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Article

Evaluation of premature senescence and senescence biomarkers in carcinoma cells and xenograft mice exposed to single or fractionated irradiation

  • Authors:
    • Bong Cho Kim
    • Hee Jung Yoo
    • Hyung Chul Lee
    • Kyoung-Ah Kang
    • Seung Hee Jung
    • Hae-June Lee
    • Minyoung Lee
    • Seungwoo Park
    • Young-Hoon Ji
    • Yun-Sil Lee
    • Young-Gyu Ko
    • Jae-Seon Lee
  • View Affiliations / Copyright

    Affiliations: Research Center for Radio-senescence, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea, Division of Radiation Effects, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea, Research Center for Radiotherapy, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea, School of Pharmaceutical Sciences, Ewha Womans University, Seoul, Republic of Korea, Division of Life Sciences, Korea University, Seoul, Republic of Korea, Division of Biomedical Sciences, College of Medicine, Inha University, Incheon, Republic of Korea
  • Pages: 2229-2235
    |
    Published online on: March 7, 2014
       https://doi.org/10.3892/or.2014.3069
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Abstract

The purpose of the present study was to elucidate whether premature senescence contributes to the outcome of radiotherapy (RT) and to validate senescence biomarkers in vitro and in vivo. Cultured human cancer cell lines and xenografted mice were exposed to single (SR; 2, 6 or 12 Gy) or fractionated radiation (FR; 3 x 2 Gy or 6 x 2 Gy), and premature senescence was assessed using senescence-associated β-galactosidase (SA-β-Gal) activity, hypophosphorylation of pRb and p21 accumulation. A variety of senescence-associated biomarkers including cathepsin D (CD), the eukaryotic translation elongation factors eEF1A1, eEF1B2, decoy receptor 2 and Dec1 were further validated in vivo or in vitro. We demonstrated the beneficial tumor suppressive role of ionizing radiation (IR)-induced premature senescence in vitro and in vivo. FR inhibited tumor growth via induction of premature senescence as effectively as an equivalent SR dose (≥6 Gy). In addition, CD and eEF1 were valuable biomarkers of cellular senescence in either SR- or RF-exposed carcinoma cells or xenograft mice. Our results suggest that 2 Gy of a conventional RT regime could achieve a better clinical outcome if premature senescence could be increased through an improved understanding of its molecular action mechanism.
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Copy and paste a formatted citation
Spandidos Publications style
Kim BC, Yoo HJ, Lee HC, Kang K, Jung SH, Lee H, Lee M, Park S, Ji Y, Lee Y, Lee Y, et al: Evaluation of premature senescence and senescence biomarkers in carcinoma cells and xenograft mice exposed to single or fractionated irradiation. Oncol Rep 31: 2229-2235, 2014.
APA
Kim, B.C., Yoo, H.J., Lee, H.C., Kang, K., Jung, S.H., Lee, H. ... Lee, J. (2014). Evaluation of premature senescence and senescence biomarkers in carcinoma cells and xenograft mice exposed to single or fractionated irradiation. Oncology Reports, 31, 2229-2235. https://doi.org/10.3892/or.2014.3069
MLA
Kim, B. C., Yoo, H. J., Lee, H. C., Kang, K., Jung, S. H., Lee, H., Lee, M., Park, S., Ji, Y., Lee, Y., Ko, Y., Lee, J."Evaluation of premature senescence and senescence biomarkers in carcinoma cells and xenograft mice exposed to single or fractionated irradiation". Oncology Reports 31.5 (2014): 2229-2235.
Chicago
Kim, B. C., Yoo, H. J., Lee, H. C., Kang, K., Jung, S. H., Lee, H., Lee, M., Park, S., Ji, Y., Lee, Y., Ko, Y., Lee, J."Evaluation of premature senescence and senescence biomarkers in carcinoma cells and xenograft mice exposed to single or fractionated irradiation". Oncology Reports 31, no. 5 (2014): 2229-2235. https://doi.org/10.3892/or.2014.3069
Copy and paste a formatted citation
x
Spandidos Publications style
Kim BC, Yoo HJ, Lee HC, Kang K, Jung SH, Lee H, Lee M, Park S, Ji Y, Lee Y, Lee Y, et al: Evaluation of premature senescence and senescence biomarkers in carcinoma cells and xenograft mice exposed to single or fractionated irradiation. Oncol Rep 31: 2229-2235, 2014.
APA
Kim, B.C., Yoo, H.J., Lee, H.C., Kang, K., Jung, S.H., Lee, H. ... Lee, J. (2014). Evaluation of premature senescence and senescence biomarkers in carcinoma cells and xenograft mice exposed to single or fractionated irradiation. Oncology Reports, 31, 2229-2235. https://doi.org/10.3892/or.2014.3069
MLA
Kim, B. C., Yoo, H. J., Lee, H. C., Kang, K., Jung, S. H., Lee, H., Lee, M., Park, S., Ji, Y., Lee, Y., Ko, Y., Lee, J."Evaluation of premature senescence and senescence biomarkers in carcinoma cells and xenograft mice exposed to single or fractionated irradiation". Oncology Reports 31.5 (2014): 2229-2235.
Chicago
Kim, B. C., Yoo, H. J., Lee, H. C., Kang, K., Jung, S. H., Lee, H., Lee, M., Park, S., Ji, Y., Lee, Y., Ko, Y., Lee, J."Evaluation of premature senescence and senescence biomarkers in carcinoma cells and xenograft mice exposed to single or fractionated irradiation". Oncology Reports 31, no. 5 (2014): 2229-2235. https://doi.org/10.3892/or.2014.3069
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