MicroRNA-451 regulates activating transcription factor 2 expression and inhibits liver cancer cell migration

  • Authors:
    • Guixiang Lv
    • Zheng Hu
    • Yi Tie
    • Jingli Du
    • Hanjiang Fu
    • Xu Gao
    • Xiaofei Zheng
  • View Affiliations

  • Published online on: June 27, 2014     https://doi.org/10.3892/or.2014.3296
  • Pages: 1021-1028
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Abstract

Accumulating evidence suggests that microRNAs (miRNAs) can function as oncogenes or as tumor suppressor genes depending on the tissue type or target. Therefore, clarification of the specific roles of miRNAs is vital for the diagnosis and treatment of cancer. In the present study, miR-451 was found to be downregulated in hepatocellular carcinoma (HCC) tissues when compared to that in adjacent tissues. Functional analysis showed that, in vitro, miR-451 inhibited the migration of hepatoma cell lines HepG2 and SK-Hep-1. Further investigation of the molecular mechanisms identified activating transcription factor 2 (ATF2) as a target of miR-451. miR-451 inhibited ATF2 expression by binding to the 3'UTR. An in vivo assay revealed a significant negative correlation between miR-451 and ATF2 in liver cancer tissues. According to previous findings reported in the literature, the opposing functions of ATF2 are related to its subcellular localization. In the nucleus, ATF2 displays oncogenic activities in melanoma. In the present study, ATF2 exhibited a higher expression level in the nucleus in tumoral tissues of HCC as detected by immunohistochemistry. In conclusion, in this study, we identified a potential target of miR-451, ATF2, and revealed a novel role of miR-451 in the inhibition of the migratory ability of hepatoma cell lines.
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September-2014
Volume 32 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lv G, Hu Z, Tie Y, Du J, Fu H, Gao X and Zheng X: MicroRNA-451 regulates activating transcription factor 2 expression and inhibits liver cancer cell migration. Oncol Rep 32: 1021-1028, 2014
APA
Lv, G., Hu, Z., Tie, Y., Du, J., Fu, H., Gao, X., & Zheng, X. (2014). MicroRNA-451 regulates activating transcription factor 2 expression and inhibits liver cancer cell migration. Oncology Reports, 32, 1021-1028. https://doi.org/10.3892/or.2014.3296
MLA
Lv, G., Hu, Z., Tie, Y., Du, J., Fu, H., Gao, X., Zheng, X."MicroRNA-451 regulates activating transcription factor 2 expression and inhibits liver cancer cell migration". Oncology Reports 32.3 (2014): 1021-1028.
Chicago
Lv, G., Hu, Z., Tie, Y., Du, J., Fu, H., Gao, X., Zheng, X."MicroRNA-451 regulates activating transcription factor 2 expression and inhibits liver cancer cell migration". Oncology Reports 32, no. 3 (2014): 1021-1028. https://doi.org/10.3892/or.2014.3296