PinX1 without the G-patch motif suppresses proliferation, induces senescence, but does not inhibit telomerase activity in colorectal cancer SW480 cells

Zhang,Rui; Zhao,Jian; Wang,Xu; Wang,Li-Li; Xu,Jian; Song,Chun

doi:10.3892/or.2014.3199

July-2014 Volume 32 Issue 1

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July-2014 Volume 32 Issue 1

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Article

PinX1 without the G-patch motif suppresses proliferation, induces senescence, but does not inhibit telomerase activity in colorectal cancer SW480 cells

Authors:
- Rui Zhang
- Jian Zhao
- Xu Wang
- Li-Li Wang
- Jian Xu
- Chun Song
View Affiliations / Copyright

Affiliations: Department of Colorectal Surgery, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110042, P.R. China
Pages: 286-292
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Published online on: May 20, 2014

https://doi.org/10.3892/or.2014.3199
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Abstract

Evidence suggests that Pin2/TRF1-interacting protein X1 (PinX1) inhibits telomerase activity in many types of cancer cells. G-patch is a motif in the PinX1 protein; however, the function of G-patch in colorectal cancer cells has not been definitively elucidated. The present study investigated the antitumor activities of different PinX1 fragments in vitro, and explored the molecular mechanisms responsible for these effects. SW480 cells were transfected with pEGFP-A1-PinX1 1-328 (intact) or pEGFP-A1-PinX1 69-328 (truncated). Flow cytometry was used to observe apoptosis and the cell cycle of SW480 cells transfected with intact PinX1 or truncated PinX1. The apoptosis-related proteins, caspase 3, 8 and 9, were detected by western blotting. Our results indicate that both intact and truncated PinX1 induced apoptosis, G1 arrest, and cellular senescence. However, truncated PinX1 showed no effects on telomerase activity. Why PinX1 without G-patch has similar antitumor activities as intact PinX1 remains unclear. The mechanisms of G-patch require elucidation in subsequent studies. Finally, we detected the protein and mRNA levels of PinX1 and caspase 3, 8 and 9 in colorectal cancer specimens and confirmed that levels of PinX1 and caspase 3, 8 and 9 expression were closely linked to the poor prognosis of colorectal cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

PinX1 without the G-patch motif suppresses proliferation, induces senescence, but does not inhibit telomerase activity in colorectal cancer SW480 cells

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