Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
2014-March Volume 31 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-March Volume 31 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Screening for transcription factors and their regulatory small molecules involved in regulating the functions of CL1-5 cancer cells under the effects of macrophage-conditioned medium

  • Authors:
    • Dongbo Xue
    • Ming Lu
    • Bo Gao
    • Xin Qiao
    • Yingmei Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Surgery, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA, Central Laboratory, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 1323-1333
    |
    Published online on: December 20, 2013
       https://doi.org/10.3892/or.2013.2937
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Many reports have inferred that macrophages can interact with tumor cells in the tumor microenvironment (TME) in a vicious cycle of tumor development; however, the changes in gene expression in tumor cells under the effects of macrophages are still largely unknown. The present study was carried out to illustrate the changes in the gene expression profile in lung cancer cells under the effects of macrophage-conditioned medium. Gene expression profile data were derived from the GEO database GSE9315. The GSM234968 sample was derived from a highly invasive human pulmonary adenocarcinoma cell line, CL1-5, and was treated with conditioned medium (supernatant of a culture solution of human monocyte THP-1). The GSM234967 sample that was not treated with the conditioned medium was used as a control. GO and KEGG enrichment analyses were carried out using DAVID software, and visualization networks were constructed using Cytoscape software. The results showed that 40 differentially expressed genes were annotated. Five differentially expressed transcription factors were identified, EIF2B4, EIF2B5, JUNB, GNG11 and HMGB2, which were all related to ‘stress’ and ‘responses’. The gene cluster of JUNB was mainly enriched in cancer-related pathways, ‘Wnt signaling pathway’ and ‘MAPK signaling pathway’. Finally, 10 small molecules, thioridazine, resveratrol, astemizole, ciclopirox, calmidazolium, etoposide, anisomycin, pyrvinium, azacyclonol and terfenadine, which may act on transcription factors, were identified using the CMap database. In conclusion, we identified transcription factors playing key roles in tumor cells under the effects of macrophages in order to provide new clues for blocking this vicious cycle of tumor development.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Mantovani A, Bottazzi B, Colotta F, Sozzani S and Ruco L: The origin and function of tumor-associated macrophages. Immunol Today. 13:265–270. 1992. View Article : Google Scholar : PubMed/NCBI

2 

Hildenbrand R and Schaaf A: The urokinase-system in tumor tissue stroma of the breast and breast cancer cell invasion. Int J Oncol. 34:15–23. 2009.PubMed/NCBI

3 

Lewis CE and Pollard JW: Distinct role of macrophages in different tumor microenvironments. Cancer Res. 66:605–612. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Gentleman RC, Carey VJ, Bates DM, et al: Bioconductor: open software development for computational biology and bioinformatics. Genome Biol. 5:R802004. View Article : Google Scholar : PubMed/NCBI

5 

Purohit V, Rapaka R, Kwon OS and Song BJ: Roles of alcohol and tobacco exposure in the development of hepatocellular carcinoma. Life Sci. 92:3–9. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Heuff G, Oldenburg HS, Boutkan H, et al: Enhanced tumor growth in the rat liver after selective elimination of Kupffer cells. Cancer Immunol Immunother. 37:125–130. 1993. View Article : Google Scholar : PubMed/NCBI

7 

Chen JJ, Lin YC, Yao PL, et al: Tumor-associated macrophages: the double-edged sword in cancer progression. J Clin Oncol. 23:953–964. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Goetze K, Walenta S, Ksiazkiewicz M, Kunz-Schughart LA and Mueller-Klieser W: Lactate enhances motility of tumor cells and inhibits monocyte migration and cytokine release. Int J Oncol. 39:453–463. 2011.PubMed/NCBI

9 

De Palma M and Lewis CE: Macrophage regulation of tumor responses to anticancer therapies. Cancer Cell. 23:277–286. 2013.PubMed/NCBI

10 

Horton LW, Yu Y, Zaja-Milatovic S, Strieter RM and Richmond A: Opposing roles of murine duffy antigen receptor for chemokine and murine CXC chemokine receptor-2 receptors in murine melanoma tumor growth. Cancer Res. 67:9791–9799. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Smith HA and Kang Y: The metastasis-promoting roles of tumor-associated immune cells. J Mol Med. 91:411–429. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Sawant A, Hensel JA, Chanda D, et al: Depletion of plasmacytoid dendritic cells inhibits tumor growth and prevents bone metastasis of breast cancer cells. J Immunol. 189:4258–4265. 2012. View Article : Google Scholar : PubMed/NCBI

