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Article

Low expression of GABARAPL1 is associated with a poor outcome for patients with hepatocellular carcinoma

  • Authors:
    • Chao Liu
    • Yan Xia
    • Wei Jiang
    • Yinkun Liu
    • Long Yu
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China, Liver Cancer Institute, Fudan University, Shanghai 200032, P.R. China
  • Pages: 2043-2048
    |
    Published online on: March 19, 2014
       https://doi.org/10.3892/or.2014.3096
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Abstract

Autophagy is an evolutionarily conserved cellular process that degrades cytoplasmic materials through the lysosomal pathway. The deregulation of autophagy is associated with several diseases, particularly cancer. Hepatocellular carcinoma (HCC) is one of the most aggressive cancers with a poor prognosis. The expression of autophagy-related genes in HCC and their relationships with HCC are largely unknown. In the present study, we analyzed the expression of autophagy-related genes based on the Oncomine database and quantitative PCR of HCC and adjacent liver tissues. We found that the mRNA and protein expression of GABARAPL1 was significantly decreased in HCC tissues compared with their adjacent liver tissues. In HCC cancer cell lines, overexpression of GABARAPL1 inhibited cell growth, while knockdown of GABARAPL1 expression via siRNA promoted cell growth. In addition, we found a significant correlation of low GABARAPL1 expression with poor differentiation of HCC cells (P=0.018), and with the absence of tumor capsules (P=0.047). Kaplan-Meier survival analysis showed a significant association between low GABARAPL1 expression and poor prognosis of HCC patients (P=0.0094). Our data showed for the first time that GABARAPL1 expression is associated with poor prognosis of HCC patients.
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1 

Yang JD and Roberts LR: Hepatocellular carcinoma: a global view. Nat Rev Gastroenterol Hepatol. 7:448–458. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar

3 

Klionsky DJ and Emr SD: Autophagy as a regulated pathway of cellular degradation. Science. 290:1717–1721. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Yang Z and Klionsky DJ: Eaten alive: a history of macroautophagy. Nat Cell Biol. 12:814–822. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Shintani T and Klionsky DJ: Autophagy in health and disease: a double-edged sword. Science. 306:990–995. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Li Y, Zhang J, Chen X, et al: Molecular machinery of autophagy and its implication in cancer. Am J Med Sci. 343:155–161. 2011. View Article : Google Scholar

7 

Rautou PE, Mansouri A, Lebrec D, Durand F, Valla D and Moreau R: Autophagy in liver diseases. J Hepatol. 53:1123–1134. 2010. View Article : Google Scholar

8 

Pandey S and Chandravati: Autophagy in cervical cancer: an emerging therapeutic target. Asian Pac J Cancer Prev. 13:4867–4871. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Kaza N, Kohli L and Roth KA: Autophagy in brain tumors: a new target for therapeutic intervention. Brain Pathol. 22:89–98. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Cook KL, Shajahan AN and Clarke R: Autophagy and endocrine resistance in breast cancer. Expert Rev Anticancer Ther. 11:1283–1294. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Yue Z, Jin S, Yang C, Levine AJ and Heintz N: Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor. Proc Natl Acad Sci USA. 100:15077–15082. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Liang XH, Jackson S, Seaman M, et al: Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature. 402:672–676. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Qu X, Yu J, Bhagat G, et al: Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene. J Clin Invest. 112:1809–1820. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Takahashi Y, Coppola D, Matsushita N, et al: Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis. Nat Cell Biol. 9:1142–1151. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Ding ZB, Shi YH, Zhou J, et al: Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma. Cancer Res. 68:9167–9175. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Pellerin I, Vuillermoz C, Jouvenot M, Ordener C, Royez M and Adessi GL: Identification and characterization of an early estrogen-regulated RNA in cultured guinea-pig endometrial cells. Mol Cell Endocrinol. 90:R17–R21. 1993. View Article : Google Scholar : PubMed/NCBI

17 

Jouvenot M, Pellerin I, Alkhalaf M, Marechal G, Royez M and Adessi GL: Effects of 17 beta-estradiol and growth factors on c-fos gene expression in endometrial epithelial cells in primary culture. Mol Cell Endocrinol. 72:149–157. 1990. View Article : Google Scholar : PubMed/NCBI

18 

Chakrama FZ, Seguin-Py S, Le Grand JN, et al: GABARAPL1 (GEC1) associates with autophagic vesicles. Autophagy. 6:495–505. 2010. View Article : Google Scholar

19 

Pankiv S, Clausen TH, Lamark T, et al: p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy. J Biol Chem. 282:24131–24145. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Berthier A, Seguin S, Sasco AJ, et al: High expression of gabarapl1 is associated with a better outcome for patients with lymph node-positive breast cancer. Br J Cancer. 102:1024–1031. 2010.

21 

Wittekind C: Pitfalls in the classification of liver tumors. Pathologe. 27:289–293. 2006.(In German).

