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Article

Protein-bound polysaccharide-K reduces the proportion of regulatory T cells in vitro and in vivo

  • Authors:
    • Rieko Aoki
    • Hiroko Iijima
    • Mariko Kato
    • Motoyuki Uchida
    • Tsutomu Wada
    • Masatsune Murata
    • Kenji Ogawa
    • Yoshihiko Naritaka
    • Kazuhiko Yoshimatsu
  • View Affiliations / Copyright

    Affiliations: Pharmaceuticals Division, Kureha Corporation, Tokyo, Japan, Department of Nutrition and Food Science, Ochanomizu University, Tokyo, Japan, Department of Surgery, Tokyo Women's Medical University, Medical Center East, Tokyo, Japan
  • Pages: 50-56
    |
    Published online on: November 4, 2013
       https://doi.org/10.3892/or.2013.2834
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Abstract

Regulatory T cells (Tregs) play an important role in maintaining immunological tolerance. However, this mechanism is one of the major obstacles to overcome when attempting to improve antitumor immunity. Protein-bound polysaccharide‑K (PSK) has been used clinically as an antitumor drug, and one of its antitumor mechanisms involves improvement of the tumor-induced immunosuppressive state. Therefore, we investigated whether PSK affects Tregs in vitro and in vivo. In the in vitro study, CD4+CD25- cells were separated from normal mouse spleen and cultured with or without PSK in the presence of TGF-β. Although TGF-β induced CD4+CD25+Foxp3+ Tregs, PSK reduced the proportion of TGF-β-induced Tregs. In the in vivo study, BALB/c mice were injected subcutaneously with methylcholanthrene-induced fibrosarcoma (Meth A) cells on day 0, and were administered PSK (50 mg/kg) intraperitoneally from day 1, three times per week. After 4 weeks, the tumor volume, the proportion of Tregs and the CD8+/Treg ratio in the spleen, plasma TGF-β concentration, and IFN-γ production by spleen cells were measured. PSK significantly reduced tumor growth, the proportion of Tregs in the spleen and the plasma TGF-β concentration, and significantly increased the CD8+/Treg ratio in the spleen and IFN-γ production by spleen cells. The reduction of the TGF-β concentration in blood by PSK appears to decrease the proportion of Tregs in lymphoid organs and to augment antitumor immunity.
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Copy and paste a formatted citation
Spandidos Publications style
Aoki R, Iijima H, Kato M, Uchida M, Wada T, Murata M, Ogawa K, Naritaka Y and Yoshimatsu K: Protein-bound polysaccharide-K reduces the proportion of regulatory T cells in vitro and in vivo. Oncol Rep 31: 50-56, 2014.
APA
Aoki, R., Iijima, H., Kato, M., Uchida, M., Wada, T., Murata, M. ... Yoshimatsu, K. (2014). Protein-bound polysaccharide-K reduces the proportion of regulatory T cells in vitro and in vivo. Oncology Reports, 31, 50-56. https://doi.org/10.3892/or.2013.2834
MLA
Aoki, R., Iijima, H., Kato, M., Uchida, M., Wada, T., Murata, M., Ogawa, K., Naritaka, Y., Yoshimatsu, K."Protein-bound polysaccharide-K reduces the proportion of regulatory T cells in vitro and in vivo". Oncology Reports 31.1 (2014): 50-56.
Chicago
Aoki, R., Iijima, H., Kato, M., Uchida, M., Wada, T., Murata, M., Ogawa, K., Naritaka, Y., Yoshimatsu, K."Protein-bound polysaccharide-K reduces the proportion of regulatory T cells in vitro and in vivo". Oncology Reports 31, no. 1 (2014): 50-56. https://doi.org/10.3892/or.2013.2834
Copy and paste a formatted citation
x
Spandidos Publications style
Aoki R, Iijima H, Kato M, Uchida M, Wada T, Murata M, Ogawa K, Naritaka Y and Yoshimatsu K: Protein-bound polysaccharide-K reduces the proportion of regulatory T cells in vitro and in vivo. Oncol Rep 31: 50-56, 2014.
APA
Aoki, R., Iijima, H., Kato, M., Uchida, M., Wada, T., Murata, M. ... Yoshimatsu, K. (2014). Protein-bound polysaccharide-K reduces the proportion of regulatory T cells in vitro and in vivo. Oncology Reports, 31, 50-56. https://doi.org/10.3892/or.2013.2834
MLA
Aoki, R., Iijima, H., Kato, M., Uchida, M., Wada, T., Murata, M., Ogawa, K., Naritaka, Y., Yoshimatsu, K."Protein-bound polysaccharide-K reduces the proportion of regulatory T cells in vitro and in vivo". Oncology Reports 31.1 (2014): 50-56.
Chicago
Aoki, R., Iijima, H., Kato, M., Uchida, M., Wada, T., Murata, M., Ogawa, K., Naritaka, Y., Yoshimatsu, K."Protein-bound polysaccharide-K reduces the proportion of regulatory T cells in vitro and in vivo". Oncology Reports 31, no. 1 (2014): 50-56. https://doi.org/10.3892/or.2013.2834
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