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Article

Protein kinase C-α downregulates estrogen receptor-α by suppressing c-Jun phosphorylation in estrogen receptor-positive breast cancer cells

  • Authors:
    • Sangmin Kim
    • Jeongmin Lee
    • Se Kyung Lee
    • Soo Youn Bae
    • Jiyoung Kim
    • Minkuk Kim
    • Won Ho Kil
    • Seok Won Kim
    • Jeong Eon Lee
    • Seok Jin Nam
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Gangnam-gu, Seoul 135-710, Republic of Korea
  • Pages: 1423-1428
    |
    Published online on: December 19, 2013
       https://doi.org/10.3892/or.2013.2936
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Abstract

Protein kinase C (PKC) activity is elevated in malignant compared with that in normal human breast tissue. In the present study, we investigated the regulatory mechanism and the co-relationship between PKC-α and estrogen receptor-α (ER-α) in ER-α-positive and tamoxifen-resistant (TAMR) breast cancer cells. Our results showed that the level of ER-α expression was significantly decreased in TAMR when compared with that in tamoxifen-sensitive (TAMS) breast cancer cells. However, PKC-α phosphorylation was increased in TAMR breast cancer cells when compared to that in TAMS breast cancer cells. Additionally, ER-α expression was significantly decreased due to the overexpression of constitutively active PKC-α (CA-PKC-α). Next, we investigated the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA), a reversible activator of PKC, on ER-α expression in ER-α-positive breast cancer cells. TPA decreased the levels of ER-α expression in a time- and dose-dependent manner. In contrast, the TPA-induced downregulation of ER-α was prevented by Go6983, a specific PKC inhibitor. Notably, we found that CA-PKC-α suppressed c-JUN phosphorylation, which is a major activating protein-1 factor, and TPA-induced downregulation of ER-α was prevented by SR11302, a specific activator protein-1 inhibitor. Taken together, we demonstrated that PKC-α activity suppressed the level of ER-α expression by inhibiting c-JUN phosphorylation in ER-α-positive breast cancer cells. Therefore, we suggest that PKC-α may be a potential therapeutic target for treating ER-positive and TAMR breast cancer.
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1 

Osborne CK and Schiff R: Estrogen-receptor biology: continuing progress and therapeutic implications. J Clin Oncol. 23:1616–1622. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Osborne CK, Zhao H and Fuqua SA: Selective estrogen receptor modulators: structure, function, and clinical use. J Clin Oncol. 18:3172–3186. 2000.PubMed/NCBI

3 

Harvey JM, Clark GM, Osborne CK and Allred DC: Estrogen receptor status by immunohistochemistry is superior to the ligand-binding assay for predicting response to adjuvant endocrine therapy in breast cancer. J Clin Oncol. 17:1474–1481. 1999.PubMed/NCBI

4 

Herynk MH and Fuqua SA: Estrogen receptors in resistance to hormone therapy. Adv Exp Med Biol. 608:130–143. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Milano A, Dal Lago L, Sotiriou C, Piccart M and Cardoso F: What clinicians need to know about antioestrogen resistance in breast cancer therapy. Eur J Cancer. 42:2692–2705. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Ali S and Coombes RC: Endocrine-responsive breast cancer and strategies for combating resistance. Nat Rev Cancer. 2:101–112. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Kurebayashi J: Endocrine-resistant breast cancer: underlying mechanisms and strategies for overcoming resistance. Breast Cancer. 10:112–119. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Schiff R, Massarweh S, Shou J and Osborne CK: Breast cancer endocrine resistance: how growth factor signaling and estrogen receptor coregulators modulate response. Clin Cancer Res. 9:447S–454S. 2003.PubMed/NCBI

9 

Dekker LV and Parker PJ: Protein kinase C - a question of specificity. Trends Biochem Sci. 19:73–77. 1994. View Article : Google Scholar : PubMed/NCBI

10 

Fabbro D, Kung W, Roos W, Regazzi R and Eppenberger U: Epidermal growth factor binding and protein kinase C activities in human breast cancer cell lines: possible quantitative relationship. Cancer Res. 46:2720–2725. 1986.