13 

White ES, Strom SR, Wys NL and Arenberg DA: Non-small cell lung cancer cells induce monocytes to increase expression of angiogenic activity. J Immunol. 166:7549–7555. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Lin EY, Li JF, Gnatovskiy L, et al: Macrophages regulate the angiogenic switch in a mouse model of breast cancer. Cancer Res. 66:11238–11246. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Lin EY, Nguyen AV, Russell RG and Pollard JW: Colony-stimulating factor 1 promotes progression of mammary tumors to malignancy. J Exp Med. 193:727–740. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Hagemann T, Robinson SC, Schulz M, et al: Enhanced invasiveness of breast cancer cell lines upon co-cultivation with macrophages is due to TNF-α dependent up-regulation of matrix metalloproteases. Carcinogenesis. 25:1543–1549. 2004.PubMed/NCBI

17 

Andrecht S, Kolbus A, Hartenstein B, Angel P and Schorpp-Kistner M: Cell cycle promoting activity of JunB through cyclin A activation. J Biol Chem. 277:35961–35968. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Schmidt D, Textor B, Pein OT, et al: Critical role for NF-κB-induced JunB in VEGF regulation and tumor angiogenesis. EMBO J. 26:710–719. 2007.

19 

Nausch N, Florin L, Hartenstein B, et al: Cutting edge: the AP-1 subunit JunB determines NK cell-mediated target cell killing by regulation of the NKG2D-ligand RAE-1ɛ. J Immunol. 176:7–11. 2006.PubMed/NCBI

20 

Hussaini IM, Trotter C, Zhao Y, Abdel-Fattah R, et al: Matrix metalloproteinase-9 is differentially expressed in nonfunctioning invasive and noninvasive pituitary adenomas and increases invasion in human pituitary adenoma cell line. Am J Pathol. 170:356–365. 2007. View Article : Google Scholar

21 

Pulukuri SM and Rao JS: Matrix metalloproteinase-1 promotes prostate tumor growth and metastasis. Int J Oncol. 32:757–765. 2008.PubMed/NCBI

22 

Xu D, McKee CM, Cao Y, et al: Matrix metalloproteinase-9 regulates tumor cell invasion through cleavage of protease nexin-1. Cancer Res. 70:6988–6998. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Kawaguchi-Ihara N, Murohashi I, Nara N and Tohda S: Promotion of the self-renewal capacity of human acute leukemia cells by Wnt3A. Anticancer Res. 28:2701–2704. 2008.PubMed/NCBI

24 

Hu J, Dong A, Fernandez-Ruiz V, et al: Blockade of Wnt signaling inhibits angiogenesis and tumor growth in hepatocellular carcinoma. Cancer Res. 69:6951–6959. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Kim D, Rath O, Kolch W and Cho KH: A hidden oncogenic positive feedback loop caused by crosstalk between Wnt and ERK pathways. Oncogene. 26:4571–4579. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Jeong AY, Lee MY, Lee SH, Park JH and Han HJ: PPARδ agonist-mediated ROS stimulates mouse embryonic stem cell proliferation through cooperation of p38 MAPK and Wnt/β-catenin. Cell Cycle. 8:611–619. 2009.

27 

Gazitt Y, Kolaparthi V, Moncada K, Thomas C and Freeman J: Targeted therapy of human osteosarcoma with 17AAG or rapamycin: Characterization of induced apoptosis and inhibition of mTOR and Akt/MAPK/Wnt pathways. Int J Oncol. 34:551–561. 2009.PubMed/NCBI

28 

Samara KD, Antoniou KM, Karagiannis K, et al: Expression profiles of Toll-like receptors in non-small cell lung cancer and idiopathic pulmonary fibrosis. Int J Oncol. 40:1397–1404. 2012.PubMed/NCBI

29 

Tanaka J, Sugimoto K, Shiraki K, et al: Functional cell surface expression of toll-like receptor 9 promotes cell proliferation and survival in human hepatocellular carcinomas. Int J Oncol. 37:805–814. 2010.PubMed/NCBI

