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Article

Phosphoglycerate kinase 1 promotes radioresistance in U251 human glioma cells

  • Authors:
    • Hao Ding
    • Yi-Jun Cheng
    • Hua Yan
    • Rui Zhang
    • Jin-Bing Zhao
    • Chun-Fa Qian
    • Wen-Bin Zhang
    • Hong Xiao
    • Hong-Yi Liu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu, P.R. China, Neuro-Psychiatric Institute, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu, P.R. China
  • Pages: 894-900
    |
    Published online on: November 27, 2013
       https://doi.org/10.3892/or.2013.2874
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Abstract

Phosphoglycerate kinase 1 (PGK1) has been found to be increased in radioresistant astrocytomas. The present study was designed to investigate the potential role of PGK1 in the radioresistance in U251 human cells. Quantitative PCR and western blot analysis were performed to evaluate PGK1 expression for mRNA levels and protein levels, respectively. The short hairpin RNA (shRNA)-PGK1 and the high expression plasmids were transfected to radioresistant U251 cells (RR-U251 cells) or normal U251 cells using lipofectamine™ 2000. The cell viability was determined by MTT assay. The wound-healing assay (WHA) was used to evaluate cell migration ability. Cell invasion abilities were examined using a Transwell culture chamber system. Our results showed that the expression of PGK1 was significantly increased in RR-U251 cells compared to normal U251 cells. Following irradiation, the cell viability as well as the migration and invasion ability were significantly higher in RR-U251 cells compared with normal U251 cells. Downregulating PGK1 using shRNA induced a significantly downregulated cell viability and decreased migration and invasion ability, and overexpression of PGK1 contributed to upregulated cell viability and increased migration and invasion ability, both in RR-U251 cells and normal U251 cells. These findings suggest that PGK1 could promote radioresistance in U251 human cells.
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Copy and paste a formatted citation
Spandidos Publications style
Ding H, Cheng Y, Yan H, Zhang R, Zhao J, Qian C, Zhang W, Xiao H and Liu H: Phosphoglycerate kinase 1 promotes radioresistance in U251 human glioma cells. Oncol Rep 31: 894-900, 2014.
APA
Ding, H., Cheng, Y., Yan, H., Zhang, R., Zhao, J., Qian, C. ... Liu, H. (2014). Phosphoglycerate kinase 1 promotes radioresistance in U251 human glioma cells. Oncology Reports, 31, 894-900. https://doi.org/10.3892/or.2013.2874
MLA
Ding, H., Cheng, Y., Yan, H., Zhang, R., Zhao, J., Qian, C., Zhang, W., Xiao, H., Liu, H."Phosphoglycerate kinase 1 promotes radioresistance in U251 human glioma cells". Oncology Reports 31.2 (2014): 894-900.
Chicago
Ding, H., Cheng, Y., Yan, H., Zhang, R., Zhao, J., Qian, C., Zhang, W., Xiao, H., Liu, H."Phosphoglycerate kinase 1 promotes radioresistance in U251 human glioma cells". Oncology Reports 31, no. 2 (2014): 894-900. https://doi.org/10.3892/or.2013.2874
Copy and paste a formatted citation
x
Spandidos Publications style
Ding H, Cheng Y, Yan H, Zhang R, Zhao J, Qian C, Zhang W, Xiao H and Liu H: Phosphoglycerate kinase 1 promotes radioresistance in U251 human glioma cells. Oncol Rep 31: 894-900, 2014.
APA
Ding, H., Cheng, Y., Yan, H., Zhang, R., Zhao, J., Qian, C. ... Liu, H. (2014). Phosphoglycerate kinase 1 promotes radioresistance in U251 human glioma cells. Oncology Reports, 31, 894-900. https://doi.org/10.3892/or.2013.2874
MLA
Ding, H., Cheng, Y., Yan, H., Zhang, R., Zhao, J., Qian, C., Zhang, W., Xiao, H., Liu, H."Phosphoglycerate kinase 1 promotes radioresistance in U251 human glioma cells". Oncology Reports 31.2 (2014): 894-900.
Chicago
Ding, H., Cheng, Y., Yan, H., Zhang, R., Zhao, J., Qian, C., Zhang, W., Xiao, H., Liu, H."Phosphoglycerate kinase 1 promotes radioresistance in U251 human glioma cells". Oncology Reports 31, no. 2 (2014): 894-900. https://doi.org/10.3892/or.2013.2874
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