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Article

Interaction of ribosomal protein L22 with casein kinase 2α: A novel mechanism for understanding the biology of non-small cell lung cancer

  • Authors:
    • Mingxia Yang
    • Haibo Sun
    • Ji He
    • Hong Wang
    • Xiaowei Yu
    • Lei Ma
    • Changliang Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The Affiliated Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China, Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, State Key Laboratory of Monitoring and Detection for Medical Vectors, Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen, Fujian 361012, P.R. China, Department of Respiratory Medicine, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 139-144
    |
    Published online on: May 15, 2014
       https://doi.org/10.3892/or.2014.3187
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Abstract

Dysfunction of ribosomal proteins (RPs) may play an important role in molecular tumorigenesis, such as lung cancer, acting in extraribosomal functions. Many protein-protein interaction studies and genetic screens have confirmed the extraribosomal capacity of RPs. As reported, ribosomal protein L22 (RPL22) dysfunction could increase cancer risk. In the present study, we examined RPL22-protein complexes in lung cancer cells. Tandem affinity purification (TAP) was used to screen the RPL22-protein complexes, and GST pull-down experiments and confocal microscopy were used to assess the protein-protein interaction. The experiment of kinase assay was used to study the function of the RPL22-protein complexes. The results showed that several differentially expressed proteins were isolated and identified by LC-MS/MS, which revealed that one of the protein complexes included casein kinase 2α (CK2α). RPL22 and CK2α interact in vitro. RPL22 also inhibited CK2α substrate phosphorylation in vitro. This is the first report of the RPL22-CK2α relationship in lung cancer. Dysregulated CK2 may impact cell proliferation and apoptosis, key features of cancer cell biology. Our results indicate that RPL22 may be a candidate anticancer agent due to its CK2α-binding and -inhibitory functions in human lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Yang M, Sun H, He J, Wang H, Yu X, Ma L and Zhu C: Interaction of ribosomal protein L22 with casein kinase 2α: A novel mechanism for understanding the biology of non-small cell lung cancer. Oncol Rep 32: 139-144, 2014.
APA
Yang, M., Sun, H., He, J., Wang, H., Yu, X., Ma, L., & Zhu, C. (2014). Interaction of ribosomal protein L22 with casein kinase 2α: A novel mechanism for understanding the biology of non-small cell lung cancer. Oncology Reports, 32, 139-144. https://doi.org/10.3892/or.2014.3187
MLA
Yang, M., Sun, H., He, J., Wang, H., Yu, X., Ma, L., Zhu, C."Interaction of ribosomal protein L22 with casein kinase 2α: A novel mechanism for understanding the biology of non-small cell lung cancer". Oncology Reports 32.1 (2014): 139-144.
Chicago
Yang, M., Sun, H., He, J., Wang, H., Yu, X., Ma, L., Zhu, C."Interaction of ribosomal protein L22 with casein kinase 2α: A novel mechanism for understanding the biology of non-small cell lung cancer". Oncology Reports 32, no. 1 (2014): 139-144. https://doi.org/10.3892/or.2014.3187
Copy and paste a formatted citation
x
Spandidos Publications style
Yang M, Sun H, He J, Wang H, Yu X, Ma L and Zhu C: Interaction of ribosomal protein L22 with casein kinase 2α: A novel mechanism for understanding the biology of non-small cell lung cancer. Oncol Rep 32: 139-144, 2014.
APA
Yang, M., Sun, H., He, J., Wang, H., Yu, X., Ma, L., & Zhu, C. (2014). Interaction of ribosomal protein L22 with casein kinase 2α: A novel mechanism for understanding the biology of non-small cell lung cancer. Oncology Reports, 32, 139-144. https://doi.org/10.3892/or.2014.3187
MLA
Yang, M., Sun, H., He, J., Wang, H., Yu, X., Ma, L., Zhu, C."Interaction of ribosomal protein L22 with casein kinase 2α: A novel mechanism for understanding the biology of non-small cell lung cancer". Oncology Reports 32.1 (2014): 139-144.
Chicago
Yang, M., Sun, H., He, J., Wang, H., Yu, X., Ma, L., Zhu, C."Interaction of ribosomal protein L22 with casein kinase 2α: A novel mechanism for understanding the biology of non-small cell lung cancer". Oncology Reports 32, no. 1 (2014): 139-144. https://doi.org/10.3892/or.2014.3187
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