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September-2014 Volume 32 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31

  • Authors:
    • Chaoyin Jiang
    • Hua Chen
    • Lei Shao
    • Qiaojie Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
  • Pages: 1249-1256
    |
    Published online on: June 20, 2014
       https://doi.org/10.3892/or.2014.3274
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Abstract

MicroRNA-1 (miR-1) has been shown to function as a critical gene regulator in multiple types of cancers. However, the role of miR-1 in osteosarcoma has not been totally clarified. In the present study, we investigated the effects of miR-1 on osteosarcoma and the underlying mechanism. We found that miR-1 was downregulated in osteosarcoma tissues and osteosarcoma cell lines. Restoration of miR-1 significantly suppressed osteosarcoma cell proliferation by inhibiting cell cycle progression. Mediator complex subunit 1 (Med1) and 31 (Med31) were validated as targets of miR-1 in osteosarcoma by luciferase reporter assay. Downregulation of Med1 and Med31 suppressed the proliferation of osteosarcoma cells, and overexpression of Med1 and Med31 abrogated the effects of miR-1 on cell proliferation. Furthermore, both miR-1 and knockdown of Med1 or Med31 reduced the expression of met proto-oncogene (MET) and blocked the downstream signaling of MET responding to hepatocyte growth factor (HGF). Taken together, the findings of this study suggest that Med1 and Med31 serve as potential gene therapeutic targets in osteosarcoma and miR-1 may prove to be a promising agent.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang C, Chen H, Shao L and Wang Q: MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31. Oncol Rep 32: 1249-1256, 2014.
APA
Jiang, C., Chen, H., Shao, L., & Wang, Q. (2014). MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31. Oncology Reports, 32, 1249-1256. https://doi.org/10.3892/or.2014.3274
MLA
Jiang, C., Chen, H., Shao, L., Wang, Q."MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31". Oncology Reports 32.3 (2014): 1249-1256.
Chicago
Jiang, C., Chen, H., Shao, L., Wang, Q."MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31". Oncology Reports 32, no. 3 (2014): 1249-1256. https://doi.org/10.3892/or.2014.3274
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang C, Chen H, Shao L and Wang Q: MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31. Oncol Rep 32: 1249-1256, 2014.
APA
Jiang, C., Chen, H., Shao, L., & Wang, Q. (2014). MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31. Oncology Reports, 32, 1249-1256. https://doi.org/10.3892/or.2014.3274
MLA
Jiang, C., Chen, H., Shao, L., Wang, Q."MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31". Oncology Reports 32.3 (2014): 1249-1256.
Chicago
Jiang, C., Chen, H., Shao, L., Wang, Q."MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31". Oncology Reports 32, no. 3 (2014): 1249-1256. https://doi.org/10.3892/or.2014.3274
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