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Article

Thymoquinone induces G2/M arrest, inactivates PI3K/Akt and nuclear factor-κB pathways in human cholangiocarcinomas both in vitro and in vivo

  • Authors:
    • Dongsheng Xu
    • Yong Ma
    • Baolei Zhao
    • Shuai Li
    • Yu Zhang
    • Shangha Pan
    • Yaohua Wu
    • Jizhou Wang
    • Dawei Wang
    • Huayang Pan
    • Lianxin Liu
    • Hongchi Jiang
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Abdominal Ultrasound, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 2063-2070
    |
    Published online on: March 5, 2014
       https://doi.org/10.3892/or.2014.3059
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Abstract

Cholangiocarcinoma (CCA) is a notoriously lethal tumor mostly due to the de novo or acquired resistance to traditional chemotherapy besides gemcitabine and, therefore, an increasing need for effective strategies to prevent and treat the poor prognosis of this tumor is required. Thymoquinone (TQ), a hopeful natural product derived from black cumin (Nigella sativa) has shown considerable antineoplastic properties. Whether TQ exerts antitumor effects on CCA cells in vitro and in vivo remains unknown. Examinations of cell viability assay, detection of cell cycle and apoptosis, electrophoretic mobility shift assay (EMSA), western blotting and immunohistochemistry were used in the present study. We demonstrated that TQ inhibited the growth of human CCA cell lines (TFK-1 and HuCCT1) in a dose- and time-dependent manner. Firstly, our results provided evidence that TQ not only inhibits the proliferation of CCA cells, induces cell cycle arrest and prompts cell apoptotic effect in vitro, but it also exhibits inhibitory effects of tumor growth and angiogenesis in vivo. The responsible mechanism is at least partially due to TQ inhibiting the growth of CCA cell lines induced by downregulation of PI3K/Akt and NF-κB and regulated gene products, including p-AKT, p65, XIAP, Bcl-2, COX-2, VEGF. Taken together, these results provide strong evidence of our hypothesis that TQ alone presents a promising therapeutic regimen for the treatment of CCA cells with better efficiency.
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Copy and paste a formatted citation
Spandidos Publications style
Xu D, Ma Y, Zhao B, Li S, Zhang Y, Pan S, Wu Y, Wang J, Wang D, Pan H, Pan H, et al: Thymoquinone induces G2/M arrest, inactivates PI3K/Akt and nuclear factor-κB pathways in human cholangiocarcinomas both in vitro and in vivo. Oncol Rep 31: 2063-2070, 2014.
APA
Xu, D., Ma, Y., Zhao, B., Li, S., Zhang, Y., Pan, S. ... Jiang, H. (2014). Thymoquinone induces G2/M arrest, inactivates PI3K/Akt and nuclear factor-κB pathways in human cholangiocarcinomas both in vitro and in vivo. Oncology Reports, 31, 2063-2070. https://doi.org/10.3892/or.2014.3059
MLA
Xu, D., Ma, Y., Zhao, B., Li, S., Zhang, Y., Pan, S., Wu, Y., Wang, J., Wang, D., Pan, H., Liu, L., Jiang, H."Thymoquinone induces G2/M arrest, inactivates PI3K/Akt and nuclear factor-κB pathways in human cholangiocarcinomas both in vitro and in vivo". Oncology Reports 31.5 (2014): 2063-2070.
Chicago
Xu, D., Ma, Y., Zhao, B., Li, S., Zhang, Y., Pan, S., Wu, Y., Wang, J., Wang, D., Pan, H., Liu, L., Jiang, H."Thymoquinone induces G2/M arrest, inactivates PI3K/Akt and nuclear factor-κB pathways in human cholangiocarcinomas both in vitro and in vivo". Oncology Reports 31, no. 5 (2014): 2063-2070. https://doi.org/10.3892/or.2014.3059
Copy and paste a formatted citation
x
Spandidos Publications style
Xu D, Ma Y, Zhao B, Li S, Zhang Y, Pan S, Wu Y, Wang J, Wang D, Pan H, Pan H, et al: Thymoquinone induces G2/M arrest, inactivates PI3K/Akt and nuclear factor-κB pathways in human cholangiocarcinomas both in vitro and in vivo. Oncol Rep 31: 2063-2070, 2014.
APA
Xu, D., Ma, Y., Zhao, B., Li, S., Zhang, Y., Pan, S. ... Jiang, H. (2014). Thymoquinone induces G2/M arrest, inactivates PI3K/Akt and nuclear factor-κB pathways in human cholangiocarcinomas both in vitro and in vivo. Oncology Reports, 31, 2063-2070. https://doi.org/10.3892/or.2014.3059
MLA
Xu, D., Ma, Y., Zhao, B., Li, S., Zhang, Y., Pan, S., Wu, Y., Wang, J., Wang, D., Pan, H., Liu, L., Jiang, H."Thymoquinone induces G2/M arrest, inactivates PI3K/Akt and nuclear factor-κB pathways in human cholangiocarcinomas both in vitro and in vivo". Oncology Reports 31.5 (2014): 2063-2070.
Chicago
Xu, D., Ma, Y., Zhao, B., Li, S., Zhang, Y., Pan, S., Wu, Y., Wang, J., Wang, D., Pan, H., Liu, L., Jiang, H."Thymoquinone induces G2/M arrest, inactivates PI3K/Akt and nuclear factor-κB pathways in human cholangiocarcinomas both in vitro and in vivo". Oncology Reports 31, no. 5 (2014): 2063-2070. https://doi.org/10.3892/or.2014.3059
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