Arecoline inhibits epithelial cell viability by upregulating the apoptosis pathway: Implication for oral submucous fibrosis

Li,Ming; Gao,Feng; Zhou,Zhong-Su; Zhang,Hui-Ming; Zhang,Rui; Wu,Ying-Fang; Bai,Ming-Hai; Li,Ji-Jia; Lin,Shi-Rong; Peng,Jie-Ying

doi:10.3892/or.2014.3091

Arecoline inhibits epithelial cell viability by upregulating the apoptosis pathway: Implication for oral submucous fibrosis

Authors:
- Ming Li
- Feng Gao
- Zhong-Su Zhou
- Hui-Ming Zhang
- Rui Zhang
- Ying-Fang Wu
- Ming-Hai Bai
- Ji-Jia Li
- Shi-Rong Lin
- Jie-Ying Peng
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Affiliations: Dental Medical Center, Xiangya Hospital of Central South University, Changsha, Hunan, P.R. China, Powder Metallurgy Research Institute of Central South University, Changsha, Hunan, P.R. China, Changsha Stomatological Hospital, Changsha, Hunan, P.R. China, Taiwan Taipei Dental Sciences, Taipei, Taiwan, R.O.C.
Published online on: March 14, 2014 https://doi.org/10.3892/or.2014.3091
Pages: 2422-2428

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Abstract

Oral submucous fibrosis (OSF) is a chronic inflammatory disease characterized by the accumulation of excess collagen, and areca nut chewing has been proposed as a significant etiological factor for disease manifestation. However, the underlying molecular mechanisms regarding areca nut chewing-induced OSF are only partially understood. Herein, we reported that arecoline markedly induced morphologic change in HaCaT epithelial cells, but had no obvious effect on Hel fibroblast cells. MTS assay revealed that arecoline significantly suppressed HaCaT cell viability. Moreover, flow cytometric analysis indicated that arecoline substantially promoted HaCaT cell, but not Hel cell apoptosis in a dose-dependent manner. Furthermore, arecoline-induced HaCaT cell apoptosis was found to be associated with increased expression and activation of cleaved-Bid, cleaved-PARA and cleaved-caspase-3. Collectively, our results suggest that HaCaT epithelial cells are more sensitive than Hel fibroblast cells to arecoline-induced cytotoxicity, which may be involved in the pathogenesis of OSF.

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