Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
2014-January Volume 31 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-January Volume 31 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Aberrant SERCA3 expression during the colorectal adenoma-adenocarcinoma sequence

  • Authors:
    • Wen-Feng Gou
    • Zhe-Feng Niu
    • Shuang Zhao
    • Yasuo Takano
    • Hua-Chuan Zheng
  • View Affiliations / Copyright

    Affiliations: Cancer Research Center, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China, Clinical Research Institute, Kanagawa Cancer Center, Yokohama 241-0815, Japan
  • Pages: 232-240
    |
    Published online on: November 7, 2013
       https://doi.org/10.3892/or.2013.2837
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) 3 is involved in calcium mobilization from the endoplasmic reticulum into the cytosol and is closely linked to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding and carcinogenesis. In order to elucidate the role of SERCA3 in colorectal carcinogenesis and subsequent progression, its expression was examined using immunohistochemistry and in situ hybridization (ISH) on tissue microarrays containing colorectal carcinomas, adjacent non-neoplastic mucosa (NNM) and adenoma, and metastatic carcinoma in lymph node and liver. Colorectal carcinoma tissue and cell lines were assessed for SERCA3 expression by western blotting or RT-PCR, respectively. SERCA3 was distinctively expressed in Colo201, Colo205, DLD-1, HCT-15, HCT-116, HT-29, KM-12, SW480, SW620 and WiDr cells at both the mRNA and protein levels. SERCA3 mRNA expression was low in carcinoma when compared to that in matched NNM by quantitative PCR (P<0.05), while the converse was true by ISH. Lower expression of SERCA3 was immunohistochemically observed in colorectal carcinoma when compared to that in NNM and adenoma (P<0.05). In contrast, primary carcinoma showed high SERCA3 expression when compared to that in metastatic carcinoma in lymph node or liver by IHC (P<0.05). Immunohistochemically, SERCA3 expression was negatively related to lymphatic invasion, but not with age, gender, depth of invasion, venous invasion, lymph node metastasis, distant metastasis, TNM stage, degree of differentiation or survival rate (P>0.05). There was a positive relationship between SERCA3 expression and serum CEA levels in the carcinoma patients (P<0.05). Cox's proportional hazards model indicated that depth of invasion and distant metastasis are independent prognostic factors for overall colorectal carcinomas (P<0.05). These findings suggest that aberrant SERCA3 expression is closely linked to the adenoma-adenocarcinoma sequence and progression of colorectal carcinomas.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Hovnanian A: SERCA pumps and human diseases. Subcell Biochem. 45:337–363. 2007. View Article : Google Scholar

2 

Wuytack F, Dode L, Baba-Aissa F, et al: The SERCA3-type of organellar Ca2+ pumps. Biosci Rep. 15:299–306. 1995. View Article : Google Scholar : PubMed/NCBI

3 

Baba-Aïssa F, Raeymaekers L, Wuytack F, et al: Purkinje neurons express the SERCA3 isoform of the organellar type Ca(2+)-transport ATPase. Brain Res Mol Brain Res. 41:169–174. 1996.PubMed/NCBI

4 

Chandrasekera PC, Kargacin ME, Deans JP, et al: Determination of apparent calcium affinity for endogenously expressed human sarco(endo)plasmic reticulum calcium-ATPase isoform SERCA3. Am J Physiol Cell Physiol. 296:C1105–C1114. 2009. View Article : Google Scholar

5 

Kao J, Fortner CN, Liu LH, et al: Ablation of the SERCA3 gene alters epithelium-dependent relaxation in mouse tracheal smooth muscle. Am J Physiol Lung Cell Mol Physiol. 277:L264–L270. 1999.PubMed/NCBI

6 

Liu LH, Paul RJ, Sutliff RL, et al: Defective endothelium-dependent relaxation of vascular smooth muscle and endothelial cell Ca2+ signalling in mice lacking sarco(endo)plasmic reticulum Ca2+-ATPase isoform 3. J Biol Chem. 272:30538–30545. 1997. View Article : Google Scholar : PubMed/NCBI

7 

Roe MW, Philipson LH, Frangakis CJ, et al: Defective glucose-dependent endoplasmic reticulum Ca2+ sequestration in diabetic mouse islets of Langerhans. J Biol Chem. 269:18279–18282. 1994.PubMed/NCBI

8 

Varadi A, Lebel L, Hashim Y, et al: Sequence variants of the sarco(endo)plasmic reticulum Ca2+-transport ATPase 3 gene (SERCA3) in Caucasian type II diabetic patients (UK Prospective Diabetes Study 48). Diabetologia. 42:1240–1243. 1999.PubMed/NCBI

9 

Arredouani A, Guiot Y, Jonas JC, et al: SERCA3 ablation does not impair insulin secretion but suggests distinct roles of different sarcoendoplasmic reticulum Ca2+ pumps for Ca2+ homeostasis in pancreatic β-cells. Diabetes. 51:3245–3253. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Gélébart P, Kovács T, Brouland JP, et al: Expression of endomembrane calcium pumps in colon and gastric cancer cells. Induction of SERCA3 expression during differentiation. J Biol Chem. 277:26310–26320. 2002.PubMed/NCBI

