SC35 promotes splicing of the C5-V6-C6 isoform of CD44 pre-mRNA

  • Authors:
    • Tiing Jen Loh
    • Heegyum Moon
    • Sunghee Cho
    • Da-Woon Jung
    • Seong-Eui Hong
    • Do Han Kim
    • Michael R. Green
    • Xuexiu Zheng
    • Jianhua Zhou
    • Haihong Shen
  • View Affiliations

  • Published online on: October 24, 2013     https://doi.org/10.3892/or.2013.2812
  • Pages: 273-279
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Abstract

CD44 is a cell membrane glycoprotein that mediates the response of cells to their cellular microenvironment and regulates growth, survival, differentiation and motility. CD44 pre-mRNA contains 20 exons, 10 of which are alternatively spliced. Among the CD44 spliced variants, one of the V6 exon-containing isoforms, the V4-7 variant which contains variable exons 4, 5, 6 and 7, confers metastatic potential to non-metastatic cells. However, the splicing regulation of the V6 exon is not completely understood. SC35 is an arginine-serine rich protein that regulates alternative splicing of various pre-mRNAs. In the present study, we established a stable cell line which indicates inclusion or skipping of the V6 exon with the RFP or GFP signal. Using this stable cell line, we found that the V6 exon and flanking introns of CD44 pre-mRNA contained SC35 response elements that regulate V6 splicing. RT-PCR analyses of the endogenous CD44 splicing showed that SC35 promotes the production of the C5-V6-C6 isoform. shRNA knockdown of SC35 showed that reduced expression of SC35 decreased expression of the V6 exon-containing isoforms. Our results reveal a novel mechanism of CD44V6 splicing.
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2014-January
Volume 31 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Loh TJ, Moon H, Cho S, Jung D, Hong S, Kim DH, Green MR, Zheng X, Zhou J, Shen H, Shen H, et al: SC35 promotes splicing of the C5-V6-C6 isoform of CD44 pre-mRNA. Oncol Rep 31: 273-279, 2014
APA
Loh, T.J., Moon, H., Cho, S., Jung, D., Hong, S., Kim, D.H. ... Shen, H. (2014). SC35 promotes splicing of the C5-V6-C6 isoform of CD44 pre-mRNA. Oncology Reports, 31, 273-279. https://doi.org/10.3892/or.2013.2812
MLA
Loh, T. J., Moon, H., Cho, S., Jung, D., Hong, S., Kim, D. H., Green, M. R., Zheng, X., Zhou, J., Shen, H."SC35 promotes splicing of the C5-V6-C6 isoform of CD44 pre-mRNA". Oncology Reports 31.1 (2014): 273-279.
Chicago
Loh, T. J., Moon, H., Cho, S., Jung, D., Hong, S., Kim, D. H., Green, M. R., Zheng, X., Zhou, J., Shen, H."SC35 promotes splicing of the C5-V6-C6 isoform of CD44 pre-mRNA". Oncology Reports 31, no. 1 (2014): 273-279. https://doi.org/10.3892/or.2013.2812