Expression profiles of 507 proteins from a biotin label-based antibody array in human colorectal cancer

Miyoshi,Hisaaki; Morishita,Asahiro; Tani,Joji; Sakamoto,Teppei; Fujita,Koji; Katsura,Akiko; Tatsuta,Miwa; Nomura,Takako; Yoneyama,Hirohito; Iwama,Hisakazu; Suzuki,Yasuyuki; Masaki,Tsutomu

doi:10.3892/or.2013.2935

2014-March Volume 31 Issue 3

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2014-March Volume 31 Issue 3

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Article

Expression profiles of 507 proteins from a biotin label-based antibody array in human colorectal cancer

Authors:
- Hisaaki Miyoshi
- Asahiro Morishita
- Joji Tani
- Teppei Sakamoto
- Koji Fujita
- Akiko Katsura
- Miwa Tatsuta
- Takako Nomura
- Hirohito Yoneyama
- Hisakazu Iwama
- Yasuyuki Suzuki
- Tsutomu Masaki
View Affiliations / Copyright

Affiliations: Department of Gastroenterology and Neurology, Kagawa University Faculty of Medicine, Kita-gun, Kagawa 761-0793, Japan, Life Science Research Center, Kagawa University Faculty of Medicine, Kita-gun, Kagawa 761-0793, Japan, Department of Gastroenterological Surgery, Kagawa University Faculty of Medicine, Kita-gun, Kagawa 761-0793, Japan
Pages: 1277-1281
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Published online on: December 19, 2013

https://doi.org/10.3892/or.2013.2935
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Abstract

Molecular-targeted therapy is one of the most promising therapies for patients with advanced-stage colorectal cancer (CRC). However, a wide range of proteins have unknown expression levels in CRC. The purpose of the present study was to determine the expression levels of various proteins related to colorectal carcinogenesis and cancer development. We examined the expression levels of 507 target proteins using a biotin label-based antibody array in 6 human CRC tissues. We also analyzed the clinicopathological features of CRC patients. In CRC tissues, IL-1α, GRO, Glut5, MIG, ICAM-5, VE-cadherin, uPA and Leptin R were increased when compared to levels in normal colon tissues. MPIF-1/CCL23, FGF R5, MIP2, SAA and IL-18 Rβ were strongly upregulated in rectal cancer when compared to the levels in non-rectal cancer. These data suggest that differential protein expression profiles exist under different conditions, including carcinogenesis and CRC localization. Therefore, an exhaustive analysis of protein expression levels using a biotin label-based antibody protein array is a potentially useful tool for identifying novel individual therapies for CRC patients.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression profiles of 507 proteins from a biotin label-based antibody array in human colorectal cancer

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Abstract

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