22 

Rhodes DR, Yu J, Shanker K, et al: ONCOMINE: a cancer microarray database and integrated data-mining platform. Neoplasia. 6:1–6. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Chen X, Cheung ST, So S, et al: Gene expression patterns in human liver cancers. Mol Biol Cell. 13:1929–1939. 2002. View Article : Google Scholar PubMed/NCBI

24 

Roessler S, Jia HL, Budhu A, et al: A unique metastasis gene signature enables prediction of tumor relapse in early-stage hepatocellular carcinoma patients. Cancer Res. 70:10202–10212. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Wurmbach E, Chen YB, Khitrov G, et al: Genome-wide molecular profiles of HCV-induced dysplasia and hepatocellular carcinoma. Hepatology. 45:938–947. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Mas VR, Maluf DG, Archer KJ, et al: Genes involved in viral carcinogenesis and tumor initiation in hepatitis C virus-induced hepatocellular carcinoma. Mol Med. 15:85–94. 2009.PubMed/NCBI

27 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001.

28 

Wu Y, Zuo J, Ji G, et al: Proapoptotic function of integrin β3 in human hepatocellular carcinoma cells. Clin Cancer Res. 15:60–69. 2009.

29 

Rhodes DR, Kalyana-Sundaram S, Mahavisno V, et al: Oncomine 3.0: genes, pathways, and networks in a collection of 18,000 cancer gene expression profiles. Neoplasia. 9:166–180. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Inoue D, Suzuki T, Mitsuishi Y, et al: Accumulation of p62/SQSTM1 is associated with poor prognosis in patients with lung adenocarcinoma. Cancer Sci. 103:760–766. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Klebig C, Seitz S, Arnold W, et al: Characterization of γ-aminobutyric acid type A receptor-associated protein, a novel tumor suppressor, showing reduced expression in breast cancer. Cancer Res. 65:394–400. 2005.

32 

Liu GH, Zhong Q, Ye YL, et al: Expression of beclin 1 in bladder cancer and its clinical significance. Int J Biol Markers. 28:56–62. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Alemu EA, Lamark T, Torgersen KM, et al: ATG8 family proteins act as scaffolds for assembly of the ULK complex: sequence requirements for LC3-interacting region (LIR) motifs. J Biol Chem. 287:39275–39290. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Weidberg H, Shvets E, Shpilka T, Shimron F, Shinder V and Elazar Z: LC3 and GATE-16/GABARAP subfamilies are both essential yet act differently in autophagosome biogenesis. EMBO J. 29:1792–1802. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Roberts SS, Mori M, Pattee P, et al: GABAergic system gene expression predicts clinical outcome in patients with neuroblastoma. J Clin Oncol. 22:4127–4134. 2004. View Article : Google Scholar : PubMed/NCBI

36 

Zhang Y, Wang F, Han L, et al: GABARAPL1 negatively regulates Wnt/β-catenin signaling by mediating Dvl2 degradation through the autophagy pathway. Cell Physiol Biochem. 27:503–512. 2011.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu C, Xia Y, Jiang W, Liu Y and Yu L: Low expression of GABARAPL1 is associated with a poor outcome for patients with hepatocellular carcinoma. Oncol Rep 31: 2043-2048, 2014.
APA
Liu, C., Xia, Y., Jiang, W., Liu, Y., & Yu, L. (2014). Low expression of GABARAPL1 is associated with a poor outcome for patients with hepatocellular carcinoma. Oncology Reports, 31, 2043-2048. https://doi.org/10.3892/or.2014.3096
MLA
Liu, C., Xia, Y., Jiang, W., Liu, Y., Yu, L."Low expression of GABARAPL1 is associated with a poor outcome for patients with hepatocellular carcinoma". Oncology Reports 31.5 (2014): 2043-2048.
Chicago
Liu, C., Xia, Y., Jiang, W., Liu, Y., Yu, L."Low expression of GABARAPL1 is associated with a poor outcome for patients with hepatocellular carcinoma". Oncology Reports 31, no. 5 (2014): 2043-2048. https://doi.org/10.3892/or.2014.3096
Copy and paste a formatted citation
x
Spandidos Publications style
Liu C, Xia Y, Jiang W, Liu Y and Yu L: Low expression of GABARAPL1 is associated with a poor outcome for patients with hepatocellular carcinoma. Oncol Rep 31: 2043-2048, 2014.
APA
Liu, C., Xia, Y., Jiang, W., Liu, Y., & Yu, L. (2014). Low expression of GABARAPL1 is associated with a poor outcome for patients with hepatocellular carcinoma. Oncology Reports, 31, 2043-2048. https://doi.org/10.3892/or.2014.3096
MLA
Liu, C., Xia, Y., Jiang, W., Liu, Y., Yu, L."Low expression of GABARAPL1 is associated with a poor outcome for patients with hepatocellular carcinoma". Oncology Reports 31.5 (2014): 2043-2048.
Chicago
Liu, C., Xia, Y., Jiang, W., Liu, Y., Yu, L."Low expression of GABARAPL1 is associated with a poor outcome for patients with hepatocellular carcinoma". Oncology Reports 31, no. 5 (2014): 2043-2048. https://doi.org/10.3892/or.2014.3096
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