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Androgen receptor is negatively correlated with the methylation-mediated transcriptional repression of miR-375 in human prostate cancer cells

  • Authors:
    • Mingliang Chu
    • Yunli Chang
    • Ping Li
    • Yanjing Guo
    • Kaiqing Zhang
    • Weiqiang Gao
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Oncogenes and Related Genes, Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China
    Copyright: © Chu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 34-40
    |
    Published online on: October 24, 2013
       https://doi.org/10.3892/or.2013.2810
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Abstract

Androgen receptor (AR) plays a critical role during the development and progression of prostate cancer in which microRNA miR-375 is overexpressed and correlated with tumor progression. Although DNA methylation is a key mechanism for the repression of gene expression, the relationship between AR and the expression or the hypermethylation of miR-375 is unknown. In this study, we found that AR-positive prostate cancer (PCa) cells showed high expression levels and hypomethylation of the miR-375. In contrast, AR-negative PCa cells displayed low levels and hypermethylation of the miR-375. Addition of 5-Aza-2'-deoxycytidine, a specific inhibitor of DNA methylation, into the culture medium reversed the low expression levels of miR-375 in the AR negative PCa cells. In addition, the total activity levels of DNA methyltransferases (DNMTs) were high in AR-negative PCa cells, in which hypermethylation of miR-375 promoter and low expression levels of miR-375 were observed. Taken together, these findings indicate that the negative correlation between AR and total DNMT activity is one of mechanisms to influence the methylation status of miR-375 promoter, which in turn regulates the expression of miR-375.
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Copy and paste a formatted citation
Spandidos Publications style
Chu M, Chang Y, Li P, Guo Y, Zhang K and Gao W: Androgen receptor is negatively correlated with the methylation-mediated transcriptional repression of miR-375 in human prostate cancer cells. Oncol Rep 31: 34-40, 2014.
APA
Chu, M., Chang, Y., Li, P., Guo, Y., Zhang, K., & Gao, W. (2014). Androgen receptor is negatively correlated with the methylation-mediated transcriptional repression of miR-375 in human prostate cancer cells. Oncology Reports, 31, 34-40. https://doi.org/10.3892/or.2013.2810
MLA
Chu, M., Chang, Y., Li, P., Guo, Y., Zhang, K., Gao, W."Androgen receptor is negatively correlated with the methylation-mediated transcriptional repression of miR-375 in human prostate cancer cells". Oncology Reports 31.1 (2014): 34-40.
Chicago
Chu, M., Chang, Y., Li, P., Guo, Y., Zhang, K., Gao, W."Androgen receptor is negatively correlated with the methylation-mediated transcriptional repression of miR-375 in human prostate cancer cells". Oncology Reports 31, no. 1 (2014): 34-40. https://doi.org/10.3892/or.2013.2810
Copy and paste a formatted citation
x
Spandidos Publications style
Chu M, Chang Y, Li P, Guo Y, Zhang K and Gao W: Androgen receptor is negatively correlated with the methylation-mediated transcriptional repression of miR-375 in human prostate cancer cells. Oncol Rep 31: 34-40, 2014.
APA
Chu, M., Chang, Y., Li, P., Guo, Y., Zhang, K., & Gao, W. (2014). Androgen receptor is negatively correlated with the methylation-mediated transcriptional repression of miR-375 in human prostate cancer cells. Oncology Reports, 31, 34-40. https://doi.org/10.3892/or.2013.2810
MLA
Chu, M., Chang, Y., Li, P., Guo, Y., Zhang, K., Gao, W."Androgen receptor is negatively correlated with the methylation-mediated transcriptional repression of miR-375 in human prostate cancer cells". Oncology Reports 31.1 (2014): 34-40.
Chicago
Chu, M., Chang, Y., Li, P., Guo, Y., Zhang, K., Gao, W."Androgen receptor is negatively correlated with the methylation-mediated transcriptional repression of miR-375 in human prostate cancer cells". Oncology Reports 31, no. 1 (2014): 34-40. https://doi.org/10.3892/or.2013.2810
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