, invasion and chemoresistance, were assayed using miR-205 mimic/inhibitor-transfected cells. The 3'-untranslated region (3'-UTR) of PTEN combined with miR-205 and this was confirmed by luciferase reporter assay and western blotting. miR-205 expression was increased in NSCLC cell lines as well as in tissues. Overexpression of miR-205 promoted growth, migration and invasion, and enhanced the chemoresistance of NSCLC cells. Luciferase activity and western blotting demonstrated that miR-205 negatively regulated PTEN at a posttranscriptional level. However, miR-205 knockdown suppressed these processes in A549 cells and increased the expression of PTEN protein. Furthermore, overexpression of PTEN exhibited effects identical with those of the miR-205 inhibitor in NSCLC cells. Our results demonstrated that miR-205 is involved in the tumorigenesis of NSCLC through modulation of the PTEN signaling pathway.

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