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Article

The dineolignan from Saururus chinensis, manassantin B, inhibits tumor-induced angiogenesis via downregulation of matrix metalloproteinases 9 in human endothelial cells

  • Authors:
    • Zhaojie Liu
    • Hong Lu
    • Rong  Liu
    • Bin  Chen
    • Shan  Wang
    • Junchao  Ma
    • Jianjiang  Fu
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China, Network and Educational Technology Center, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China, State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, P.R. China
  • Pages: 659-667
    |
    Published online on: June 11, 2014
       https://doi.org/10.3892/or.2014.3244
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Abstract

Manassantin B (MB) is a neolignan isolated from Saururus chinensis that exhibits a range of activities, inclu­ding anti-inflammatory, antiseptic and antitumor activity. MB was recently found to affect cell adhesion and expression of several adhesion molecules. Based on the important roles of these adhesion molecules in angiogenesis, we evaluated a possible role for MB in tumor-induced angiogenesis in endothelial cells (ECs). In the present study, we found that MB blocked tumor-induced tube formation of ECs and significantly inhibited the invasion of ECs through the reconstituted basement membrane. MB suppressed the activity of matrix metalloproteinases (MMPs) and downregulated the expression of matrix metalloproteinases 9. Western blotting showed reduction of RUNX2 activation by MB. RUNX2 transcription factor assay and chromatin immunoprecipitation assay showed that the interaction between RUNX2 and target sequences in the matrix metalloproteinases 9 promoters was inhibited by MB. Our findings suggested that the inhibitory effects of MB on tumor-induced angiogenesis were caused by matrix metalloproteinases 9 inhibition, which was associated with the downregulation of RUNX2 transcriptional activity.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Z, Lu H, Liu R, Chen B, Wang S, Ma J and Fu J: The dineolignan from Saururus chinensis, manassantin B, inhibits tumor-induced angiogenesis via downregulation of matrix metalloproteinases 9 in human endothelial cells. Oncol Rep 32: 659-667, 2014.
APA
Liu, Z., Lu, H., Liu, R., Chen, B., Wang, S., Ma, J., & Fu, J. (2014). The dineolignan from Saururus chinensis, manassantin B, inhibits tumor-induced angiogenesis via downregulation of matrix metalloproteinases 9 in human endothelial cells. Oncology Reports, 32, 659-667. https://doi.org/10.3892/or.2014.3244
MLA
Liu, Z., Lu, H., Liu, R., Chen, B., Wang, S., Ma, J., Fu, J."The dineolignan from Saururus chinensis, manassantin B, inhibits tumor-induced angiogenesis via downregulation of matrix metalloproteinases 9 in human endothelial cells". Oncology Reports 32.2 (2014): 659-667.
Chicago
Liu, Z., Lu, H., Liu, R., Chen, B., Wang, S., Ma, J., Fu, J."The dineolignan from Saururus chinensis, manassantin B, inhibits tumor-induced angiogenesis via downregulation of matrix metalloproteinases 9 in human endothelial cells". Oncology Reports 32, no. 2 (2014): 659-667. https://doi.org/10.3892/or.2014.3244
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Lu H, Liu R, Chen B, Wang S, Ma J and Fu J: The dineolignan from Saururus chinensis, manassantin B, inhibits tumor-induced angiogenesis via downregulation of matrix metalloproteinases 9 in human endothelial cells. Oncol Rep 32: 659-667, 2014.
APA
Liu, Z., Lu, H., Liu, R., Chen, B., Wang, S., Ma, J., & Fu, J. (2014). The dineolignan from Saururus chinensis, manassantin B, inhibits tumor-induced angiogenesis via downregulation of matrix metalloproteinases 9 in human endothelial cells. Oncology Reports, 32, 659-667. https://doi.org/10.3892/or.2014.3244
MLA
Liu, Z., Lu, H., Liu, R., Chen, B., Wang, S., Ma, J., Fu, J."The dineolignan from Saururus chinensis, manassantin B, inhibits tumor-induced angiogenesis via downregulation of matrix metalloproteinases 9 in human endothelial cells". Oncology Reports 32.2 (2014): 659-667.
Chicago
Liu, Z., Lu, H., Liu, R., Chen, B., Wang, S., Ma, J., Fu, J."The dineolignan from Saururus chinensis, manassantin B, inhibits tumor-induced angiogenesis via downregulation of matrix metalloproteinases 9 in human endothelial cells". Oncology Reports 32, no. 2 (2014): 659-667. https://doi.org/10.3892/or.2014.3244
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