A single nucleotide polymorphism in fibronectin 1 determines tumor shape in colorectal cancer

Kida,Hiroyuki; Takano,Yuki; Yamamoto,Ken; Mori,Masaki; Yanaga,Katsuhiko; Tanaka,Jun-Ichi; Kudo,Shin-Ei; Mimori,Koshi

doi:10.3892/or.2014.3251

August-2014 Volume 32 Issue 2

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

August-2014 Volume 32 Issue 2

Full Size Image

Article

A single nucleotide polymorphism in fibronectin 1 determines tumor shape in colorectal cancer

Authors:
- Hiroyuki Kida
- Yuki Takano
- Ken Yamamoto
- Masaki Mori
- Katsuhiko Yanaga
- Jun-Ichi Tanaka
- Shin-Ei Kudo
- Koshi Mimori
View Affiliations / Copyright

Affiliations: Digestive Disease Center, Northern Yokohama Hospital, Showa University, Yokohama, Japan, Department of Surgery, Kyushu University Beppu Hospital, Beppu, Japan, Department of Molecular and Cellular Biology, Kyushu University, Fukuoka, Japan, Department of Gastroenterology, Osaka University, Suita, Japan, Department of Surgery, Jikei University School of Medicine, Tokyo, Japan
Pages: 548-552
|
Published online on: June 12, 2014

https://doi.org/10.3892/or.2014.3251
Expand metrics +

Abstract

Depressed and flat surface lesions are not easy to identify with routine colonoscopies during screening for colorectal cancer (CRC). Identifying clinically relevant genes that influence tumor shape could be useful when screening for the presence of depressed lesions. Total RNA was extracted from tumor cells collected by laser microdissection from the primary lesions of 146 CRC cases. Microarray analysis was performed to identify genes that were differentially expressed between depressed and elevated tumors. Single nucleotide polymorphism (SNP) analysis of genomic DNA from the peripheral blood of 67 CRC patients was then used to associate polymorphisms with the occurrence of depressed tumors. Microarray analysis revealed significantly higher expression of the fibronectin 1 (FN1) gene in 129 depressed-type tumors and lesions compared to 17 elevated-type tumors. FN1-abundant CRC tumors were large with a significantly higher incidence of lymphatic permeation. SNP analysis indicated that 44 tumors with a GG genotype at SNP rs6707530 showed significantly higher FN1 expression than did 23 tumors with GT/TT genotypes (p<0.05). The product of the FN1 gene (located at 2q34) is involved in cell adhesion, migration and metastasis in mesenchymal tumors. Abundant expression of FN1 may allow cancer cells to invade deeper layers, which would eventually define tumor shape. Identification of this SNP in blood samples may facilitate disease diagnosis and allow prediction of the presence of depressed tumors in the colorectal epithelium before a colon fiberscope examination.

View Figures

View References

1	Jass JR, Baker K, Zlobec I, et al: Advanced colorectal polyps with the molecular and morphological features of serrated polyps and adenomas: concept of a ‘fusion’ pathway to colorectal cancer. Histopathology. 49:121–131. 2006.PubMed/NCBI
2	Jass JR, Biden KG, Cummings MC, et al: Characterisation of a subtype of colorectal cancer combining features of the suppressor and mild mutator pathways. J Clin Pathol. 52:455–460. 1999. View Article : Google Scholar : PubMed/NCBI
3	Snover DC, Jass JR, Fenoglio-Preiser C and Batts KP: Serrated polyps of the large intestine: a morphologic and molecular review of an evolving concept. Am J Clin Pathol. 124:380–391. 2005. View Article : Google Scholar : PubMed/NCBI
4	Vogelstein B, Fearon ER, Hamilton SR, et al: Genetic alterations during colorectal-tumor development. N Engl J Med. 319:525–532. 1988. View Article : Google Scholar : PubMed/NCBI
5	Kudo S, Lambert R, Allen JI, et al: Nonpolypoid neoplastic lesions of the colorectal mucosa. Gastrointest Endosc. 68(Suppl 4): S3–S47. 2008. View Article : Google Scholar : PubMed/NCBI
6	Kudo S, Kashida H, Tamura S and Nakajima T: The problem of ‘flat’ colonic adenoma. Gastrointest Endosc Clin N Am. 7:87–98. 1997.
7	Kudo S, Tamura S, Hirota S, et al: The problem of de novo colorectal carcinoma. Eur J Cancer. 31A:1118–1120. 1995. View Article : Google Scholar : PubMed/NCBI
8	The Paris endoscopic classification of superficial neoplastic lesions: esophagus, stomach, and colon: November 30 to December 1, 2002. Gastrointest Endosc. 58(Suppl 6): S3–S43. 2003. View Article : Google Scholar : PubMed/NCBI
9	Update on the Paris classification of superficial neoplastic lesions in the digestive tract. Endoscopy. 37:570–578. 2005. View Article : Google Scholar : PubMed/NCBI
10	Goossens K, Van Soom A, Van Zeveren A, Favoreel H and Peelman LJ: Quantification of fibronectin 1 (FN1) splice variants, including two novel ones, and analysis of integrins as candidate FN1 receptors in bovine preimplantation embryos. BMC Dev Biol. 9:12009. View Article : Google Scholar : PubMed/NCBI
11	Sato Y, Yoshizato T, Shiraishi Y, et al: Integrated molecular analysis of clear-cell renal cell carcinoma. Nat Genet. 45:860–867. 2013. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A single nucleotide polymorphism in fibronectin 1 determines tumor shape in colorectal cancer

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles