Etoposide induces apoptosis via the mitochondrial- and caspase-dependent pathways and in non-cancer stem cells in Panc-1 pancreatic cancer cells

  • Authors:
    • She-Hong Zhang
    • Qian Huang
  • View Affiliations

  • Published online on: October 1, 2013     https://doi.org/10.3892/or.2013.2767
  • Pages: 2765-2770
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Abstract

Pancreatic cancer is a highly aggressive malignant tumor. In the present study, we performed several methods, including CCK-8 assay, immunofluorescence technique, western blotting and flow cytometry, to determine the effects of VP16 (etoposide) on Panc-1 pancreatic cancer cells. The results demonstrated that VP16 inhibited the growth of and induced apoptosis in Panc-1 cells. Western blot analysis showed that VP16 inhibited the expression of Bcl-2 and enhanced the expression of Bax, caspases-3 and -9, cytochrome c and PARP. Notably, a strong inhibitory effect of VP16 on Panc-1 cells mainly occurred in non-CSCs. These data provide a new strategy for the therapy of pancreatic cancer.
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December 2013
Volume 30 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang S and Zhang S: Etoposide induces apoptosis via the mitochondrial- and caspase-dependent pathways and in non-cancer stem cells in Panc-1 pancreatic cancer cells. Oncol Rep 30: 2765-2770, 2013
APA
Zhang, S., & Zhang, S. (2013). Etoposide induces apoptosis via the mitochondrial- and caspase-dependent pathways and in non-cancer stem cells in Panc-1 pancreatic cancer cells. Oncology Reports, 30, 2765-2770. https://doi.org/10.3892/or.2013.2767
MLA
Zhang, S., Huang, Q."Etoposide induces apoptosis via the mitochondrial- and caspase-dependent pathways and in non-cancer stem cells in Panc-1 pancreatic cancer cells". Oncology Reports 30.6 (2013): 2765-2770.
Chicago
Zhang, S., Huang, Q."Etoposide induces apoptosis via the mitochondrial- and caspase-dependent pathways and in non-cancer stem cells in Panc-1 pancreatic cancer cells". Oncology Reports 30, no. 6 (2013): 2765-2770. https://doi.org/10.3892/or.2013.2767