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Article

Identification of CD90 as a marker for lung cancer stem cells in A549 and H446 cell lines

  • Authors:
    • Xiuping Yan
    • Hu Luo
    • Xiangdong Zhou
    • Bingjing Zhu
    • Yuliang Wang
    • Xiuwu Bian
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The First Affiliated Hospital of Third Military Medical University, Chongqing 400038, P.R. China, Institute of Pathology and Southwest Cancer Center, The First Affiliated Hospital of Third Military Medical University, Chongqing 400038, P.R. China
  • Pages: 2733-2740
    |
    Published online on: October 3, 2013
       https://doi.org/10.3892/or.2013.2784
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Abstract

Accumulating evidence supports that cancer stem cells (CSCs) are responsible for tumor initiation, progression, distal metastasis and even drug resistance. Although CD90 has been identified as a marker for several types of stem cells, such as liver CSCs, the potential role of CD90 as a marker for lung CSCs has yet to be fully characterized. Our previous study demonstrated that the lung cancer stem-like cells isolated from A549 tumor spheres, which were cultured in serum-free conditioned medium, had stronger proliferation and self-renewal abilities, and expressed higher levels of the stem cell markers Sox2 and Oct4 as compared to A549 adherent cells. In the present study, we identified CD90 as a novel surface marker of CSCs in lung cancer cells. Furthermore, we isolated CD90+ CSCs from lung cancer cell lines A549 and H446. Our results revealed that the CD90+ cells, but not the CD90- cells, from lung cancer cells displayed higher tumorigenic capacity. These findings suggest that CD90 could be a potential marker of lung CSCs and thus provide new insight into further therapeutic strategies of lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Yan X, Luo H, Zhou X, Zhu B, Wang Y and Bian X: Identification of CD90 as a marker for lung cancer stem cells in A549 and H446 cell lines. Oncol Rep 30: 2733-2740, 2013.
APA
Yan, X., Luo, H., Zhou, X., Zhu, B., Wang, Y., & Bian, X. (2013). Identification of CD90 as a marker for lung cancer stem cells in A549 and H446 cell lines. Oncology Reports, 30, 2733-2740. https://doi.org/10.3892/or.2013.2784
MLA
Yan, X., Luo, H., Zhou, X., Zhu, B., Wang, Y., Bian, X."Identification of CD90 as a marker for lung cancer stem cells in A549 and H446 cell lines". Oncology Reports 30.6 (2013): 2733-2740.
Chicago
Yan, X., Luo, H., Zhou, X., Zhu, B., Wang, Y., Bian, X."Identification of CD90 as a marker for lung cancer stem cells in A549 and H446 cell lines". Oncology Reports 30, no. 6 (2013): 2733-2740. https://doi.org/10.3892/or.2013.2784
Copy and paste a formatted citation
x
Spandidos Publications style
Yan X, Luo H, Zhou X, Zhu B, Wang Y and Bian X: Identification of CD90 as a marker for lung cancer stem cells in A549 and H446 cell lines. Oncol Rep 30: 2733-2740, 2013.
APA
Yan, X., Luo, H., Zhou, X., Zhu, B., Wang, Y., & Bian, X. (2013). Identification of CD90 as a marker for lung cancer stem cells in A549 and H446 cell lines. Oncology Reports, 30, 2733-2740. https://doi.org/10.3892/or.2013.2784
MLA
Yan, X., Luo, H., Zhou, X., Zhu, B., Wang, Y., Bian, X."Identification of CD90 as a marker for lung cancer stem cells in A549 and H446 cell lines". Oncology Reports 30.6 (2013): 2733-2740.
Chicago
Yan, X., Luo, H., Zhou, X., Zhu, B., Wang, Y., Bian, X."Identification of CD90 as a marker for lung cancer stem cells in A549 and H446 cell lines". Oncology Reports 30, no. 6 (2013): 2733-2740. https://doi.org/10.3892/or.2013.2784
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