11 

Boyan BD, Sylvia VL, Frambach T, Lohmann CH, Dietl J, Dean DD and Schwartz Z: Estrogen-dependent rapid activation of protein kinase C in estrogen receptor-positive MCF-7 breast cancer cells and estrogen receptor-negative HCC38 cells is membrane-mediated and inhibited by tamoxifen. Endocrinology. 144:1812–1824. 2003. View Article : Google Scholar

12 

Gordge PC, Hulme MJ, Clegg RA and Miller WR: Elevation of protein kinase A and protein kinase C activities in malignant as compared with normal human breast tissue. Eur J Cancer. 32A:2120–2126. 1996. View Article : Google Scholar : PubMed/NCBI

13 

Kim S, Han J, Lee SK, Choi MY, Kim J, Lee J, Jung SP, Kim JS, Kim JH, Choe JH, Lee JE and Nam SJ: Berberine suppresses the TPA-induced MMP-1 and MMP-9 expressions through the inhibition of PKC-α in breast cancer cells. J Surg Res. 176:e21–e29. 2012.PubMed/NCBI

14 

Knowlden JM, Hutcheson IR, Jones HE, Madden T, Gee JM, Harper ME, Barrow D, Wakeling AE and Nicholson RI: Elevated levels of epidermal growth factor receptor/c-erbB2 heterodimers mediate an autocrine growth regulatory pathway in tamoxifen-resistant MCF-7 cells. Endocrinology. 144:1032–1044. 2003. View Article : Google Scholar

15 

Hodges RR, Kazlauskas A, Toker A and Dartt DA: Effect of overexpression of protein kinase C alpha on rat lacrimal gland protein secretion. Adv Exp Med Biol. 506:237–241. 2002.PubMed/NCBI

16 

Kikkawa U, Takai Y, Tanaka Y, Miyake R and Nishizuka Y: Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters. J Biol Chem. 258:11442–11445. 1983.PubMed/NCBI

17 

Osborne CK: Tamoxifen in the treatment of breast cancer. N Engl J Med. 339:1609–1618. 1998. View Article : Google Scholar : PubMed/NCBI

18 

Newton AC: Protein kinase C: structure, function, and regulation. J Biol Chem. 270:28495–28498. 1995. View Article : Google Scholar : PubMed/NCBI

19 

Gupta AK, Galoforo SS, Berns CM, Martinez AA, Corry PM, Guan KL and Lee YJ: Elevated levels of ERK2 in human breast carcinoma MCF-7 cells transfected with protein kinase C alpha. Cell Prolif. 29:655–663. 1996. View Article : Google Scholar : PubMed/NCBI

20 

Tonetti DA, O’Regan R, Tanjore S, England G and Jordan VC: Antiestrogen stimulated human endometrial cancer growth: laboratory and clinical considerations. J Steroid Biochem Mol Biol. 65:181–189. 1998. View Article : Google Scholar : PubMed/NCBI

21 

McInerney EM, Weis KE, Sun J, Mosselman S and Katzenellenbogen BS: Transcription activation by the human estrogen receptor subtype β (ERβ) studied with ERβ and ERα receptor chimeras. Endocrinology. 139:4513–4522. 1998.

22 

Basu A: The potential of protein kinase C as a target for anticancer treatment. Pharmacol Ther. 59:257–280. 1993. View Article : Google Scholar : PubMed/NCBI

23 

Blobe GC, Obeid LM and Hannun YA: Regulation of protein kinase C and role in cancer biology. Cancer Metastasis Rev. 13:411–431. 1994. View Article : Google Scholar : PubMed/NCBI

24 

Ways DK, Kukoly CA, deVente J, Hooker JL, Bryant WO, Posekany KJ, Fletcher DJ, Cook PP and Parker PJ: MCF-7 breast cancer cells transfected with protein kinase C-alpha exhibit altered expression of other protein kinase C isoforms and display a more aggressive neoplastic phenotype. J Clin Invest. 95:1906–1915. 1995. View Article : Google Scholar