11 

Xu XY, Yang X, Takahashi H, et al: The down-regulated SERCA3 expression is closely linked to pathogenesis, invasion, metastasis and poor prognosis of gastric carcinomas. Tumour Biol. 33:1845–1854. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Colorectal Cancer Facts and Figures. American Cancer Society; 2005

13 

Yoshida D, Kono S, Moore MA, et al: Colorectal polypectomy and risk of colorectal cancer by subsite: the Fukuoka Colorectal Cancer study. Jpn J Clin Oncol. 37:597–602. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Hamilton SR and Aaltonen LA: WHO Classification of Tumors: Pathology and Genetics of Tumors of the Digestive System. IARC Press; Lyon: 2000

15 

Sobin LH and Wittekind CH: TNM Classification of Malignant Tumours. 6th edition. John Wiley & Sons; Hoboken, NJ: 2002

16 

Li W, Murai Y, Okada E, et al: Modified and simplified western blotting protocol: use of intermittent microwave irradiation (IMWI) and 5% skim milk to improve binding specificity. Pathol Int. 52:234–238. 2002.PubMed/NCBI

17 

Kumada T, Tsuneyama K, Hatta H, Ishizawa S, et al: Improved 1-h rapid immunostaining method using intermittent microwave irradiation: practicability based on 5 years application in Toyama Medical and Pharmaceutical University Hospital. Mod Pathol. 17:1141–1149. 2004.

18 

Brouland JP, Gélébart P, Kovàcs T, et al: The loss of sarco/endoplasmic reticulum calcium transport ATPase 3 expression is an early event during the multistep process of colon carcinogenesis. Am J Pathol. 167:233–242. 2005. View Article : Google Scholar

19 

Arbabian A, Brouland JP, Gélébart P, et al: Endoplasmic reticulum calcium pumps and cancer. Biofactors. 37:139–149. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Brouland JP, Valleur P and Papp B: Expression of SERCA pumps during cell differentiation and tumorigenesis: application to colonic carcinogenesis. Ann Pathol. 26:159–172. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Petrioli R, Licchetta A, Roviello G, et al: CEA and CA19.9 as early predictors of progression in advanced/metastatic colorectal cancer patients receiving oxaliplatin-based chemotherapy and bevacizumab. Cancer Invest. 3:65–71. 2012. View Article : Google Scholar

22 

Lan YT, Lin JK, Lin TC, et al: Prognostic significance of perioperative change of CEA level in colorectal patients when pre-operative level is normal. Hepatogastroenterology. 59:717–720. 2012.PubMed/NCBI

23 

Lin JK, Lin CC, Yang SH, et al: Early postoperative CEA level is a better prognostic indicator than is preoperative CEA level in predicting prognosis of patients with curable colorectal cancer. Int J Colorectal Dis. 26:1135–1141. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Yeatman TJ, Duan C, Mao W, et al: Augmentation of carcinoembryonic antigen release from intact, viable tumor cells by a factor in human serum. Ann Surg Oncol. 2:336–342. 1995. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Gou W, Niu Z, Zhao S, Takano Y and Zheng H: Aberrant SERCA3 expression during the colorectal adenoma-adenocarcinoma sequence. Oncol Rep 31: 232-240, 2014.
APA
Gou, W., Niu, Z., Zhao, S., Takano, Y., & Zheng, H. (2014). Aberrant SERCA3 expression during the colorectal adenoma-adenocarcinoma sequence. Oncology Reports, 31, 232-240. https://doi.org/10.3892/or.2013.2837
MLA
Gou, W., Niu, Z., Zhao, S., Takano, Y., Zheng, H."Aberrant SERCA3 expression during the colorectal adenoma-adenocarcinoma sequence". Oncology Reports 31.1 (2014): 232-240.
Chicago
Gou, W., Niu, Z., Zhao, S., Takano, Y., Zheng, H."Aberrant SERCA3 expression during the colorectal adenoma-adenocarcinoma sequence". Oncology Reports 31, no. 1 (2014): 232-240. https://doi.org/10.3892/or.2013.2837
Copy and paste a formatted citation
x
Spandidos Publications style
Gou W, Niu Z, Zhao S, Takano Y and Zheng H: Aberrant SERCA3 expression during the colorectal adenoma-adenocarcinoma sequence. Oncol Rep 31: 232-240, 2014.
APA
Gou, W., Niu, Z., Zhao, S., Takano, Y., & Zheng, H. (2014). Aberrant SERCA3 expression during the colorectal adenoma-adenocarcinoma sequence. Oncology Reports, 31, 232-240. https://doi.org/10.3892/or.2013.2837
MLA
Gou, W., Niu, Z., Zhao, S., Takano, Y., Zheng, H."Aberrant SERCA3 expression during the colorectal adenoma-adenocarcinoma sequence". Oncology Reports 31.1 (2014): 232-240.
Chicago
Gou, W., Niu, Z., Zhao, S., Takano, Y., Zheng, H."Aberrant SERCA3 expression during the colorectal adenoma-adenocarcinoma sequence". Oncology Reports 31, no. 1 (2014): 232-240. https://doi.org/10.3892/or.2013.2837
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team