Death associated protein 1 is correlated with the clinical outcome of patients with colorectal cancer and has a role in the regulation of cell death

  • Authors:
    • Yongning Jia
    • Lin Ye
    • Ke Ji
    • Ann-Marie Toms
    • Mansel Leigh Davies
    • Fiona Ruge
    • Jiafu Ji
    • Rachel Hargest
    • Wen G. Jiang
  • View Affiliations

  • Published online on: November 20, 2013     https://doi.org/10.3892/or.2013.2866
  • Pages: 175-182
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Death-associated protein 1 (DAP1) is a member of the DAP family and has been implicated in the regulation of cell growth and death including that of cancer cells. However, the roles of DAP1 in clinical cancer and in the regulation of colorectal cancer cells are largely unknown. The present study investigated the expression profile of DAP1 in human colorectal cancer and the impact of DAP1 on apoptosis and the cellular response to 5-FU. Human colorectal cancer specimens (n=94) and human colorectal cancer cell lines HRT18 and HT115 were used. DAP1 transcript and protein were evaluated using quantitative transcript analysis and immunohistochemistry. DAP1-knockdown cells were generated using anti-DAP1 transgene. The results revealed that human colorectal cancer tissues had lower levels of DAP1 when compared with the normal tissues. The reduced levels were associated with higher Dukes' stage and lymph node metastasis. Patients with low DAP1 expression had a markedly reduced survival. Loss of DAP1 in colorectal cancer cells resulted in a gain in cellular migration and loss of their sensitivity of apoptosis to chemotherapeutic agent, 5-FU. DAP1 was found to be correlated with disease progression and long-term survival of the colorectal patients. DAP1 is also a pivotal regulator of the growth and apoptosis and cellular response to chemotherapy agents.
View Figures
View References

Related Articles

Journal Cover

2014-January
Volume 31 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Jia Y, Ye L, Ji K, Toms A, Davies ML, Ruge F, Ji J, Hargest R and Jiang WG: Death associated protein 1 is correlated with the clinical outcome of patients with colorectal cancer and has a role in the regulation of cell death. Oncol Rep 31: 175-182, 2014
APA
Jia, Y., Ye, L., Ji, K., Toms, A., Davies, M.L., Ruge, F. ... Jiang, W.G. (2014). Death associated protein 1 is correlated with the clinical outcome of patients with colorectal cancer and has a role in the regulation of cell death. Oncology Reports, 31, 175-182. https://doi.org/10.3892/or.2013.2866
MLA
Jia, Y., Ye, L., Ji, K., Toms, A., Davies, M. L., Ruge, F., Ji, J., Hargest, R., Jiang, W. G."Death associated protein 1 is correlated with the clinical outcome of patients with colorectal cancer and has a role in the regulation of cell death". Oncology Reports 31.1 (2014): 175-182.
Chicago
Jia, Y., Ye, L., Ji, K., Toms, A., Davies, M. L., Ruge, F., Ji, J., Hargest, R., Jiang, W. G."Death associated protein 1 is correlated with the clinical outcome of patients with colorectal cancer and has a role in the regulation of cell death". Oncology Reports 31, no. 1 (2014): 175-182. https://doi.org/10.3892/or.2013.2866