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Article

Downregulation of microRNA-26a is associated with metastatic potential and the poor prognosis of osteosarcoma patients

  • Authors:
    • Qi-Chun Song
    • Zhi-Bin Shi
    • Yong-Tao Zhang
    • Le Ji
    • Kun‑Zheng Wang
    • Da-Peng Duan
    • Xiao-Qian Dang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Orthopedics, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China
  • Pages: 1263-1270
    |
    Published online on: January 21, 2014
       https://doi.org/10.3892/or.2014.2989
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Abstract

Accumulating evidence indicates that microRNAs are involved in multiple processes in cancer development and progression. microRNA-26a (miR-26a) has been identified as a tumor suppressor and its downregulation is associated with poor prognosis in several types of human cancer. However, the specific function of miR-26a in osteosarcoma remains unclear. In the present study, we found that the expression of miR-26a in osteosarcoma tissues and cell lines was much lower than that in the normal controls, respectively. In addition, downregulation of miR-26a more frequently occurred in osteosarcoma specimens with adverse clinical stage and with the presence of distant metastasis. Moreover, multivariate survival analyses demonstrated that loss of miR-26a is an independent prognostic factor for both disease-free and overall survival in osteosarcoma. In addition, restoration of miR-26a expression inhibited the invasion and migration in osteosarcoma cells, and miR-26a directly inhibited enhancer of zeste homolog 2 (EZH2) expression by targeting its 3'-UTR. Moreover, EZH2 was upregulated and inversely correlated with miR-26a expression in the osteosarcoma tissues. Thus, for the first time, we provide convincing evidence that downregulation of miR-26a is associated with tumor aggressiveness and tumor metastasis, and miR-26a inhibits cell migration and invasion by targeting the EZH2 gene in osteosarcoma. Thus, miR-26a is an independent prognostic marker for osteosarcoma patients.
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Copy and paste a formatted citation
Spandidos Publications style
Song Q, Shi Z, Zhang Y, Ji L, Wang KZ, Duan D and Dang X: Downregulation of microRNA-26a is associated with metastatic potential and the poor prognosis of osteosarcoma patients. Oncol Rep 31: 1263-1270, 2014.
APA
Song, Q., Shi, Z., Zhang, Y., Ji, L., Wang, K., Duan, D., & Dang, X. (2014). Downregulation of microRNA-26a is associated with metastatic potential and the poor prognosis of osteosarcoma patients. Oncology Reports, 31, 1263-1270. https://doi.org/10.3892/or.2014.2989
MLA
Song, Q., Shi, Z., Zhang, Y., Ji, L., Wang, K., Duan, D., Dang, X."Downregulation of microRNA-26a is associated with metastatic potential and the poor prognosis of osteosarcoma patients". Oncology Reports 31.3 (2014): 1263-1270.
Chicago
Song, Q., Shi, Z., Zhang, Y., Ji, L., Wang, K., Duan, D., Dang, X."Downregulation of microRNA-26a is associated with metastatic potential and the poor prognosis of osteosarcoma patients". Oncology Reports 31, no. 3 (2014): 1263-1270. https://doi.org/10.3892/or.2014.2989
Copy and paste a formatted citation
x
Spandidos Publications style
Song Q, Shi Z, Zhang Y, Ji L, Wang KZ, Duan D and Dang X: Downregulation of microRNA-26a is associated with metastatic potential and the poor prognosis of osteosarcoma patients. Oncol Rep 31: 1263-1270, 2014.
APA
Song, Q., Shi, Z., Zhang, Y., Ji, L., Wang, K., Duan, D., & Dang, X. (2014). Downregulation of microRNA-26a is associated with metastatic potential and the poor prognosis of osteosarcoma patients. Oncology Reports, 31, 1263-1270. https://doi.org/10.3892/or.2014.2989
MLA
Song, Q., Shi, Z., Zhang, Y., Ji, L., Wang, K., Duan, D., Dang, X."Downregulation of microRNA-26a is associated with metastatic potential and the poor prognosis of osteosarcoma patients". Oncology Reports 31.3 (2014): 1263-1270.
Chicago
Song, Q., Shi, Z., Zhang, Y., Ji, L., Wang, K., Duan, D., Dang, X."Downregulation of microRNA-26a is associated with metastatic potential and the poor prognosis of osteosarcoma patients". Oncology Reports 31, no. 3 (2014): 1263-1270. https://doi.org/10.3892/or.2014.2989
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