30 

Takala H, Kauppila JH, Soini Y, et al: Toll-like receptor 9 is a novel biomarker for esophageal squamous cell dysplasia and squamous cell carcinoma progression. J Innate Immun. 3:631–638. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Qiu J, Shao S, Yang G, Shen Z and Zhang Y: Association of Toll like receptor 9 expression with lymph node metastasis in human breast cancer. Neoplasma. 58:251–255. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Lotze MT, Zeh HJ, Rubartelli A, et al: The grateful dead: damage-associated molecular pattern molecules and reduction/oxidation regulate immunity. Immunol Rev. 220:60–81. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Sato Y, Goto Y, Narita N and Hoon DS: Cancer cells expressing Toll-like receptors and the tumor microenvironment. Cancer Microenviron. 2(Suppl 1): 205–214. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Ikebe M, Kitaura Y, Nakamura M, et al: Lipopolysaccharide (LPS) increases the invasive ability of pancreatic cancer cells through the TLR4/MyD88 signaling pathway. J Surg Oncol. 100:725–731. 2009. View Article : Google Scholar : PubMed/NCBI

35 

Agha-Mohammadi S and Lotze MT: Immunomodulation of cancer: potential use of selectively replicating agents. J Clin Invest. 105:1173–1176. 2000. View Article : Google Scholar : PubMed/NCBI

36 

Tsai JP, Chen HW, Cheng ML, et al: Analysis of host versus tumor interaction in cancer patients: opposing role of transforming growth factor-β1 and interleukin-6 in the development of in situ tumor immunity. Immunobiology. 210:661–671. 2005.PubMed/NCBI

37 

Barbieri G, Rimini E and Costa MA: Effects of human leukocyte antigen (HLA)-DR engagement on melanoma cells. Int J Oncol. 38:1589–1595. 2011.PubMed/NCBI

38 

König R, Stertz S, Zhou Y, et al: Human host factors required for influenza virus replication. Nature. 463:813–817. 2010.PubMed/NCBI

39 

Chen MH, Lin KJ, Yang WL, et al: Gene expression-based chemical genomics identifies heat-shock protein 90 inhibitors as potential therapeutic drugs in cholangiocarcinoma. Cancer. 119:293–303. 2013. View Article : Google Scholar : PubMed/NCBI

40 

Wei G, Twomey D, Lamb J, et al: Gene expression-based chemical genomics identifies rapamycin as a modulator of MCL1 and glucocorticoid resistance. Cancer Cell. 10:331–342. 2006. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xue D, Lu M, Gao B, Qiao X and Zhang Y: Screening for transcription factors and their regulatory small molecules involved in regulating the functions of CL1-5 cancer cells under the effects of macrophage-conditioned medium. Oncol Rep 31: 1323-1333, 2014.
APA
Xue, D., Lu, M., Gao, B., Qiao, X., & Zhang, Y. (2014). Screening for transcription factors and their regulatory small molecules involved in regulating the functions of CL1-5 cancer cells under the effects of macrophage-conditioned medium. Oncology Reports, 31, 1323-1333. https://doi.org/10.3892/or.2013.2937
MLA
Xue, D., Lu, M., Gao, B., Qiao, X., Zhang, Y."Screening for transcription factors and their regulatory small molecules involved in regulating the functions of CL1-5 cancer cells under the effects of macrophage-conditioned medium". Oncology Reports 31.3 (2014): 1323-1333.
Chicago
Xue, D., Lu, M., Gao, B., Qiao, X., Zhang, Y."Screening for transcription factors and their regulatory small molecules involved in regulating the functions of CL1-5 cancer cells under the effects of macrophage-conditioned medium". Oncology Reports 31, no. 3 (2014): 1323-1333. https://doi.org/10.3892/or.2013.2937
Copy and paste a formatted citation
x
Spandidos Publications style
Xue D, Lu M, Gao B, Qiao X and Zhang Y: Screening for transcription factors and their regulatory small molecules involved in regulating the functions of CL1-5 cancer cells under the effects of macrophage-conditioned medium. Oncol Rep 31: 1323-1333, 2014.
APA
Xue, D., Lu, M., Gao, B., Qiao, X., & Zhang, Y. (2014). Screening for transcription factors and their regulatory small molecules involved in regulating the functions of CL1-5 cancer cells under the effects of macrophage-conditioned medium. Oncology Reports, 31, 1323-1333. https://doi.org/10.3892/or.2013.2937
MLA
Xue, D., Lu, M., Gao, B., Qiao, X., Zhang, Y."Screening for transcription factors and their regulatory small molecules involved in regulating the functions of CL1-5 cancer cells under the effects of macrophage-conditioned medium". Oncology Reports 31.3 (2014): 1323-1333.
Chicago
Xue, D., Lu, M., Gao, B., Qiao, X., Zhang, Y."Screening for transcription factors and their regulatory small molecules involved in regulating the functions of CL1-5 cancer cells under the effects of macrophage-conditioned medium". Oncology Reports 31, no. 3 (2014): 1323-1333. https://doi.org/10.3892/or.2013.2937
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team