25 

Kushner PJ, Agard DA, Greene GL, Scanlan TS, Shiau AK, Uht RM and Webb P: Estrogen receptor pathways to AP-1. J Steroid Biochem Mol Biol. 74:311–317. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Jonat C, Rahmsdorf HJ, Park KK, Cato AC, Gebel S, Ponta H and Herrlich P: Antitumor promotion and antiinflammation: down-modulation of AP-1 (Fos/Jun) activity by glucocorticoid hormone. Cell. 62:1189–1204. 1990. View Article : Google Scholar : PubMed/NCBI

27 

Behrens A, Jochum W, Sibilia M and Wagner EF: Oncogenic transformation by ras and fos is mediated by c-Jun N-terminal phosphorylation. Oncogene. 19:2657–2663. 2000. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kim S, Lee J, Lee SK, Bae SY, Kim J, Kim M, Kil WH, Kim SW, Lee JE, Nam SJ, Nam SJ, et al: Protein kinase C-α downregulates estrogen receptor-α by suppressing c-Jun phosphorylation in estrogen receptor-positive breast cancer cells. Oncol Rep 31: 1423-1428, 2014.
APA
Kim, S., Lee, J., Lee, S.K., Bae, S.Y., Kim, J., Kim, M. ... Nam, S.J. (2014). Protein kinase C-α downregulates estrogen receptor-α by suppressing c-Jun phosphorylation in estrogen receptor-positive breast cancer cells. Oncology Reports, 31, 1423-1428. https://doi.org/10.3892/or.2013.2936
MLA
Kim, S., Lee, J., Lee, S. K., Bae, S. Y., Kim, J., Kim, M., Kil, W. H., Kim, S. W., Lee, J. E., Nam, S. J."Protein kinase C-α downregulates estrogen receptor-α by suppressing c-Jun phosphorylation in estrogen receptor-positive breast cancer cells". Oncology Reports 31.3 (2014): 1423-1428.
Chicago
Kim, S., Lee, J., Lee, S. K., Bae, S. Y., Kim, J., Kim, M., Kil, W. H., Kim, S. W., Lee, J. E., Nam, S. J."Protein kinase C-α downregulates estrogen receptor-α by suppressing c-Jun phosphorylation in estrogen receptor-positive breast cancer cells". Oncology Reports 31, no. 3 (2014): 1423-1428. https://doi.org/10.3892/or.2013.2936
Copy and paste a formatted citation
x
Spandidos Publications style
Kim S, Lee J, Lee SK, Bae SY, Kim J, Kim M, Kil WH, Kim SW, Lee JE, Nam SJ, Nam SJ, et al: Protein kinase C-α downregulates estrogen receptor-α by suppressing c-Jun phosphorylation in estrogen receptor-positive breast cancer cells. Oncol Rep 31: 1423-1428, 2014.
APA
Kim, S., Lee, J., Lee, S.K., Bae, S.Y., Kim, J., Kim, M. ... Nam, S.J. (2014). Protein kinase C-α downregulates estrogen receptor-α by suppressing c-Jun phosphorylation in estrogen receptor-positive breast cancer cells. Oncology Reports, 31, 1423-1428. https://doi.org/10.3892/or.2013.2936
MLA
Kim, S., Lee, J., Lee, S. K., Bae, S. Y., Kim, J., Kim, M., Kil, W. H., Kim, S. W., Lee, J. E., Nam, S. J."Protein kinase C-α downregulates estrogen receptor-α by suppressing c-Jun phosphorylation in estrogen receptor-positive breast cancer cells". Oncology Reports 31.3 (2014): 1423-1428.
Chicago
Kim, S., Lee, J., Lee, S. K., Bae, S. Y., Kim, J., Kim, M., Kil, W. H., Kim, S. W., Lee, J. E., Nam, S. J."Protein kinase C-α downregulates estrogen receptor-α by suppressing c-Jun phosphorylation in estrogen receptor-positive breast cancer cells". Oncology Reports 31, no. 3 (2014): 1423-1428. https://doi.org/10.3892/or.2013.2936
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